HRP and AP Conjugates

Print
HRP and AP conjugates for western blotting

Overview

Conjugate Specifications
Products Volume Storage Condition Shelf Life Recommended Dilution
Avidin-HRP 2 ml 4°C 1 year 1:1000–1:3000
Goat Anti-Rabbit (H+L) 2 ml 4°C 1 year 1:3000
Goat Anti-Mouse (H+L) 2 ml 4°C 1 year 1:3000
Goat Anti-Human (H+L) 2 ml 4°C 1 year 1:3000
Protein A-HRP 1 ml 4°C 1 year 1:3000
Protein G-HRP 1 ml 4°C 1 year 1:3000
         
Goat Anti-Rabbit IgG-AP 1 ml 4°C 1 year 1:3000
Goat Anti-Mouse IgG-AP 1 ml 4°C 1 year 1:3000
Goat Anti-Human IgG-AP 1 ml 4°C 1 year 1:3000
Avidin-AP 1 ml 4°C 1 year 1:1000–1:3000
         
Biotinylated AP 1 ml 4°C 1 year 1:3000
Biotinylated GAR (H+L), human IgG adsorbed 1 ml 4°C 1 year 1:3000
Antibody Binding Affinities of Protein A and Protein G
Monoclonal Protein A Protein G
Human IgG1 +++ +++
Human IgG2 +++ +++
Human IgG3 +++
Human IgG4 +++ +++
Mouse IgG1 + +++
Mouse IgG2a +++ +++
Mouse IgG2b ++ ++
Mouse IgG3 ++ ++
Rat IgG1 +
Rat IgG2aa +++
Rat IgG2b ++
Rat IgG2c + ++
Polyclonal Protein A Protein G
Rabbit +++ ++
Cow ++ +++
Horse ++ +++
Goat ++
Guinea pig +++ ++
Sheep +/- ++
Pig ++ ++
Rat +/- ++
Mouse ++ ++
Chicken +
Human IgG +++ +++
Human IgM +
Human IgD +
Human IgA +

– No binding
+ Weak binding
++ Moderate binding
+++ Strong binding

Binding affinities of protein A and G to various species and subclasses of polyclonal and monoclonal antibodies.

Blotting-grade HRP and AP conjugates produce specific results, eliminating false positives in western blotting immunoassays

  • Double affinity-purified blotting-grade antibodies are isolated by affinity chromatography and further purified by cross-adsorption against an unrelated species to eliminate nonspecific immunoglobulins
  • The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3000) decreases background and increase the signal-to-noise ratio of the assay
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate

170-6515
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate

List Price:   $209.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Goat Anti-Mouse IgG (H + L)-HRP Conjugate

170-6516
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate

List Price:   $209.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Goat Anti-Human (H+L) HRP conjugate

172-1050
2 ml, EIA-grade, enzyme-antibody conjugate

List Price:   $178.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Protein A-HRP Conjugate

170-6522
1 ml, blotting-grade protein A horseradish peroxidase antibody conjugate

List Price:   $190.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Protein G-HRP Conjugate

170-6425
1 ml, blotting-grade protein G horseradish peroxidase antibody conjugate

List Price:   Log in
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Avidin-HRP

170-6528
2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards

List Price:   $128.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate

170-5047
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

List Price:   $233.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate

170-5046
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

List Price:   $233.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Goat Anti-Rabbit IgG-AP Conjugate

170-6518
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

List Price:   $215.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Goat Anti-Mouse IgG-AP Conjugate

170-6520
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

List Price:   $215.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Goat Anti-Human IgG-AP Conjugate

170-6521
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

List Price:   Log in
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Avidin-AP

170-6533
1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate

List Price:   Log in
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Blotting-Grade Biotinylated AP Conjugate

170-6403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate

List Price:   Log in
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
Lit423 Intruction Manual, Blotting-Grade Avidin-Horseradish Peroxidase Conjugate, Rev E Click to download
5576 Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C Click to download [ Add to Cart (Free) ]
Lit417 Instruction Manual, Blotting Grade Protein G - Horseradish Peroxidate Conjugate, Rev C Click to download
LIT422 Instruction Manual, Blotting Grade Protein A- Horseradish Peroxidase Conjugate Click to download
LIT418 Instruction Manual, Blotting Grade Goat Anti-Rabbit IgG (H + L) (Human IgG Adsorbed) Horseradish Peroxidase Conjugate Click to download [ Add to Cart (Free) ]
LIT419 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Rabbit IgG (H + L) Alkaline Phosphatase Conjugate Click to download
LIT372 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Horseradish Peroxidase Conjugate, Rev D Click to download
LIT420 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Alkaline Phosphatase Conjugate, Rev D Click to download
LIT421 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG (H + L) Alkaline Phosphatase Conjugate Click to download
LIT440 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG ((H + L) Horseradish Peroxidase Conjugate Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
9137 Instruction Manual, Avidin-Alkaline Phosphatase (AP) Conjugate Click to download