Rotofor® and Mini Rotofor Cells

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Overview

Specifications
Specification Mini Rotofor Cell Rotofor Cell
Number of fractions 20 20
Focusing chamber inner diameter 19 mm 30 mm
Sample volume 18 ml 35–60 ml
Fraction volume 0.7 ml 1.75–3 ml
Sample load Microgram to milligram Milligram to gram
Power conditions required 3,000 V with 12 W constant 3,000 V with 15 W constant
Cooling Cooling finger (requires external recirculating water chiller); temperature flexible
Dimensions (W x D x H) 16.5 x 45.7 x 22.8 cm 16.5 x 45.7 x 22.8 cm
Weight 9 kg (20 lb) 9 kg (20 lb)

The Rotofor and Mini Rotofor cells use liquid-phase IEF to separate proteins into 20 fractions in free solution. As an initial purification step, these cells concentrate proteins and eliminate those outside of a pI range of interest. As a final purification step, they eliminate protein contaminants that may be difficult to remove by other means.

Because it can process up to gram quantities of protein and effectively concentrates proteins into discrete zones based on pI, the Rotofor is a proven and effective method for the enrichment of low-abundance proteins. In a single run, the Rotofor cell concentrates samples up to 20-fold; selected fractions may also be pooled and refractionated on either the Mini Rotofor cell or the MicroRotofor™ cell to yield even greater purification. In 2-D electrophoresis applications, protein fractionation by pI improves downstream sample loading and separation on narrow- and micro-range IPG strips by eliminating proteins outside the pI region of interest.

Accessory equipment required to run the Rotofor and Mini Rotofor cells includes a 3,000 V power supply such as the PowerPac™ HV power supply, a recirculating water chiller, and house vacuum. Bio-Lyte® ampholytes are required to form the internal pH gradient.

Related Products

Different configurations of the Rotofor system are available to accommodate different sample loads. The Rotofor, Mini Rotofor, and MicroRotofor cells all share the following features:

  • Interchangeable cylindrical focusing chambers, which enable refractionation of selected fractions for even greater purification
  • Maintenance of biological activity
  • Customizable pH gradients — wide or narrow linear pH gradients can be generated using Bio-Lyte ampholytes
  • Focusing and enrichment of a protein of interest into 1 or 2 fractions in a single step

 

  Rotofor Cell Mini Rotofor Cell MicroRotofor Cell
Number of fractions 20 20 10
Sample volume 35–60 ml 18 ml 2.5 ml
Fraction volume 1.75–3 ml 0.7 ml 200–250 µl
Sample load Milligram to gram Microgram to milligram Up to to 1 mg
Rotofor Purification System

170-2986
100/120 V, preparative IEF cell system, sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit

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Rotofor Purification System

170-2987
220/240 V, preparative IEF cell system, sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit

List Price:   $6,953.00
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Standard Rotofor Cell

170-2950
100/120 V, preparative IEF cell with 60 ml focusing chamber, sample volume 35–60 ml, includes starter kit

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Standard Rotofor Cell

170-2951
220/220 V, preparative IEF cell with 60 ml focusing chamber, sample volume 35–60 ml, includes starter kit

List Price:   $6,231.00
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Mini Rotofor Cell

170-2988
100/120 V, preparative IEF cell, sample volume 18 ml, includes 18 ml focusing chamber, starter kit

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Mini Rotofor Cell

170-2989
220/240 V, preparative IEF cell, sample volume 18 ml, includes 18 ml focusing chamber, starter kit

List Price:   $6,160.00
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Rotofor Purification System

170-2914
100/120 V, preparative IEF cell system and PowerPac™ HV power supply (#164-5056), sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit

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Rotofor Purification System with PowerPac HV Power Supply

170-2906
220/240 V, preparative IEF cell system and power supply (#164-5056), sample volume 35–60 ml, includes 60 ml and 18 ml focusing chambers, starter kit

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Number Description Options
3160 Rotofor Cell Bio-Rad Literature Click to download
3152 Rotofor Cell Public Domain Literature Click to download
1517 Rotofor Fractionation of Intestinal Brush Border Membrane Proteins, Rev B Click to download
3131 The Rotofor System As a Prefractionation Device Used Prior to Electrophoresis, Rev A Click to download
2219 Rotofor and Mini Rotofor Assembly Guide Click to download
2859 Combination of 2-D Gel and Liquid-Phase Electrophoretic Separations As Proteomic Tools in Neuroscience, Rev A Click to download
M1702954 Instruction Manual, Rotofor Preparative IEF Cell Heat Exchanger O-Ring Kit, Rev C Click to download
LIT603 Instruction Manual, Rotofor System Starter Kit Click to download
M1702950 Instruction Manual, Rotofor and Mini Rotofor, Rev E Click to download
1903 Rotofor System Brochure, Rev C Click to download