Immun-Blot® PVDF Membrane

Print

Overview

Bio-Rad Protein Transfer Membrane Offerings
Protein Membranes

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

Immun-Blot® PVDF Membrane
  • Ideal for chemiluminescent and colorimetric western blots
  • Retains target protein very strongly during protein transfer but also reduces nonspecific protein binding
Immun-Blot® Low Fluorescence PVDF Membrane
  • Same benefits as standard PVDF membranes plus minimal autofluorescence and high sensitivity
  • Best choice for multiplex fluorescent immunoblotting
Sequi-Blot™ PVDF Membrane
  • Ideal for protein sequencing: retains target protein very strongly during protein transfer
  • Provides the binding capacity to sequence even low-abundance samples
Nitrocellulose Membrane, 0.45 µm
  • Proven medium for western, northern, and Southern blotting
  • 45 µm pore size is recommended for most analytical blotting, including protein, ssDNA, and RNA transfers
  • 0.2 µm pore size is optimized for transferring nucleic acids and low molecular–weight proteins
Nitrocellulose Membrane, 0.2 µm
Supported Nitrocellulose Membrane, 0.45 µm
  • Dense 100% nitrocellulose cast on both sides of an inert polyester support
  • Easy to handle, wets readily, and does not have the brittleness often associated with unsupported nitrocellulose
Supported Nitrocellulose Membrane, 0.2 µm
PVDF Membranes

Ideal for:

  • Protein blotting
    (Immun-Blot® PVDF membrane)
  • Protein sequencing
    (Sequi-Blot™ PVDF membrane)

 

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.



Immun-Blot® PVDF Membrane is ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features and Benefits

  • Mechanical strength of Immun-Blot PVDF membrane enables multiple reprobing of blots
  • Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
  • Available in three formats:
    • Convenient precut membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transferring proteins from SDS-PAGE gels to membrane
  • Immunoblotting target proteins
  • Detection by western blotting chemiluminescent or colorimetric methods

Packaging Options

Immun-Blot PVDF Membrane Dimensions Sheets/package Catalog Number
Roll 26 cm x 3.3 m 1620177
Precut for mini gels 7 x 8.4 cm 10 1620174
Precut for midi gels 10 x 15 cm 10 1620175
Precut for PROTEAN II XL Gels 20 x 20 cm 10 1620176
Precut for large-format gels 25 x 28 cm 10 1620255

Related Products

Related Categories

Online Resources

x

Account price failed to display. Please try reloading the page. Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at   or send an
email to 

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Immun-Blot PVDF Membrane #162-0177
Immun-Blot® PVDF Membrane, Roll, 26 cm x 3.3 m

1620177
Pkg of 1 roll, 0.2 µm, 26 cm x 3.3 m, bulk membrane for high binding (150–160 µg/cm2) immunoblotting

List Price:
 
$396.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF Membrane #162-0174
Immun-Blot® PVDF Membrane, Precut, 7 x 8.4 cm

1620174
Pkg of 10, 0.2 µm, 7 x 8.4 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

List Price:
 
$105.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF Membrane #162-0175
Immun-Blot® PVDF Membrane, Precut, 10 x 15 cm

1620175
Pkg of 10, 0.2 µm, 10 x 15 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

List Price:
 
$152.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF Membrane #162-0176
Immun-Blot® PVDF Membrane, Precut, 20 x 20 cm

1620176
Pkg of 10, 0.2 µm, 20 x 20 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

List Price:
 
$315.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF Membrane #162-0255
Immun-Blot® PVDF Membrane, Precut, 25 x 28 cm

1620255
Pkg of 10, 0.2 µm, 25 x 28 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

List Price:
 
$335.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF/Filter Paper Sandwiches #162-0238
Immun-Blot® PVDF/Filter Paper Sandwiches

1620238
Pkg of 20, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels

List Price:
 
$411.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF/Filter Paper Sandwiches #162-0239
Immun-Blot® PVDF/Filter Paper Sandwiches

1620239
Pkg of 50, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels

List Price:
 
$904.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF/Filter Paper Sandwiches #162-0218
Immun-Blot PVDF/Filter Paper Sandwiches

1620218
Pkg of 20, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:
 
$273.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Immun-Blot PVDF/Filter Paper Sandwiches #162-0219
Immun-Blot PVDF/Filter Paper Sandwiches

1620219
Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels

List Price:
 
$610.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Number Description Options
2895 Protein Blotting Guide, Ver C Click to download
2212 Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C Click to download
1939 Blotting Membrane Brochure, Rev G Click to download
4006127 Instruction Manual, Immun-Blot PVDF, Rev A Click to download