Hard-Shell® 96-Well Semi-Skirted PCR Plates, High-Profile

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Overview

Plate Dimensions
Specifications
Microplate Dimensions Hard-Shell® High-Profile 96-Well Semi-Skirted PCR Plates (HSS-9xxx)
Length at top plane 127.76 mm
Width at top plane 85.47 mm
Height overall 20.75 mm
Well depth 19.85 mm
Well diameter at opening 5.50 mm
Well diameter at bottom of conical section 2.69 mm
Well volume 350 μl
Well spacing 9.00 mm
Well angle 17.5°
Well offset
  Left edge to well A1 14.37 mm
  Top edge to well A1 11.23 mm
  Left edge to H12 113.37 mm
  Top edge to H12 74.23 mm
High-Profile vs. Low-Profile

 

There are two common well heights for PCR tubes and plates:

  1. High-profile (full-height; around 20.7 mm) wells are “standard” and fit in most classical thermal cyclers and real-time PCR detection systems, such as Bio-Rad's iCycler®, iQ™ series real-time PCR detection systems, and Applied Biosystems' regular PCR systems and DNA sequencers.
  2. Low-profile (around 15.5 mm) wells are the newer design that reduces the potential for condensate formation and offers advantages for light capture in fluorescence assays, low-volume reactions, and fast PCR. They have been recommended for newer instruments such as Bio-Rad's C1000 Touch™ and S1000™ thermal cyclers, CFX series real-time PCR detection systems, and Applied Biosystems' fast PCR systems.

Instruments with adjustable lids can usually use both high-profile and low-profile wells. These instruments include Bio-Rad's 1000-series and DNA Engine® series thermal cyclers.

Hard-Shell 96-well semi-skirted PCR plates are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation. The full-height (20.75 mm) wells fit most thermal cyclers, real-time PCR detection systems, and DNA sequencers.

Key Features and Benefits

  • Reaction volumes of 5–125 µl (350 µl maximum)
  • Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
  • Warp-free half-height skirt for improved robotic handling and labeling surface
  • Black lettering for easy well identification
  • Process sampling and testing to ensure plates are free of DNase, RNase, and human DNA

Options

  • White-well option for increased fluorescent signal strength
  • Color-coded skirt option with clear wells
  • Low-cost, user-readable bar code option for convenient database tracking

* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.

Well/tube format 96-well
Well/tube profile High
Skirt format Semi-skirted
Compatible instruments Bio-Rad 1000 series
Bio-Rad DNA Engine® series
Bio-Rad iQ™ series
Bio-Rad Chromo4™
Bio-Rad PTC-100®
Applied Biosystems standard systems (7300, 7500, 9700, 2070, Veriti, etc.)
Applied Biosystems Sanger sequencers (3700, 3730, etc.)
Eppendorf Mastercyclers
Hard-Shell® High-Profile 96-Well Semi-Skirted PCR Plates

HSS-9601
Pkg of 25, clear shell/clear well PCR plate to fit most instruments, rigid 2-component design

List Price:   $122.00
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Hard-Shell® Full-Height 96-Well Semi-Skirted PCR Plates

HSS-9641
Pkg of 25, green shell/clear well PCR plate to fit most instruments, rigid 2-component design

List Price:   $123.00
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Hard-Shell Full-Height 96-Well Semi-Skirted PCR Plates

HSS-9665
Pkg of 25, black shell/white well PCR plate for higher fluorescent signals, rigid 2-component design

List Price:   $137.00
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Hard-Shell Full-Height 96-Well Semi-Skirted PCR Plates With Bar Codes

HSS-9901
Pkg of 25, clear shell/clear well 3-side (Row H, Column 1, and Column 12) bar-coded PCR plate to fit most instruments, rigid 2-component design

List Price:   $131.00
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Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
03526 Instruction Manual, Frame-Seal Incubation Chambers Click to download
03521 Multiplate 96- and 48-Well Unskirted PCR Plates Product Insert, Rev E Click to download
06366 Instruction Manual, Hard-Shell 96-Well Thin-Wall PCR Plates, Rev C Click to download
5496 Hard-Shell PCR Plates Brochure, Rev D Click to download
10012853 Hard-Shell 96-Well PCR Plates Product Insert, Rev D Click to download