Crime Scene Investigator PCR Basics™ Real-Time PCR Starter Kit

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Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies
Crime Scene Investigator PCR Basics Kit 1
iQ SYBR® Green Supermix, 5 ml 1
Agarose powder, 25 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast DNA stain, 500x, 100 ml 1
Sterile water, 500 ml 1
PCR tube strips 120
Optical flat caps 120
Curriculum, including Teacher's Guide and Student Manual 1
Required Accessories Not Included in Kits:
Horizontal gel electrophoresis chambers 4–8
Adjustable micropipets
   2–20 μl 1–8
   20–200 μl 1
   100–1,000 μl 1
Pipet tips, aerosol barrier  
   2–1,000 µl, Xcluda B, D, and E  
Power supplies 2–4
Real-Time thermal cycler 1
Microcentrifuges 1–4
Recommended (Optional) Accessories:
Rocking platform  
Microwave oven  
Real-Time PCR Flowchart
Real-Time PCR Flowchart
Key Kit Features
  • Quantitate DNA
  • Discover key differences between conventional and real-time PCR analysis
  • Analyze and evaluate real-time PCR results
  • Perform melt-curve analysis
  • Determine the accuracy and reliability of pipetting techniques
  • Learn the molecular basis of DNA amplification reactions using real-time PCR detection systems
Conventional PCR Animation

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Sample Real-Time PCR Results
Sample Real-Time PCR Results

Sample Real-Time PCR Results.

Curriculum Fit
Curriculum Fit

The Crime Scene Investigator PCR Basics™ Real-Time PCR Starter Kit is a tool for teaching students the principles of PCR and its use in forensic DNA analysis. This kit is a good starting point for teaching real-time PCR to novices to familiarize them with this valuable technique. Additionally, DNA fingerprints can still be investigated using gel electrophoresis and melt-curve analysis, showing how real-time and conventional PCR can be complementary techniques.

Features and Benefits

With this kit, students are able to:

  • Quantitate DNA
  • Discover key differences between conventional and real-time PCR analysis
  • Analyze and evaluate real-time PCR results and perform melt-curve analysis
  • Determine the accuracy and reliability of pipetting techniques
  • Learn the molecular basis of DNA amplification reactions using real-time PCR detection systems

Applications and Uses

This kit can be extended to demonstrate real-time PCR in two different labs. Each may be performed separately or run in sequence for greater depth of understanding and increased hands-on experience.

Laboratory scenario 1 preserves the original intent of the Crime Scene Investigator PCR Basics Kit, allowing students to observe real-time PCR as the reactions progress and to amplify and compare suspect versus crime scene DNA samples using agarose gel electrophoresis.

Laboratory scenario 2 is more advanced and modifies the Crime Scene Investigator PCR Basics Kit protocol to include a DNA dilution series and an optional very fast PCR protocol. This lab allows for a rapid demonstration of the quantitative nature of real-time PCR and melt-curve analysis, and in doing so uses an entirely different experimental design than the core Crime Scene Investigator PCR Basics Kit.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies  
Crime Scene Investigator PCR Basics™ kit 1
iQ™ SYBR® Green Supermix, 5 ml 1
Agarose powder, 25 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Sterile water, 500 ml 1
PCR tube strips 120
Optical flat caps 120
Curriculum, including Teacher's Guide and Student Manual 1
Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit

166-2660EDU
Introductory real-time PCR classroom study kit, contains Crime Scene Investigator PCR Basics kit (#166-2600) and additional real-time reagents; education use only

List Price:   $871.00
EDU Price:   $696.80   What is this?
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Number Description Options
10002461 Instruction Manual, Crime Scene Investigator PCR Basics Kit, Biotechnology Explorer, Rev D Please login to download this document [ Add to Cart (Free) ]
M1662605 Crime Scene Investigator PCR Basics Kit: A Real-Time PCR Extension, Biotechnology Explorer, Application Note, Rev A Please login to download this document
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download