ELISA Substrate Kits

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Overview

Comparison of ELISA Substrate Systems
  AP HRP
 
ELISA Type pNPP ABTS TMB
Qualitative +++ ++++ ++++
Stop reagent Sodium hydroxide Oxalic acid Sulfuric acid
Color Yellow Blue-green Yellow
Detection wavelength 405 nm 415 nm 450 nm
Quantitative (Endpoint) ++++ +++ +++
Stop reagent Sodium hydroxide Oxalic acid Sulfuric acid
Color Yellow Blue-green Yellow
Detection wavelength 405 nm 415 nm 450 nm
Quantitative (Kinetic) ++ +++ ++++
Color Yellow Blue-green Blue
Detection wavelength 405 nm 415 nm 655 nm
Substrate performance: ++++ excellent; +++, good; ++, fair.

Bio-Rad offers ELISA substrate kits suitable for use in all microtitration enzyme immunoassays. The Bio-Rad AP substrate kit contains easy-to-use p-nitrophenyl phosphate tablets and dilution buffer concentrate to prepare 500 ml of working solution. Benefits of the AP system include an extremely linear reaction rate and low background values for accurate quantitation by ELISA.

Two convenient substrate systems are available for detection of HRP-labeled antibodies. Both the ABTS (2,2'-azino-di(3-ethylbenzthiazoline)-6-sulfonic acid) peroxidase substrate kit and the TMB (3,3',5,5'-tetramethylbenzidine) peroxidase substrate kit contain liquid reagents that are mixed just prior to use. No weighing or dissolving of hazardous chemicals is necessary. The single-component TMB peroxidase EIA substrate kit makes microplate assays convenient and fast.

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AP Substrate Kit

1721063
Alkaline phosphatase substrates, for immunodetection, includes 100 pNPP tablets, 100 ml 5x concentrate diethanolamine buffer, for 500 ml

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$283.00
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HRP Substrate Kit

1721064
Horseradish peroxidase substrates, for immunodetection, includes 180 ml ABTS, 20 ml hydrogen peroxide, detection wavelength 415 nm

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$219.00
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TMB Peroxidase EIA Substrate Kit

1721066
Peroxidase substrates, for immunodetection, kit contains 180 ml TMB solution and 20 ml hydrogen peroxide, detection wavelength 450 nm

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$225.00
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TMB Peroxidase EIA Substrate Kit

1721067
For detection of HRP-labeled antibodies, kit contains 900 ml TMB solution and 100 ml hydrogen peroxide, detection wavelength 450 nm

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$605.00
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Single-Component TMB Peroxidase EIA Substrate Kit

1721068
Single-component, contains 100 ml TMB solution, detection wavelength 450 nm

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$102.00
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Single-Component TMB Peroxidase ELISA Substrate Kit

1721072
Single-component, contains 1 L TMB solution, detection wavelength 450 nm

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$889.00
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Number Description Options
LIT172 Instruction Manual, Alkaline Phosphatase Substrate Kit Click to download
LIT445 Instruction Manual, Peroxidase Substrate Kit, Rev D Click to download
LIT176 Instruction Manual, TMB Peroxidase EIA Substrate Kit, 1721066, Rev C Click to download
LIT572 Instruction Manual, Single Component TMB Peroxidase EIA Substrate Kit Click to download
Lit452 Instruction Manual, Rabbit Anti-Goat IgG-Horseradish Peroxidase Conjugate, Rev C Click to download