The Fish DNA Barcoding Kit is an advanced PCR kit that allows students to determine the species of a fish sample based on its DNA sequence of the cytochrome c oxidase I gene.
With this kit, students are able to:
- Perform DNA extraction, PCR amplification, and gel electrophoresis
- Use sequencing and bioinformatics to determine fish species
- Contribute to the global initiatives to barcode life
Species are traditionally identified by taxonomists who need to have in-depth knowledge of certain species characteristics and environments in order to categorize them. The power of DNA research now allows us to add a level of identification based on the sequencing of a conserved gene. The subtle changes in sequences result in species-specific identification or genetic barcodes. This information is critical in helping to assess biodiversity and conservation efforts, as well as commercially, where food marketplaces can be regulated.
This kit provides sufficient materials for processing up to 16 fish and control samples (8 student work stations, 2 samples per workstation, 2–4 students per workstation).
The DNA Barcoding Sequencing Module allows you to order sequencing services now and use them when you’re ready. Sample shipping to a validated sequencing facility is included with this module! PCR cleanup and sequencing are included for up to nine samples per sequencing module.
Download the Fish DNA Barcoding Quiz Game, a fun activity to get your students excited about the Fish DNA Barcoding lab.
Use this fun Microplate Art Pipetting Activity to help your students practice accurate pipetting skills while creating art.
Download this Fish Flashcard activity for a fun way to introduce the Fish DNA Barcoding Laboratory to your class.