Macro-Prep 25 S Media

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Overview

Pressure versus Linear Velocity

Pressure vs. linear velocity for 1 M NaCl on Macro-Prep 25 S in a 1.6 cm (ID) x 12.5 cm (L) column.

Macro-Prep 25 S strong cation exchange support offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High S support but in a 25 µm bead that offers higher resolution.

Benefits include:

  • Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
  • Fewer chromatographic steps required
  • Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation
Type of ion exchanger Strong cation
Functional group –SO3-
Total ionic capacity 110±30 µeq/ml
Median particle size 25 µm
Nominal pore size 725 Å
Shipping counterion NA+
Dynamic binding capacity >40 mg/ml lgG
Recommended linear flow rate range 50–300 cm/hr
Maximum linear flow rate 3,000 cm/hr
Chemical stability  
  1.0 M HCI >72 hr
  100% ethanol Excellent
  Chaotropic agents (8 M urea, 6 M guanidine-HCI) Excellent
pH stability 1–12
Volume changes  
  ph 4–10 <1%
  0.1–1.0 M Na Cl <4%
Autoclavability (121°C, 30 min) Yes
Storage solution 20% ethanol
Macro-Prep 25 S Support

153-0031
50 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

List Price:   $769.00
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Macro-Prep 25 S Support

153-0032
200 ml, cation exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

List Price:   $2,702.00
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Macro-Prep 25 S Support

153-0033
1 L, strong cation exchange chromatography media, 25 μm particle size

List Price:   $7,200.00
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Macro-Prep 25 S Support

153-0034
5 L, strong cation exchange chromatography media, 25 μm particle size

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Number Description Options
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
4006129 Instruction Manual, Macro-Prep 25 Ion Exchange Supports, Rev B Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download