ELISA Enzyme-Antibody Conjugates

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Overview

Comparison of ELISA Substrate Systems
  AP HRP
 
ELISA Type pNPP ABTS TMB
Qualitative +++ ++++ ++++
Stop reagent Sodium hydroxide Oxalic acid Sulfuric acid
Color Yellow Blue-green Yellow
Detection wavelength 405 nm 415 nm 450 nm
Quantitative (Endpoint) ++++ +++ +++
Stop reagent Sodium hydroxide Oxalic acid Sulfuric acid
Color Yellow Blue-green Yellow
Detection wavelength 405 nm 415 nm 450 nm
Quantitative (Kinetic) ++ +++ ++++
Color Yellow Blue-green Blue
Detection wavelength 405 nm 415 nm 655 nm
Substrate performance: ++++ excellent; +++, good; ++, fair.

Bio-Rad offers enzyme-antibody conjugates suitable for use in all microtitration enzyme immunoassays. Antibodies conjugated to HRP or AP are available. Affinity purification procedures have been used to isolate specific antibodies and to remove nonspecific antibodies and other serum proteins that can interfere in assay procedures.

Goat Anti-Mouse IgG (H+L)-HRP Conjugate

172-1011
2 ml, EIA-grade, enzyme-antibody conjugate

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Rabbit Anti-Sheep IgG (H+L)-HRP Conjugate

172-1017
1 ml, EIA-grade, enzyme-antibody conjugate

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Goat Anti-Rabbit IgG (H+L)-HRP Conjugate

172-1019
1 ml, EIA-grade, enzyme-antibody conjugate

List Price:   $115.00
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Goat Anti-Human IgG (gamma)-HRP Conjugate

172-1033
1 ml, EIA-grade, enzyme-antibody conjugate

List Price:   $261.00
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Rabbit Anti-Goat IgG (H+L)-HRP Conjugate

172-1034
1 ml, EIA-grade, enzyme-antibody conjugate

List Price:   $212.00
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Rabbit Anti-Goat IgG (H+L)-AP Conjugate

172-1037
1 ml, EIA-grade, enzyme-antibody conjugate

List Price:   $270.00
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Number Description Options
M1721033 Instruction Manual, EIA Grade Goat Anti-Human IgG-Horseradish Peroxidase Conjugate Click to download
M1721004 Instruction Manual, EIA Grade Goat Anti-Human IgG (Bovine Serum Adsorbed)-AP Click to download
LIT431 Instruction Manual, EIA Grade Affinity Purified Goat Anti-Rabbit IgG (H + L) Horseradish Peroxidase Conjugate Click to download
Lit429 Instruction Manual, EIA Grade Affinity Purified Goat Anti-Mouse IgG (H+L) Horseradish Peroxidase Conjugate, Rev C Click to download
Lit432 Instruction Manual, EIA Grade Affinity Purified Goat Anti-Mouse (H+L) Alkaline Phosphatase, Rev A Click to download
LIT435 Instruction Manual, EIA Grade Goat Anti-Rabbit HRP Conjugate Click to download [ Add to Cart (Free) ]
9381 Instruction Manual, EIA Grade Goat Anti-Rabbit-Alkaline Phosphatase Conjugate Click to download
LIT434 Instruction Manual, EIA Grade Protein A-HRP Conjugate, catalog #172-1018 Click to download
LIT445 Instruction Manual, Peroxidase Substrate Kit, Rev D Click to download [ Add to Cart (Free) ]
LIT176 Instruction Manual, TMB Peroxidase EIA Substrate Kit, 1721066, Rev C Click to download
LIT572 Instruction Manual, Single Component TMB Peroxidase EIA Substrate Kit Click to download [ Add to Cart (Free) ]
Lit452 Instruction Manual, Rabbit Anti-Goat IgG-Horseradish Peroxidase Conjugate, Rev C Click to download