Immun-Blot® Opti-4CN™ Colorimetric Kits

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Overview


Specifications
  Amplified Opti-4CN™ Opti-4CN Immun-Blot® HRP (4CN) DAB
Sensitivity 5 pg 100 pg 500 pg 500 pg
Shelf life 1 year 1 year 1 year 1 year
Storage condition 4°C 4°C 4°C 4°C

Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on the blot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over background is observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however, the method's single endpoint result does not allow the multiple exposures of chemiluminescence methods. Colorimetric detection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.

Bio-Rad's HRP-based colorimetric detection systems offer a choice of HRP-conjugated secondary antibodies and HRP-conjugated protein A or protein G using the detection reagent 4-chloro-1-naphthol(4CN). Bio-Rad offers three types of kits based on this chemistry for colorimetric HRP detection; individual reagents are also available, including 3,3'-diaminobenzidine (DAB), an alternative reagent.

Opti-4CN Substrate Kit

170-8235
Colorimetric detection (µg) kit, includes optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)

List Price:   $210.00
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Opti-4CN Goat Anti-Mouse Detection Kit

170-8237
Colorimetric detection (µg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)

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Opti-4CN Goat Anti-Rabbit Detection Kit

170-8236
Colorimetric detection (μg) kit, includes secondary antibody, optimized 4CN substrate; for 2,500 cm2 of membrane (~50 mini blots)

List Price:   $236.00
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Amplified Opti-4CN Substrate Kit

170-8238
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)

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Amplified Opti-4CN Goat Anti-Mouse Detection Kit

170-8240
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, antibody conjugate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)

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Amplified Opti-4CN Goat Anti-Rabbit Detection Kit

170-8239
High sensitivity (5 pg) colorimetric detection kit, includes optimized 4CN substrate, antibody conjugate, amplification reagents; for 2,500 cm2 of membrane (~50 mini blots)

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Immun-Blot Goat Anti-Rabbit IgG (H + L)-HRP Assay Kit

170-6463
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

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Immun-Blot Goat Anti-Mouse IgG (H + L)-HRP Assay Kit

170-6464
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

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Immun-Blot Goat Anti-Human IgG (H + L)-HRP Assay Kit

170-6465
Colorimetric immunodetection kit, includes 4CN, horseradish peroxidase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

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HRP Conjugate Substrate Kit

170-6431
Colorimetric HRP substrate reagent kit, includes premixed 4CN, hydrogen peroxide solutions, color development buffer; makes 1 L color development solution

List Price:   $128.00
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HRP Color Development Reagent

170-6534
5 g, 4-chloro-1-naphthol (4CN), colorimetric blotting substrate; used with peroxidase antibody conjugate

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HRP Color Development Reagent

170-6535
5 g, 3,3'-diaminobenzidine (DAB), colorimetric blotting substrate; used with peroxidase antibody conjugate

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Number Description Options
9178 Instruction Manual, Preparation of DAB Color Development Solution for Use With the Immun-Blot Horseradish Peroxidase Assay, Rev B Click to download
LIT178 Instruction Manual, HRP Conjugate Substrate Kit, Rev C Click to download
LIT387 Instruction Manual, HRP Color Development Solution, DAB (diaminobenzidine) Click to download
LIT320 Instruction Manual, HRP Color Development Solution 4CN (4-chloro-1-naphthol), Rev B Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
2260 Opti-4CN and Amplified Opti-4CN Protein Blotting Kits Flier, Rev A Click to download
4100130 Instruction Manual, Opti-4CN and Amplified Opti-4CN Kits, Rev C Click to download