HRP and AP Conjugates

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Overview

Conjugate Specifications
Products Volume Storage Condition Shelf Life Recommended Dilution
Avidin-HRP 2 ml 4°C 1 year 1:1000–1:3000
Goat Anti-Rabbit (H+L) 2 ml 4°C 1 year 1:3000
Goat Anti-Mouse (H+L) 2 ml 4°C 1 year 1:3000
Goat Anti-Human (H+L) 2 ml 4°C 1 year 1:3000
Protein A-HRP 1 ml 4°C 1 year 1:3000
Protein G-HRP 1 ml 4°C 1 year 1:3000
         
Goat Anti-Rabbit IgG-AP 1 ml 4°C 1 year 1:3000
Goat Anti-Mouse IgG-AP 1 ml 4°C 1 year 1:3000
Goat Anti-Human IgG-AP 1 ml 4°C 1 year 1:3000
Avidin-AP 1 ml 4°C 1 year 1:1000–1:3000
         
Biotinylated AP 1 ml 4°C 1 year 1:3000
Biotinylated GAR (H+L), human IgG adsorbed 1 ml 4°C 1 year 1:3000
Binding Specificities
Immunoglobulin Protein A Protein G
Human IgG1
Human IgG2
Human IgG3
Human IgG4
Mouse IgG1 o / — o
Mouse IgG2a
Mouse IgG2b
Mouse IgG3
Rat IgG1 o o
Rat IgG2a
Rat IgG2b o
Rat IgG2o

• Strong binding
o Weak binding
— No binding

Blotting-grade conjugates produce specific results, eliminating false positives in western blotting immunoassays

  • Double affinity-purified blotting-grade antibodies are isolated by affinity chromatography and further purified by cross-adsorption against an unrelated species to eliminate nonspecific immunoglobulins
  • The high titer of the blotting-grade antibody conjugates increase assay sensitivity. Greater working dilutions (1:3000) decreases background and increase the signal-to-noise ratio of the assay
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate

170-6515
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate

List Price:   $209.00
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Goat Anti-Mouse IgG (H + L)-HRP Conjugate

170-6516
2 ml, blotting-grade horseradish peroxidase secondary antibody conjugate

List Price:   $209.00
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Goat Anti-Human (H+L) HRP conjugate

172-1050
2 ml, EIA-grade, enzyme-antibody conjugate

List Price:   $178.00
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Protein A-HRP Conjugate

170-6522
1 ml, blotting-grade protein A horseradish peroxidase antibody conjugate

List Price:   $190.00
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Protein G-HRP Conjugate

170-6425
1 ml, blotting-grade protein G horseradish peroxidase antibody conjugate

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Avidin-HRP

170-6528
2 ml, HRP-Avidin conjugate for detection of biotinylated SDS-PAGE standards

List Price:   $128.00
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Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate

170-5047
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

List Price:   $233.00
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Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate

170-5046
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

List Price:   $233.00
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Goat Anti-Rabbit IgG-AP Conjugate

170-6518
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

List Price:   $215.00
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Goat Anti-Mouse IgG-AP Conjugate

170-6520
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

List Price:   $215.00
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Goat Anti-Human IgG-AP Conjugate

170-6521
1 ml, blotting-grade alkaline phosphatase secondary antibody conjugate

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Avidin-AP

170-6533
1 ml, blotting-grade alkaline phosphatase (AP) avidin conjugate

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Blotting-Grade Biotinylated AP Conjugate

170-6403
1 ml, alkaline phosphatase (AP) secondary antibody conjugate

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Number Description Options
Lit423 Intruction Manual, Blotting-Grade Avidin-Horseradish Peroxidase Conjugate, Rev E Click to download [ Add to Cart (Free) ]
5576 Molecular Weight Estimation and Quantitation of Protein Samples Using Precision Plus Protein WesternC Standards, the Immun-Star WesternC Chemiluminescent Detection Kit, and the Molecular Imager ChemiDoc XRS Imaging System, Rev C Click to download [ Add to Cart (Free) ]
Lit417 Instruction Manual, Blotting Grade Protein G - Horseradish Peroxidate Conjugate, Rev C Click to download [ Add to Cart (Free) ]
LIT422 Instruction Manual, Blotting Grade Protein A- Horseradish Peroxidase Conjugate Click to download [ Add to Cart (Free) ]
LIT418 Instruction Manual, Blotting Grade Goat Anti-Rabbit IgG (H + L) (Human IgG Adsorbed) Horseradish Peroxidase Conjugate Click to download [ Add to Cart (Free) ]
LIT419 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Rabbit IgG (H + L) Alkaline Phosphatase Conjugate Click to download [ Add to Cart (Free) ]
LIT372 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Horseradish Peroxidase Conjugate, Rev D Click to download
LIT420 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Mouse IgG (H + L) Alkaline Phosphatase Conjugate, Rev D Click to download [ Add to Cart (Free) ]
LIT421 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG (H + L) Alkaline Phosphatase Conjugate Click to download [ Add to Cart (Free) ]
LIT440 Instruction Manual, Blotting Grade Affinity Purified Goat Anti-Human IgG ((H + L) Horseradish Peroxidase Conjugate Click to download [ Add to Cart (Free) ]
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
9137 Instruction Manual, Avidin-Alkaline Phosphatase (AP) Conjugate Click to download