Experion Pro260 Starter Kit

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Overview

Experion System
Experion System Training
Experion™ Pro260 Ladder Used for Sizing and Alignment
 

 

Note the flat baseline and well-resolved peaks. All identified peaks are numbered, and the lower and upper markers are indicated. The 260 kD protein in the Pro260 ladder is labeled as the upper marker by Experion software.

Carbonic Anhydrase Electropherogram

Electropherogram of bovine carbonic anhydrase illustrating the relative positions of the lower alignment marker, system peaks, carbonic anhydrase, and upper marker.

Pipet Tips Recommended for Experion Chip Loading

(Narrow-bore tips such as A and B are recommended. Tips C,D,E not recommended.)


Filter tips are always recommended for RNA work.

Examples of recommended pipet tips for loading chips:

For RNA or DNA work:

  • Rainin L-10F
  • Rainin SR-L10F
  • Rainin GP-L10F
  • VWR 87001-688

For protein work:

  • Rainin GP-L10F
  • Rainin GP-10G
  • Rainin GP-10F
  • Rainin GP-10S
  • Rainin GP-10
  • VWR 87001-688

 

Using the Experion Software for Protein Analysis Webinar

Field Application Scientist Sean Taylor demonstrates the various features of the Experion software for protein data analysis. Duration: 25 min.

Click here to view webinar

The Experion™ Pro260 starter kit is intended for new or experienced users of the Experion automated electrophoresis system. This kit contains all materials necessary to get started on the Experion system, allowing the user to practice techniques and analyze data or validate the system using a known protein standard (bovine IgG). Get started or practice with step-by-step instructions and tips for successful microfluidic separations using the provided standard before moving onto your own samples. An Experion RNA starter kit is also available for studying RNA samples (StdSens RNA starter kit).

The Experion protein starter kit includes all the necessary consumables to illustrate the utility of the Experion system in protein applications. This kit provides information on:

  • How best to prepare and load a protein chip
  • Protein quantitation and sizing using a known standard
  • Creating and running a standard curve for protein analysis
  • Tips and common mistakes

The Pro260 protein starter kit contains:

  • Experion Pro260 reagents
  • Experion Pro260 chips, 3
  • Narrow-bore polypropylene pipet tips
  • Polypropylene microcentrifuge tubes
  • 0.2 µ filtered water
  • DTT reducing agent
  • Spin filters
  • Electrode cleaner
  • Cleaning swabs
  • Cleaning chips
  • Bovine IgG control sample
  • Detailed instruction manual

The Experion automated electrophoresis system can also be used for a number of upstream and downstream workflows in other nucleic acid and protein applications.

Component Storage Condition 
Bovine γ-globulin (BGG) standard (2.0 mg/ml), 2 ml 4°C 
Dithiothreitol (DTT) 4°C  
DEPC-treated water 1, 50 ml Room temperature 
Experion™ electrode cleaner, 25 ml Room temperature  
Experion cleaning chip, 1 Room temperature   
Cleaning swabs, 25 Room temperature    
Narrow-bore 20 µl pipet tips, 96 (1 rack) Room temperature     
0.65 ml RNase-free microcentrifuge tubes, 50 Room temperature      
Experion Pro260 chips, 3 Room temperature       
Reagents for analysis  
Pro260 gel, 2 vials, 520 µl 4°C   
Pro260 ladder, 60 µl 4°C    
Pro260 sample buffer, 400 µl 4°C     
Pro260 stain, 45 µl 4°C      
Spin filters 4°C       
Experion training video: Chip Loading (DVD)
Instructions
Experion Pro260 Starter Kit

700-7110
Starter kit for Experion protein analysis, includes 3 Pro260 chips, reagents, spin filters, IgG protein standard, DTT, cleaning supplies, tips, tubes, ultrapure water

List Price:   $754.00
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Experion Protein Ladder

700-7256
60 μl, protein size standards, replacement component of Experion Pro260 analysis kits

List Price:   $92.00
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Experion Pro260 Chips

700-7151
Pkg of 10 plus 1 cleaning chip, microfluidic chips for protein analysis with the Experion automated electrophoresis system

List Price:   $387.00
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Quick Start Bovine γ-Globulin Standard

500-0208
5 x 2 ml vials, 2 mg/ml, prediluted standard for use with Quick Start Bradford protein assay kits

List Price:   $92.00
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ReadyPrep Proteomics Grade Water

163-2091
500 ml, ultrapure water for use with ReadyPrep fractionation and general purpose cleanup kits

List Price:   $49.00
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Experion Cleaning Chips

700-7251
Pkg of 10, cleaning chip for use with the Experion electrophoresis station (#700-7010)

List Price:   $157.00
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Experion Electrode Cleaner

700-7252
250 ml, for use with Experion cleaning chips (#700-7251)

List Price:   $60.00
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Experion Spin Filters

700-7254
Pkg of 10, spin filter, for use with Experion analysis kits

List Price:   $74.00
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Cleaning Swabs

700-7264
Pkg of 25, swab for use with any Experion analysis kit

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Number Description Options
BR123 BioRadiations Magazine, Number 123 Click to download [ Add to Cart (Free) ]
BR125 BioRadiations Magazine, Number 125 Click to download [ Add to Cart (Free) ]
3140 Experion Automated Electrophoresis System Brochure, Rev F Click to download [ Add to Cart (Free) ]
3169 Experion Pro260 Analysis Kit PIS, Rev E Click to download [ Add to Cart (Free) ]
3171 Experion Software PIS, Rev C Click to download [ Add to Cart (Free) ]
3174A Experion Folder (includes bulletins 3140, 3169, 3170, 3171, and 3184) [ Add to Cart (Free) ]
3176 Monitoring the Expression, Purification, and Processing of GST-Tagged Proteins Using the Experion Automated Electrophoresis System, Rev A Click to download
3184 Experion Video Tour CD [ Add to Cart (Free) ]
3186 Experion Automated Electrophoresis System Flier, Rev A Click to download
5253 Experion System Performance Comparison, Rev A Click to download
5285 Bio-Rad Applies Microfluidics to Automate Gel Electrophoresis, Rev C Click to download [ Add to Cart (Free) ]
5299 Performance Comparison of the Experion Automated Electrophoresis System and SDS-PAGE for Protein Analysis, Rev B Click to download [ Add to Cart (Free) ]
5302 Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A Click to download
5328 Experion Automated Electrophoresis System and the Experion Pro260 Analysis Kit: Accurate and Reproducible Protein Sizing and Quantitation in the Presence of High Salt Concentrations, Rev A Click to download
5350 Monitoring the Expression, Processing, and Purification of GST-Tagged Proteins Using the Experion Automated Electrophoresis System (Poster Version), Rev A Click to download
5351 Rapid and Accurate Protein Sizing, Quantitation, and Analysis Using the Experion Automated Electrophoresis System and the Experion Pro260 Analysis Kit (Poster), Rev A Click to download
5355 Rapid Screening of Mini Rotofor Fractions Using the Experion Automated Electrophoresis System (Poster), Rev A Click to download
5423 Protein Quantitation: a Comparative Analysis Using the Experion Automated Electrophoresis System, Bradford and Modified Lowry Assays, and SDS-PAGE, Rev A Click to download
5453 Application of the Experion Automated Electrophoresis System to Glycoprotein Visualization and Analysis, Rev A Click to download
5460 Biomarker Discovery Workflow Brochure, Rev B Click to download
5501 Rapid, Efficient Purification and Evaluation of His-Tagged Proteins, Rev A Click to download
5506 Monitoring Development of Chromatographic Methods With the Experion Automated Electrophoresis System, Rev B Click to download [ Add to Cart (Free) ]
5514 Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A Click to download [ Add to Cart (Free) ]
5712 The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A Click to download [ Add to Cart (Free) ]
5732 Experion Pro260 and RNA StdSens Starter Kits PIS, Rev B Click to download [ Add to Cart (Free) ]
5770 Affinity Purification of Native, Tag-Free Cystathionine Beta-Lysate (MetC) From Shewanella oneidensis Using the Profinity eXact Fusion-Tag and Profinia Protein Purification Systems, Rev A Click to download [ Add to Cart (Free) ]
5783 Experion Automated Electrophoresis System Flier, Rev A Click to download [ Add to Cart (Free) ]
5784 Comparison of Protein Quantitation Methods Using the Experion Automated Electrophoresis System Click to download
5868 Performance Comparison of Two LabChip Microfluidic Platforms for Protein, DNA, and RNA Analyses, Rev A Click to download
5926 Modular Protein Purification, Rev A Click to download [ Add to Cart (Free) ]
10000975 Instruction Manual, Experion Pro260 Analysis Kit, Rev C Click to download
10001312 Instruction Manual, Experion Automated Electrophoresis System, Rev C Click to download
10004490 Quick Guide, Experion Pro260 Analysis Kit, Rev C Click to download
10005250 Instruction Manual, IQ/OQ Protocols and Instuctions, Experion Electrophoresis Station, Rev D Click to download
10005581 Instruction Manual, Experion Security Edition Software Setup Guide, Rev C Click to download
10005740 Quick Installation Guide, Experion System Software Windows XP, Rev A Click to download
10010510 Instruction Manual, Experion Pro260 Starter Kit, Rev A Click to download
10010511 Instruction Manual, Experion RNA StdSens Starter Kit, Rev A Click to download
10014627 Quick Installation Guide, Experion System Software Windows VISTA, Rev A Click to download