Hydroxyproline by HPLC (Reversed Phase)

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Hydroxyproline by HPLC (Reversed Phase)

Bio-Rad's Hydroxyproline by HPLC assay is intended for the quantitative determination of hydroxyproline in urine. It requires only an 18-hour collagen-free diet, and a 2-hour urine collection.

Hydroxyproline is a frequently used marker for bone density. A high secretion of hydroxyproline is of clinical importance and is a signal of destructive processes in the skeletal system. Measurement of hydroxyproline allows early diagnosis and control of progression of bone metastases of malignant diseases, as well as control of progression or therapy of osteoporosis, acromegaly, rheumatoid arthritis, or Morbus Paget, and diagnosis of hyperparathyroidism.

Test Characteristics:

  • Principle: Isocratic HPLC method (Reversed Phase)
  • Duration of chromatography: 6 minutes
  • Sample volume: 1 mL urine
  • Flow rate: 1.5 mL/min
  • UV Detection: 471 nm
Hydroxyproline by HPLC Reagent Kit
Hydroxyproline by HPLC Reagent Kit

195-9501
Assay for the quantitative determination of hydroxyproline in urine by HPLC (Reversed Phase), (100 tests)

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Reagent Set
Reagent Set

195-9501R
Reagent set for hydroxyproline by HPLC assay (1 unit)

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Mobile Phase for Hydroxyproline by HPLC Reagent Kit
Mobile Phase for Hydroxyproline by HPLC Reagent Kit

195-9515
Mobile phase for hydroxyproline by HPLC assay (2 x 2000 mL)

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Hydrolysis glass tubes
Hydrolysis glass tubes

195-9509
Glass tube set for precolumn sample derivatization prior to assay of hydroxyproline by HPLC (100 tubes)

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Analytical Column for Hydroxyproline by HPLC Reagent Kit
Analytical Column for Hydroxyproline by HPLC Reagent Kit

195-9520
One analytical column with 2 microguard cartridges for hydroxyproline by HPLC assay (1 package; 500 injections)

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Micro-Guard Cartridge Holder

195-5030
1 holder for high performance liquid chromatography guard cartridge

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Capillary Connection Set
Capillary Connection Set

195-4088
Set of hardware to connect 2 HPLC cartridges (1 unit)

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Test Mix for Hydroxyproline by HPLC Reagent Kit

195-9502
Test mixture for hydroxyproline by HPLC assay (1 x 5 mL)

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Urine calibrator for Hydroxyproline by HPLC Reagent Kit
Urine calibrator for Hydroxyproline by HPLC Reagent Kit

195-9504
Urine calibrator for hydroxyproline by HPLC assay (1 x 15 mL)

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Micro-Guard Refill Cartridges

195-6003
Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections

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Lyphochek Quantitative Urine Control, Level 1, Normal

376
Assayed, lyophilized, human urine based control for urine chemistry analytes, amines, and hormones; normal level (12 x 10 mL)

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Lyphochek™ Quantitative Urine Control, Level 2, Abnormal

377
Assayed, lyophilized, human urine based control for urine chemistry analytes, amines, and hormones; abnormal level (12 x 10 mL)

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