Immun-Blot® PVDF Membrane

Immun-Blot® PVDF Membrane

Bio-Rad's Immun-Blot PVDF membrane is ideal for chemiluminescent and colorimetric western blots. This PVDF membrane retains target protein very strongly while reducing nonspecific protein binding.

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Overview

Immun-Blot® PVDF Membrane is ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features and Benefits

  • Mechanical strength of Immun-Blot PVDF membrane enables multiple reprobing of blots
  • Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
  • Available in three formats:
    • Convenient precut membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transferring proteins from SDS-PAGE gels to membrane
  • Immunoblotting target proteins
  • Detection by western blotting chemiluminescent or colorimetric methods

Packaging Options

Immun-Blot PVDF Membrane Dimensions Sheets/package Catalog Number
Roll 26 cm x 3.3 m 1620177
Precut for mini gels 7 x 8.4 cm 10 1620174
Precut for midi gels 10 x 15 cm 10 1620175
Precut for PROTEAN II XL Gels 20 x 20 cm 10 1620176
Precut for large-format gels 25 x 28 cm 10 1620255

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1620177
Pkg of 1 roll, 0.2 µm, 26 cm x 3.3 m, bulk membrane for high binding (150–160 µg/cm2) immunoblotting
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1620174
Pkg of 10, 0.2 µm, 7 x 8.4 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
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1620175
Pkg of 10, 0.2 µm, 10 x 15 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
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1620176
Pkg of 10, 0.2 µm, 20 x 20 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting
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1620255

Pkg of 10, 0.2 µm, 25 x 28 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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1620238
Pkg of 20, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels
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1620239
Pkg of 50, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels
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1620218
Pkg of 20, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels
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1620219
Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels

Documents

Number Description Options
2895
Protein Blotting Guide, Ver C
2212
Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C
4006127
Instruction Manual, Immun-Blot PVDF, Rev A