Homocysteine by HPLC (Reversed Phase)

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Homocysteine by HPLC (Reversed Phase)

Bio-Rad’s Homocysteine by HPLC application provides a rapid, cost-effective method for quantitative homocysteine analysis of serum or plasma. The presence of elevated total homocysteine levels in plasma or serum has been connected to the increased risk of thrombosis, neural tube defects and atherosclerotic vascular disease. This assay features one-step derivatization and reduction, 5-minute incubation, unique internal standard, and fluorescence labeling performed with thiol-specific dye.

Test characteristics:

  • Principle: Isocratic HPLC method (Reversed Phase)
  • Duration of chromatography: 5 minutes
  • Sample volume: 50 µL plasma or serum
  • Flow rate: 0.7 mL/min.
  • Fluorescence Detection: exitation: 385 nm, emission: 515 nm

 

Homocysteine by HPLC Reagent Kit

195-4075
Reagents and mobile phase for determination of homocysteine by high performance liquid chromatography (100 tests)

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Analytical Cartridge

195-4076
Package of 1 separation cartridge, 2 guard cartridges for high performance liquid chromatography of homocysteine

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Analytical Cartridge Holder

195-5023
Holder for separation cartridge used for determination of homocysteine by high performance liquid chromatography

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Micro-Guard Cartridge Holder

195-5030
1 holder for high performance liquid chromatography guard cartridge

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Capillary Connection Set
Capillary Connection Set

195-4088
Set of hardware to connect 2 HPLC cartridges (1 unit)

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Test Mix for Homocysteine by HPLC Kit
Test Mix for Homocysteine by HPLC Kit

195-4089
Test mixture for Homocysteine by HPLC (1 x 2 mL)

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Serum calibrator set for Homocysteine by HPLC Kit
Serum calibrator set for Homocysteine by HPLC Kit

195-4082
Serum calibrator set for Homocysteine by HPLC (2 x 1 mL)

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Mobile Phase

195-4077
Mobile phase for determination of homocysteine by high performance liquid chromatography (700 mL)

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Micro-Guard Refill Cartridges

195-6003
Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections

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Homocysteine Control Set, Bilevel

195-4085
2 vials each of 1.0 mL of control for 2 levels of homocysteine

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