IDEA Kit — Inquiry Dye Electrophoresis Activity

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IDEA Kit — Inquiry Dye Electrophoresis Activity - DNA Analysis Kits and Agarose Gel Electrophoresis Kits

Overview

IDEA Kit — Inquiry Dye Electrophoresis Activity Quick Guide

Download the IDEA Kit — Inquiry Dye Electrophoresis Activity Quick Guide for the color version of the student protocol.

 

IDEA Kit — Inquiry Dye Electrophoresis Activity Flowchart

Lab Preparation Checklist
Kit Contents (sufficient materials for 8 student workstations with 2–4 students per workstation)
Store at room temperature
Dye extraction solution, 25 ml 1
Blue 1 reference dye, 150 µl 1
Yellow 5 reference dye, 150 µl 1
Yellow 6 reference dye, 150 µl 1
Red 40 reference dye, 150 µl 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Molecular biology grade agarose, 5 g 1
Microcentrifuge tubes, 2 ml 72
Curriculum (includes teacher guide, student manual, and graphic quick guide) 1
Required Accessories Not Included in Kit
2–20 µl adjustable-volume micropipets or 10 µl fixed-volume micropipets 8
2–200 µl pipet tips 1 bag
Horizontal gel electrophoresis chambers 8
Eyedroppers 8
Power supplies 2–4
Permanent markers 8
Plastic cups or small beakers 32
Microwave oven or hot plate 1
Distilled water 3 liters
500 ml Erlenmeyer flask for microwaving agarose 1
Laboratory tape 1
Candies with a variety of color coatings Variable
Balance 1
Recommended (Optional) Accessories
Microcentrifuge tube racks 8
Microcentrifuge or mini centrifuge 1
Precast agarose gels 8
100–1,000 µl adjustable-volume micropipets 8
Disposable plastic transfer pipets 40
100–1,000 µl pipet tips 1 bag
Digital camera for imaging gels 1
Key Kit Features
  • Inquiry-based hands-on laboratory
  • Kit can be completed in two 45-min laboratory sessions
  • Visible results
  • Real-world connection
Curriculum Fit

Create context. Reinforce learning. Stay current. New scientific discoveries and technologies create more content for you to teach, but not more time. Biotechnology Explorer™ kits help you teach more effectively by integrating multiple core content subjects into a single lab. Connect concepts with techniques and put them into context with real-world scenarios.

Agarose gel electrophoresis is a fundamental technique used daily in the molecular biology lab to assess DNA. Teach this skill in a fun way with inquiry!

Food for Thought
The IDEA kit — inquiry dye electrophoresis activity is the first dye electrophoresis laboratory activity that encourages inquiry by allowing students to explore and provide samples from everyday foods — candy! The kit covers the basic skills necessary to perform electrophoresis, including pipetting, making solutions, and casting and running agarose gels, and comparing unknown samples to the provided known samples to determine which dyes are in their candies. Students will discover how molecules move through the gel matrix, and how physical and/or chemical properties are linked to the distance molecules migrate. This kit will also open up the conversation about the use of food dyes, the different types of food dyes, and what is in the foods your students consume.

Features and Benefits

  • Inquiry-based hands-on laboratory
  • Kit can be completed in two 45-min laboratory sessions
  • Visible results
  • Real-world connection

Applications and Uses

This kit offers learning opportunities for all levels of instruction:

  • For secondary and college level instruction, lessons about agarose gel electrophoresis and its accompanying skills can be introduced or reinforced with this laboratory
  • For middle school instruction, the IDEA kit is a gateway to inquiry-based hands-on science experiences

More Information

The IDEA kit provides sufficient materials for eight student workstations with up to four students per workstation.

Blue 1 reference dye, µl 150
Yellow 5 reference dye, µl 150
Yellow 6 reference dye, µl 150
Red 40 reference dye, µl 150
Dye extraction solution, ml 25
Microcentrifuge tubes, 2 ml 72
Curriculum 1 (includes teacher guide, student manual, and graphic quick guide)
IDEA Kit — Inquiry Dye Electrophoresis Activity

166-5075EDU
Introductory agarose gel electrophoresis kit, includes reference dyes, extraction buffer, microcentrifuge tubes, agarose, TAE, curriculum; education use only

List Price:   $85.00
EDU Price:   $68.00   What is this?
Quantity:   Add to cart
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IDEA Kit Starter Pack

166-5077EDU
Introductory agarose gel electrophoresis kit, includes reference dyes, extraction buffer, microcentrifuge tubes, agarose, TAE, fixed-volume pipets, tips, curriculum; education use only

List Price:   $282.00
EDU Price:   $225.60   What is this?
Quantity:   Add to cart
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Number Description Options
6287 Instruction Manual, IDEA Kit — Inquiry Dye Electrophoresis Activity Quick Guide, Biotechnology Explorer, Rev A Click to download
1665078EDU Instruction Manual, IDEA Kit — Inquiry Dye Electrophoresis Activity, Biotechnology Explorer, Rev A Please login to download this document