Hard-Shell® 96-Well Skirted PCR Plates, Low-Profile

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Overview

Plate Dimensions
Specifications
Microplate Dimensions Hard-Shell® Low-Profile 96-Well Skirted PCR Plates (HSP-9xxx)
Length at base plane 127.76 mm
Width at base plane 85.48 mm
Height overall 16.06 mm
Well depth 14.81 mm
Well diameter at opening 5.46 mm
Well diameter at bottom of conical section 2.64 mm
Well volume 200 μl
Well spacing 9.00 mm
Well angle 17.5°
Well offset
  Left edge to well A1 14.38 mm
  Top edge to well A1 11.24 mm
  Left edge to H12 113.38 mm
  Top edge to H12 74.24 mm
High-Profile vs. Low-Profile

 

There are two common well heights for PCR tubes and plates:

  1. High-profile (full-height; around 20.7 mm) wells are “standard” and fit in most classical thermal cyclers and real-time PCR detection systems, such as Bio-Rad's iCycler®, iQ™ series real-time PCR detection systems, and Applied Biosystems' regular PCR systems and DNA sequencers.
  2. Low-profile (around 15.5 mm) wells are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR. They have been recommended for newer instruments such as Bio-Rad's C1000 Touch™ and S1000™ thermal cyclers, CFX series real-time PCR detection systems, and Applied Biosystems' fast PCR systems.

Instruments with adjustable lids can usually use both high-profile and low-profile wells. These instruments include Bio-Rad's 1000-series and DNA Engine® series thermal cyclers.

Hard-Shell 96-well skirted PCR plates are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation. The low-profile (16.05 mm) wells are optimized for low-volume reactions and fast PCR.

Key Features and Benefits

  • Reaction volumes of 5–125 µl (200 µl maximum)
  • Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
  • Full skirt for robotic handling and labeling surface
  • Footprint and well spacing match ANSI/SBS standard dimensions
  • Black lettering for easy well identification
  • Process sampling and testing to ensure plates are free of DNase, RNase, and human DNA

Options

  • White-well option for increased fluorescent signal strength
  • Color-coded skirt options with clear and white wells
  • Low-cost, user-readable bar code option for convenient database tracking

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

Well/tube format 96-well
Well/tube profile Low
Skirt format Full-skirted
Compatible instruments Bio-Rad CFX96™
Bio-Rad 1000 series
Bio-Rad Opticon™ series
Bio-Rad Chromo4™
Bio-Rad PTC-100®
Bio-Rad DNA Engine® series
Eppendorf Mastercycler series
Idaho Technology LightScanner
Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design

List Price:   $284.00
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

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Pkg of 50, white shell/clear well PCR plate, bar-coded, 7-digit sequential, Code 128, rigid 2-component design

List Price:   $300.00
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Hard-Shell® Thin-Wall 96-Well Skirted PCR Plates for Abbott PLEX-ID

HSA-9601
Pkg of 50, white shell/clear well PCR plate, rigid 2-component design, designed for use with Abbott's PLEX-ID bioidentification system

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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9611
Pkg of 50, red shell/clear well PCR plate, rigid 2-component design

List Price:   $284.00
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9621
Pkg of 50, yellow shell/clear well PCR plate, rigid 2-component design

List Price:   $284.00
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9631
Pkg of 50, blue shell/clear well PCR plate, rigid 2-component design

List Price:   $284.00
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9641
Pkg of 50, green shell/clear well PCR plate, rigid 2-component design

List Price:   $284.00
Quantity:   Add to cart
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9661
Pkg of 50, black shell/clear well PCR plate for higher fluorescent signals, rigid 2-component design

List Price:   $284.00
Quantity:   Add to cart
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9655
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

List Price:   $313.00
Quantity:   Add to cart
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9955
Pkg of 50, white shell/white well PCR plate for higher fluorescent signals, bar-coded, 7-digit sequential, Code 128, rigid 2-component design

List Price:   $320.00
Quantity:   Add to cart
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9665
Pkg of 50, black shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

List Price:   $313.00
Quantity:   Add to cart
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9635
Pkg of 50, blue shell/white well PCR plate, rigid 2-component design

List Price:   $313.00
Quantity:   Add to cart
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9645
Pkg of 50, green shell/white well PCR plate, rigid 2-component design

List Price:   $313.00
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Hard-Shell® Low-Profile Thin-Wall 96-Well Skirted PCR Plates

HSP-9666
Pkg of 50, black shell/black well PCR plate, rigid 2-component design

List Price:   $313.00
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Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
03526 Instruction Manual, Frame-Seal Incubation Chambers Click to download
03521 Multiplate 96- and 48-Well Unskirted PCR Plates Product Insert, Rev E Click to download
06366 Instruction Manual, Hard-Shell 96-Well Thin-Wall PCR Plates, Rev C Click to download
5496 Hard-Shell PCR Plates Brochure, Rev D Click to download
10012853 Hard-Shell 96-Well PCR Plates Product Insert, Rev D Click to download