Homocysteine by HPLC (Reversed Phase)

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Homocysteine by HPLC (Reversed Phase)

Bio-Rad’s Homocysteine by HPLC application provides a rapid, cost-effective method for quantitative homocysteine analysis of serum or plasma. The presence of elevated total homocysteine levels in plasma or serum has been connected to the increased risk of thrombosis, neural tube defects and atherosclerotic vascular disease. This assay features one-step derivatization and reduction, 5-minute incubation, unique internal standard, and fluorescence labeling performed with thiol-specific dye.

Test characteristics:

  • Principle: Isocratic HPLC method (Reversed Phase)
  • Duration of chromatography: 5 minutes
  • Sample volume: 50 µL plasma or serum
  • Flow rate: 0.7 mL/min.
  • Fluorescence Detection: exitation: 385 nm, emission: 515 nm

 

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Analytical Cartridge Holder

195-5023
Holder for separation cartridge used for determination of homocysteine by high performance liquid chromatography

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Micro-Guard Cartridge Holder

195-5030
1 holder for high performance liquid chromatography guard cartridge

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Capillary Connection Set
Capillary Connection Set

195-4088
Set of hardware to connect 2 HPLC cartridges (1 unit)

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Micro-Guard Refill Cartridges

195-6003
Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections

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