High-Fidelity PCR Kits

High-Fidelity PCR Kits

High-fidelity PCR reagents featuring the thermostable iProof Polymerase. This enzyme utilizes Sso7d fusion polymerase technology to provide highly accurate amplification of targets up to 37 kb.

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Overview

iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.

Features and Benefits of iProof High-Fidelity DNA Polymerase:

  • High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
  • Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
  • Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)

Choose the best DNA polymerase for your application.

Key Features
iProof DNA Polymerase
iTaq DNA Polymerase
Speed 3 of 3 star 2 of 3 star
Processivity 3 of 3 star 2 of 3 star
Long fragment amplification 3 of 3 star 2 of 3 star
Proofreading Yes No
Hot-Start No Yes

 

Related Categories

Supporting Documentation

Specifications

Storage at –20°C
iProof™ Enzyme, Kit, and Buffers: guaranteed for 6 months at –20°C in a constant-temperature freezer
iProof Master Mixes: guaranteed for 3 months at –20°C in a constant-temperature freezer (multiple freeze/thaws not recommended)
Storage at +4°C
Guaranteed for 3 months at 4°C

Ordering

items

Use the filters below to refine results!

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(Discontinued) iProof HF Master Mix, 100 x 50 µl rxns, 2.5 ml
1725310
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates
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(Discontinued) iProof GC Master Mix, 100 x 50 µl rxns, 2.5 ml
1725320
100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates
172-5300_tn.jpg
(Discontinued) iProof™ High-Fidelity DNA Polymerase, 20 U (2 U/µl), 10 µl
1725300
20 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
The following replacement is recommended:
  • iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl ( 1725301 )
172-5301_tn.jpg
(Discontinued) iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl
1725301
100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
172-5302_tn.jpg
(Discontinued) iProof™ High-Fidelity DNA Polymerase, 500 U (2 U/µl), 250 µl
1725302
500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
172-5330_tn.jpg
(Discontinued) iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl
1725330
50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard
172-5331_tn.jpg
(Discontinued) iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl
1725331
200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, λ DNA, 1.3 and 10 kb primers, DNA standard

Documents

Number Description Options
10002300
Instruction Manual, iProof High-Fidelity PCR Kit, Rev B
10002298
Instruction Manual, iProof High-Fidelity DNA Polymerase, Rev B
5211
iProof High-Fidelity DNA Polymerase Flier, Rev C
6252
Reagent Comparison Guide for Real-Time PCR Brochure, Rev C
[ Add to Cart (Free) ]
6090
Amplification Reagents and Plastics Brochure
5337
Long Inverse PCR Using iProof Polymerase, Rev A
10000083948
Instruction Manual, iProof™ High-Fidelity PCR Master Mix