Macro-Prep High S Resin

Macro-Prep High S Resin

Overview

Macro-Prep High S strong cation exchange support contains sulfonate functional groups and is ideal for purifying basic and neutral proteins and peptides.

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Description

Description

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Macro-Prep High S Resin is a strong cation exchange resin containing sulfonate functional groups that is ideal for purifying basic and neutral proteins and peptides. It is an excellent choice for rapid purification that meets the demands of analytical, semipreparative, and process-scale applications.

This methacrylate copolymer bead provides high-resolution separations at very high flow rates. Changes in pH or ionic strength of the buffer do not cause shrinking or swelling of the support. The superior mechanical and chemical stability of Macro-Prep High S Resin make it a preferred choice over other sulfonate cation exchangers on the market. Macro-Prep High S Resin can be sanitized with chaotropic agents and can be autoclaved. It provides consistent and reproducible results.

Features and Benefits

  • High capacity for biomolecules
  • High resolution of complex biological mixtures
  • Rigid polymer matrix that allows high flow rates at moderate pressures
  • Hydrophilic matrix that reduces nonspecific binding
  • Macroporous beads that enhance access to ionic sites
  • High chemical, mechanical, and thermal stability

More Information

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO 9001-registered corporation.

Specifications

Specifications

Properties of Macro-Prep High S Support
 
Type of support
Strong cation exchange
Functional group
–SO3-
Typical dynamic binding capacity*
≥49 lgG/ml
Nominal particle size
50 µm
Nominal pore diameter
1,000 Å
Recommended maximum linear flow rate
3,000 cm/hr
Autoclavability
30 min at 121°C
pH stability
1–10
Regeneration
in 1–2 M NaCl/KCI or 70% ethanol
*Determined with human lgG.
 
Chemical Compatability of Macro-Prep High S Support
 
1% SDS
Yes
8 M guanidine-HCI
Yes
1 N HCI
Yes
100% ethanol
Yes

Ordering

Ordering

items

Use the filters below to refine results!

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1560032 tn
156-0032

5 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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12003671 sku tn
12003671

5 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size, shipped in 2% benzyl alcohol.

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1560033 tn
156-0033

10 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size

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12003672 tn
12003672

10 L, strong cation exchange chromatography media, for analytical, semi-preparative, and process scale applications, 50 μm particle size, shipped in 2% benzyl alcohol.

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EconoFit_Columns-1x1ml-Sku_TN.png
12009276

Pkg of 1, 1 ml, prepacked cation exchange column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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EconoFit_Columns-5x1ml-Sku_TN.png
12009270

Pkg of 5, 1 ml, prepacked cation exchange column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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EconoFit_Columns-1x5ml-Sku_TN.png
12009271

Pkg of 1, 5 ml, prepacked cation exchange column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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EconoFit_Columns-5x5ml-Sku_TN.png
12009272

Pkg of 5, 5 ml, prepacked cation exchange column, 16 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system

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158-0100_tn.jpg
(Discontinued) Media Sampler Pack
1580100
Includes 25 ml each of UNOsphere™ Q and UNOsphere S, Macro-Prep® DEAE, Macro-Prep High Q, and Macro-Prep High S; 5 ml of UNOsphere SUPrA™; 10 g each of CHT™ Ceramic Hydroxyapatite Types I and II, 40 μm; and 10 g of CFT™ Ceramic Fluoroapatite Type II, 40 μm
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125-0562_tn.jpg
1250562
Pkg of 6 vials, lyophilized mixture of equine myoglobin, Ribonuclease A, cytochrome c, for use in ion exchange chromatography

Documents

Documents

Number Description Options
LIT271
Instruction Manual, Macro-Prep Ion Exchange Supports, Rev D
5584
Bio-Scale Mini Cartridges, Product Information Sheet, Rev B
5643
Macro-Prep® High S Support Product Information Sheet, Rev A
6810
Practical Guide: Selecting the Optimal Resins for the Process Purification of Native and Recombinant Proteins, Ver A
6983
Native and Recombinant Protein Purification Resins/Media Selection Card
1985
Clearance of Murine Leukemia Virus From a Chimeric Monoclonal Antibody Using Ion Exchange Chromatography, Rev B.
1913
Developing and Scaling Up a Purification Procedure With Two New Ion Exchange Supports (Poster), Rev B
2026
Evaluation of Different Approaches for the Chromatographic Purification of Monoclonal Antibodies
2079
Non-Protein A Based Purification of MAb 414, Rev B
6868
Macro-Prep® Chromatography Resins Publications List, Ver A
5662
Process Chromatography Applications Laboratory Brochure, Rev B