Bio-Rad’s Homocysteine by HPLC application provides a rapid, cost-effective method for quantitative homocysteine analysis of serum or plasma. The presence of elevated total homocysteine levels in plasma or serum has been connected to the increased risk of thrombosis, neural tube defects and atherosclerotic vascular disease. This assay features one-step derivatization and reduction, 5-minute incubation, unique internal standard, and fluorescence labeling performed with thiol-specific dye.
- Principle: Isocratic HPLC method (Reversed Phase)
- Duration of chromatography: 5 minutes
- Sample volume: 50 µL plasma or serum
- Flow rate: 0.7 mL/min.
- Fluorescence Detection: exitation: 385 nm, emission: 515 nm