MicroRotofor™ Cell

Description

Description

The MicroRotofor cell uses liquid-phase IEF to fractionate small volumes (2.5 ml) of proteins in free solution by their pI. The MicroRotofor cell can be used to reduce the complexity of samples prior to any protein analysis technique (including mass spectrometry and 2-D electrophoresis). In 2-D electrophoresis applications, protein fractionation by pI improves downstream sample loading and separation on narrow- and micro-range IPG strips. Whether used alone or as part of a broader separation or purification scheme, the MicroRotofor cell offers rapid, simple, and effective fractionation and enrichment of proteins from complex protein mixtures.

The MicroRotofor cell is easy to set up and offers:

  • Unique liquid-phase fractionation by pI, eliminating proteins outside a pI range of interest
  • Up to tenfold concentrations of proteins of interest
  • Customizable pH gradients — wide or narrow linear pH gradients can be generated using Bio-Lyte® ampholytes
  • Rapid recovery of proteins focused into 10 liquid fractions
  • Integrated temperature regulation to improve reproducibility and to preserve protein structure and function for native separations
  • Preassembled disposable focusing chambers

Accessory equipment required to run the MicroRotofor cell includes a 1,000 V power supply such as the PowerPac™ HV power supply (which is capable of running at 1 W constant power) and house vacuum. Bio-Lyte ampholytes are required to form the internal pH gradient.

Related Products

Different configurations of the Rotofor system are available to accommodate different sample loads. The Rotofor®, Mini Rotofor, and MicroRotofor cells all share the following features:

  • Interchangeable cylindrical focusing chambers, which enable refractionation of selected fractions for even greater purification
  • Maintenance of biological activity
  • Customizable pH gradients — wide or narrow linear pH gradients can be generated using Bio-Lyte ampholytes
  • Focusing and enrichment of a protein of interest into 1 or 2 fractions in a single step

 

  Rotofor Cell Mini Rotofor Cell MicroRotofor Cell
Number of fractions 20 20 10
Sample volume 35–60 ml 18 ml 2.5 ml
Fraction volume 1.75–3 ml 0.7 ml 200–250 µl
Sample load Milligram to gram Microgram to milligram Up to to 1 mg

 

MicroRotofor cell lysis kits provide convenient cell lysis and protein extraction protocols to generate total protein samples that are ready for fractionation with a MicroRotofor IEF cell or other downstream applications such as SDS-PAGE and 2-D gel electrophoresis. Kits are available for mammalian, bacterial, plant, and yeast cells.

Ordering

Ordering

items

Use the filters below to refine results!

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1702801
220/240 V, preparative IEF device, with accessories (high voltage power supply required, #164-5056 sold separately)
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1702802
100/120 V, preparative IEF device with accessories (#170-2800) with high voltage power supply (#164-5056)
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1702803
220/240 V, preparative IEF device with accessories (#170-2800) with high voltage power supply (#164-5052)

Accessories

Accessories

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7318140
Discontinued Item
Replaced by Standard BioLogic™ LP Low-Pressure Chromatography System (7318300)
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7318122
100/120 V, carousel-type fraction collector, 80 tube capacity, for use in chromatographic separations
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1645070
100–120/220–240 V, power supply for a broad range of electrophoresis and blotting applications, includes power cord
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(Discontinued) Sealing Tape
1702960
Pkg of 1 roll, 1" x 36 yards, sealing tape, for use with Rotofor and mini Rotofor cell
(Discontinued) MicroRotofor™ Lid
1702855
Pkg of 1, replacement part, for use with MicroRotofor cell
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1702850
Pkg of 1, replacement part, includes alignment station, needle assembly, needle holder, for use with MicroRotofor cell
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1702832
Pkg of 1, plastic riveted block used to prepare tape for sealing the focusing chamber of the MicroRotofor cell

Documents

Documents

Number Description Options
5451
First-Dimension Separation With the MicroRotofor Cell Prior to SDS-PAGE and LC-MS/MS Analysis, Rev A
5349
Fractionation by Liquid-Based Isoelectric Focusing With the MicroRotofor Cell: Improving the Resolution of Low-Abundance Proteins on 2-D Gels (Poster), Rev A
5344
Fractionation by Liquid-Phase Isoelectric Focusing in the MicroRotofor Cell: Improved Detection of Low-Abundance Proteins, Rev A
10004399
Instruction Manual, MicroRotofor Cell Starter Kit Manual, Rev A
10008759
MicroRotofor Cell Tutorial DVD
5294
MicroRotofor Cell Product Information Sheet