Gene Pulser MXcell™ Electroporation System

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Overview

Choice of Waveforms

A choice of exponential or square waveforms. Both exponential-decay and square-wave pulses have been used very effectively for both electroporation and electrofusion. The shape of the electroporation wave can have a significant effect on the transformation efficiency for different cell types. The Gene Pulser MXcell system generates both exponential and square waveforms, enabling you to choose the waveform and protocol that will work best for your cells.

Exponential-decay pulse

Exponential-decay pulse. When a capacitor, charged to a voltage V0, is discharged into cells, the voltage applied to the cells decreases over time in an exponential curve such that the voltage V at any given time t is given by V = V0e-(t/RC). In the special case where t = Τ, then V = V0/e. The value CR is known as the time constant of the voltage decay. The shorter the time constant the faster the decay.

Square-wave pulse

Square-wave pulse. Truncating the pulse from a capacitor after discharging it into the sample generates a square-wave pulse. The pulse length is the time the cells are subjected to the discharge. During the pulse, the voltage again decreases by an exponential decay so that at the end of the pulse the voltage is lower than at the beginning. This drop in voltage is called the pulse droop and it is measured as a percentage of the initial voltage.

Fewer Cells and Less Sample

Optimize Your Resources

Use fewer cells and less sample — siRNA or DNA — by defining your parameters. Optimizing your electroporation parameters will ensure that cell viability and transfection efficiency are at their highest levels.

GAPDH mRNA levels in Jurkat cells postelectroporation.

A siLentMer Dicer-substrate siRNA duplex (GAPDH) or a scrambled negative control (NC) was delivered using the Gene Pulser MXcell system and Gene Pulser electroporation buffer, or a competitor's electroporator and cell line-specific buffer kit. Gene knockdown was measured by qPCR.


A, GAPDH mRNA levels 4 hr postelectroporation.


B, GAPDH mRNA levels 24 hr postelectroporation.

After 4 hr, >88% knockdown was obtained using the Gene Pulser MXcell system and Gene Pulser electroporation buffer, compared to only 31% knockdown using a competitor's system.

Silencing in Neuroblastoma Cells

Gene silencing in SK-N-SH cells electroporated with either a siLentMer GAPDH siRNA or a siLentMer nonspecific siRNA assessed using real-time RT-qPCR. Cells (3 x 106/ml) were electroporated (20 ms square-wave pulse of 200 V and 2,000 µF) with 50 nM (A) or 100 nM (B) siRNA. Nonspecific siRNA (); GAPDH siRNA (). C, over 90% gene silencing was obtained in cells treated with 50 or 100 nM GAPDH siRNA () compared to cells treated with the corresponding concentration of nonspecific siRNA () (negative control). RFU, relative fluorescence units.

Gene Pulser MXcell Electroporation System
Silencing in Jurkat Cells

Time course of GAPDH gene silencing in Jurkat cells. Values were normalized to scrambled negative control (lime green); silencing of 88% of gene expression was observed in cells transfected with GAPDH siRNA (dark green).

Delivery of siRNA

siRNA delivery into neutrophils using a range of electroporation conditions. Neutrophils were electroporated using the indicated conditions in the presence of siRNA conjugated to Alexa Fluor 546, then imaged by fluorescence microscopy. MFI was used as an index of siRNA delivery into the cells. Under conditions below 300 V, transfection efficiency increased when capacitance was increased. RFU, relative fluorescence units.

Transfection Protocol Library


The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. browse protocols to view our library and find your starting point.

Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser electroporator, and the Helios® gene gun and PDS-1000/He biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based transfection reagents — TransFectin lipid reagent for delivery of plasmid DNA and siLentFect lipid reagent for siRNA delivery.

Discontinued Product
The Gene Pulser MXcell electroporation system is no longer available. However, current users can order electroporation plates and cuvettes for their transfection applications.

Existing Gene Pulser MXcell systems can be used to electroporate as few as 105 cells by using a 96-well plate or as many as 106 to 107 cells in a 12-well plate or in a cuvette.

 

Alternatives to the Gene Pulser MXcell system for meeting your transfection needs include:

  • The Gene Pulser Xcell™ systems — modular electroporation systems for transfecting every cell type. They include a main unit, a ShockPod™ cuvette chamber, and your choice of accessory modules: the capacitance extender (CE) module and the pulse controller (PC) module
  • Lipid transfection reagents — provide optimal transfection efficiencies for nucleic acid delivery into mammalian cells. General use and RNAi-optimized transfection reagents are available
Gene Pulser Xcell™ Total SystemIncludes main unit, CE module, PC module, and ShockPod™ cuvette chamber  
Waveform Exponential or square
Voltage, V 10–3,000
Capacitance, µF 10–500 V: 25–3,275 µF in 25 µF increments; 500–3,000 V: 10, 25, 50 µF
Resistance (parallel), Ω 50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance, Ω 20 Ω minimum at 10–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing 10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval; 500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm Main unit: 31 x 30 x 14
CE module: 31 x 28 x 9
PC module: 31 x 28 x 5
Weight, kg Main unit: 6.6
CE module: 3.1
PC module: 1.9
Gene Pulser Xcell Eukaryotic System Includes main unit, CE module, and ShockPod cuvette chamber  
Waveform Exponential or square
Voltage, V 10–3,000
Capacitance, µF 10–500 V: 25–3,275 µF in 25 µF increments; 500–3,000 V: 10, 25, 50 µF
Sample resistance, Ω 20 Ω minimum at 10–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing 10–500 V: 0.05–10 ms in 0.05 ms increments, 10–100 ms pulse in 1 ms increments, 1–10 pulses, 0.1–10 sec interval; 500–3,000 V: 0.05–5 ms in 0.05 ms increments, 1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm Main unit: 31 x 30 x 14
CE module: 31 x 28 x 9
Weight, kg Main unit: 6.6
CE module: 3.1
Gene Pulser Xcell Microbial System Includes main unit, PC module, and ShockPod cuvette chamber  
Waveform Exponential or square
Voltage, V 200–3,000
Capacitance, µF 10, 25, 50
Resistance (parallel), Ω 50–1,000 Ω in 50 Ω increments, plus infinity
Sample resistance, Ω 20 Ω minimum at 200–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing 0.05–5 ms pulse duration in 0.05 ms increments,
1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm Main unit: 31 x 30 x 14
PC module: 31 x 28 x 5
Weight, kg Main unit: 6.6
PC module: 1.9
Gene Pulser Xcell Main Unit  
Waveform Exponential or square
Voltage, V 200–3,000
Capacitance, µF 10, 25, 50
Sample resistance 20 Ω minimum at 200–2,500 V; 600 Ω minimum at 2,500–3,000 V
Square-wave timing 0.05–5 ms pulse duration in 0.05 ms increments,1–2 pulses, 5 sec minimum interval
Dimensions (W x D x H), cm 31 x 30 x 14
Weight, kg 6.6
Number Description Options
5700 Instruction Manual, Gene Pulser MXcell Electroporation Guide, Rev A Click to download
10010739 Instruction Manual, Gene Pulser MXcell Electroporation System, Rev B Click to download [ Add to Cart (Free) ]
10010740 Instruction Manual, Gene Pulser MXcell Electroporation System Quick Guide, Rev A Click to download [ Add to Cart (Free) ]
10010741 Instruction Manual, Gene Pulser MXcell Electroporation System Pre Set Protocols, Rev A Click to download [ Add to Cart (Free) ]
10010742 Instruction Manual, Gene Pulser MXcell Electroporation System Plate, Rev A Click to download
5760A Gene Pulser MXCell Folder (includes bulletins 5634, 10015722, 5582, 5598, 5596, 5904, 5935, 5942, 5603, 5622, 5641, 5686, 5687, 5720, 5704, 5733, 5778, 5823, 5858) [ Add to Cart (Free) ]
5598 Gene Pulser MXcell Electroporation System Flier, Rev B Click to download
5634 Gene Pulser MXcell Electroporation System Brochure, Rev A Click to download [ Add to Cart (Free) ]
5858 The Gene Pulser MXcell Electroporation System Provides Reproducible Results in Electroporation Plates and Cuvettes With the Same Protocol, Rev A Click to download
5641 The Gene Pulser MXcell Electroporation System Delivers Consistent Results Required for Optimizing Delivery Protocols, Rev A Click to download
5603 Optimization of Electroporation Using Gene Pulser Electroporation Buffer and the Gene Pulser MXcell Electroporation System, Rev A Click to download
5622 Optimization of Electroporation Conditions With the Gene Pulser MXcell Electroporation System, Rev A Click to download
5686 Optimization of Electroporation Conditions for Jurkat Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5687 Transfection of Mammalian Cells Using Preset Protocols on the Gene Pulser MXcell Electroporation System, Rev A Click to download
5720 Transfection of Neuroblastoma Cell Lines Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5704 Electroporation Conditions for Chinese Hamster Ovary Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5733 Transfection of Chinese Hamster Ovary-Derived DG44 Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5774 Delivery of siRNA by Electroporation Into Primary Human Neutrophils Using the Gene Pulser MXcell System, Rev A Click to download
5778 Electroporation Parameters for Transfection of HL-60 Leukocytic Cell Line With siRNA Using the Gene Pulser MXcell System, Rev B Click to download
5823 Electroporation of Primary Murine Mast Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5842 Optimization of Electroporation Conditions for Two Different Burkitt Lymphoma Cell Lines Using the Gene Pulser MXcell System, Rev B Click to download
5684 Poster: Optimization Is Essential to Increase Transfection Efficiency and Cell Viability in Primary and Difficult-to-Transfect Cell Lines, Rev A Click to download
0108 Simple and Rapid Optimization With Maximum Transfection Efficiency, Rev A Click to download
5860 Analysis of IL-4 Dependent Gene Expression in Namalwa Cells by siRNA Transfection: An Example of Pathway Analysis Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5904 Transfection of Mouse and Human Embryonic Stem Cells by Electroporation Using the Gene Pulser MXcell System, Rev A Click to download
5935 Electroporation of Smooth Muscle Cells Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5942 Delivery of siRNA into B-Cell Lymphomas Using the Gene Pulser MXcell Electroporation System, Rev A Click to download
5924 Stem Cell Basics for Life Science Researchers Brochure, Rev A Click to download [ Add to Cart (Free) ]