Immun-Blot® PVDF Membrane

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Overview

Available Formats

Bio-Rad's PVDF membranes are available in rolls, precut sheets, or convenient membrane/filter paper sandwiches.

PVDF Membranes

Ideal for:

  • Protein blotting
    (Immun-Blot® PVDF membrane)
  • Protein sequencing
    (Sequi-Blot™ PVDF membrane)

 

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

Immun-Blot® PVDF Membrane is ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features and Benefits

  • Mechanical strength of Immun-Blot PVDF membrane enables multiple reprobing of blots
  • Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
  • Available in three formats:
    • Convenient precut membrane/blot absorbent filter paper sandwiches
    • Packs of precut membrane-only sheets
    • Economical rolls

Applications

  • Transferring proteins from SDS-PAGE gels to membrane
  • Immunoblotting target proteins
  • Detection by western blotting chemiluminescent or colorimetric methods

Packaging Options

Immun-Blot PVDF Membrane Dimensions Sheets/package Catalog Number
Roll 26 cm x 3.3 m 1620177
Precut for mini gels 7 x 8.4 cm 10 1620174
Precut for midi gels 10 x 15 cm 10 1620175
Precut for PROTEAN II XL Gels 20 x 20 cm 10 1620176
Precut for large-format gels 25 x 28 cm 10 1620255

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Immun-Blot PVDF Membrane #162-0177
Immun-Blot® PVDF Membrane, Roll, 26 cm x 3.3 m

1620177
Pkg of 1 roll, 0.2 µm, 26 cm x 3.3 m, bulk membrane for high binding (150–160 µg/cm2) immunoblotting

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$372.00
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Immun-Blot PVDF Membrane #162-0174
Immun-Blot® PVDF Membrane, Precut, 7 x 8.4 cm

1620174
Pkg of 10, 0.2 µm, 7 x 8.4 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$101.00
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Immun-Blot PVDF Membrane #162-0175
Immun-Blot® PVDF Membrane, Precut, 10 x 15 cm

1620175
Pkg of 10, 0.2 µm, 10 x 15 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$140.00
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Immun-Blot PVDF Membrane #162-0176
Immun-Blot® PVDF Membrane, Precut, 20 x 20 cm

1620176
Pkg of 10, 0.2 µm, 20 x 20 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$291.00
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Immun-Blot PVDF Membrane #162-0255
Immun-Blot® PVDF Membrane, Precut, 25 x 28 cm

1620255
Pkg of 10, 0.2 µm, 25 x 28 cm, precut membrane for high binding capacity (150–160 µg/cm2) immunoblotting

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$313.00
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Immun-Blot PVDF/Filter Paper Sandwiches #162-0238
Immun-Blot® PVDF/Filter Paper Sandwiches

1620238
Pkg of 20, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels

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$387.00
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Immun-Blot PVDF/Filter Paper Sandwiches #162-0239
(Discontinued) Immun-Blot® PVDF/Filter Paper Sandwiches

1620239
Pkg of 50, 8.5 x 13.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Criterion™ and Criterion XT precast gels

This product is no longer available. Please contact technical support for more information on replacement product.
Immun-Blot PVDF/Filter Paper Sandwiches #162-0218
Immun-Blot PVDF/Filter Paper Sandwiches

1620218
Pkg of 20, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels

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$249.00
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Immun-Blot PVDF/Filter Paper Sandwiches #162-0219
Immun-Blot PVDF/Filter Paper Sandwiches

1620219
Pkg of 50, 7 x 8.5 cm, precut preassembled blotting membrane/filter paper sandwich for immunoblotting; fits Mini-PROTEAN® and Ready Gel® precast gels

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$574.00
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Number Description Options
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
2212 Application-Specific PVDF Membranes: Immun-Blot PVDF Membrane for Western Blotting and Sequi-Blot PVDF Membrane for Protein Sequencing, Rev C Click to download
1939 Blotting Membrane Brochure, Rev G Click to download [ Add to Cart (Free) ]
4006127 Instruction Manual, Immun-Blot PVDF, Rev A Click to download