Immun-Blot® AP Colorimetric Kits

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Overview



Specifications
Substrate Sensitivity Color Product Options
BCIP/NBT 100 pg Purple Dry powder, liquid substrate, Immun-Blot kits
Colorimetric Chemistry

Colorimetric detection. The secondary antibody, which binds the antibody specific for the protein of interest, is conjugated to an enzyme; its substrate is converted to a colored precipitate.

Enzymes such as HRP or AP convert several substrates to a colored precipitate. As the precipitate accumulates on the blot, a colored signal develops. The reaction can be monitored and stopped when the desired signal over background is observed. Colorimetric detection is easier to perform than film-based chemiluminescence detection; however, the method's single endpoint result does not allow the multiple exposures of chemiluminescence methods. Colorimetric detection is typically considered a medium-sensitivity method compared to radioactive or chemiluminescence detection.

Bio-Rad's AP-based colorimetric kits utilize 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue Tetrazolium (BCIP/NBT), which reacts with the alkaline phosphatase to form a color precipitate for detection. The Immun-Blot AP assay kit includes all necessary reagents for colorimetric detection and can detect up to 100 pg of protein.

Immun-Blot Goat Anti-Rabbit IgG (H + L)-AP Assay Kit

170-6460
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

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Immun-Blot Goat Anti-Mouse IgG (H + L)-AP Assay Kit

170-6461
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

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Immun-Blot Goat Anti-Human IgG (H + L)-AP Assay Kit

170-6462
Colorimetric immunodetection kit, includes BCIP/NBT, alkaline phosphatase secondary antibody conjugate, 10x TBS, Tween 20, gelatin; for 200 assays

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Blotting-Grade Biotinylated AP Conjugate

170-6403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate

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AP Conjugate Substrate Kit

170-6432
Colorimetric AP substrate reagent kit, includes premixed BCIP and NBT solutions, color development buffer; makes 1 L color development solution

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Hazardous  
AP Color Development Reagent

170-6539
300 mg, 5-bromo-4-chloro-3-indolyl phosphate (BCIP), colorimetric substrate, for purple color development; requires AP color development reagent (#170-6532)

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AP Color Development Reagent

170-6532
600 mg, nitroblue tetrazolium (NBT), colorimetric substrate, for purple color development; requires AP color development reagent (#170-6539)

List Price:   $115.00
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Number Description Options
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
LIT169 Instruction Manual, Alkaline Phosphatase Conjugate Substrate Kit, Rev E Click to download
LIT171 Instruction Manual, Immun-Blot Kit Click to download