Macro-Prep 25 Q Media

Print

Overview

Purification on Macro-Prep 25Q column

Separation of 360 µl of 6xHis-tagged dihydrofolate reductase E. coli lysate on a 2 ml column packed with macro-Prep 25 Q. Buffer A: 59 mM Trist, pH 8.3. Buffer B: 50 mM Trist, pH 8.3, 1 M NaCl; gradient: 0–100% B in 10 column volumes; flow rate: 2 ml/min.

To read more click here

Macro-Prep 25 Q strong anion exchange support offers high-resolution separations at high flow rates with medium pressures. It possesses the same rigid, macroporous, and hydrophilic properties of 50 µm Macro-Prep High Q support but in a 25 µm bead that offers higher resolution.

Benefits include:

  • Benchtop to pilot-scale separations, where maximum sample recovery and purity are important
  • Fewer chromatographic steps required
  • Removal of protein variants and other difficult contaminants that would require additional validation if present in the final formulation
Type of ion exchanger Strong anion
Functional group –N+(CH3)3
Total ionic capacity 220 ± 40 µeq/ml
Median particle size 25 µm
Nominal pore size 725 Å
Shipping counterion Cl-
Dynamic binding capacity >30 mg/ml BSA
Recommended linear flow rate range 50–300 cm/hr
Maximum linear flow rate 3,000 cm.hr 3,000 cm/hr
Chemical stability  
   1.0 M HCI >48 hr
   100% ethanol Excellent
   1.0 M HCI Excellent
pH stability 1–10
Volume changes  
   pH 4–10 <1%
   0.1–1.0 M NaCl <5%
Autoclavability Yes; do not autoclave the OH- form
Storage solution 20% ethanol
Macro-Prep 25 Q Support

153-0021
50 ml, strong anion exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

List Price:   $769.00
Quantity:   Add to cart
Add to Hot List   Add to Quote
Macro-Prep 25 Q Support

153-0022
200 ml, strong anion exchange chromatography media, 25 µm particle size, for high-flow medium-pressure separations

List Price:   $2,768.00
Quantity:   Add to cart
Add to Hot List   Add to Quote
Macro-Prep 25 Q Support

153-0023
1 L, strong anion exchange chromatography media, 25 μm particle size

List Price:   $7,200.00
Quantity:   Add to Quote
Add to Hot List  
Macro-Prep 25 Q Support

153-0024
5 L, strong anion exchange chromatography media, 25 μm particle size

List Price:   Inquire
Quantity:   Add to Quote
Add to Hot List  
Number Description Options
2292 Macro-Prep Ion Exchange Supports Product Information Sheet, Rev A Click to download
4006129 Instruction Manual, Macro-Prep 25 Ion Exchange Supports, Rev B Click to download
5662 Process Chromatography Applications Laboratory Brochure, Rev B Click to download