Crime Scene Investigator PCR Basics™ Real-Time PCR Starter Kit

Print
Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit

Overview

Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies
Crime Scene Investigator PCR Basics Kit 1
iQ SYBR® Green Supermix, 5 ml 1
Agarose powder, 25 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast DNA stain, 500x, 100 ml 1
Sterile water, 500 ml 1
PCR tube strips 120
Optical flat caps 120
Curriculum, including Teacher's Guide and Student Manual 1
Required Accessories Not Included in Kits:
Horizontal gel electrophoresis chambers 4–8
Adjustable micropipets
   2–20 μl 1–8
   20–200 μl 1
   100–1,000 μl 1
Pipet tips, aerosol barrier  
   2–1,000 µl, Xcluda B, D, and E  
Power supplies 2–4
Real-Time thermal cycler 1
Microcentrifuges 1–4
Recommended (Optional) Accessories:
Rocking platform  
Microwave oven  
Real-Time PCR Flowchart
Real-Time PCR Flowchart
Key Kit Features
  • Quantitate DNA
  • Discover key differences between conventional and real-time PCR analysis
  • Analyze and evaluate real-time PCR results
  • Perform melt-curve analysis
  • Determine the accuracy and reliability of pipetting techniques
  • Learn the molecular basis of DNA amplification reactions using real-time PCR detection systems
Conventional PCR Animation

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player

Sample Real-Time PCR Results
Sample Real-Time PCR Results

Sample Real-Time PCR Results.

Curriculum Fit
Curriculum Fit

The Crime Scene Investigator PCR Basics™ Real-Time PCR Starter Kit is a tool for teaching students the principles of PCR and its use in forensic DNA analysis. This kit is a good starting point for teaching real-time PCR to novices to familiarize them with this valuable technique. Additionally, DNA fingerprints can still be investigated using gel electrophoresis and melt-curve analysis, showing how real-time and conventional PCR can be complementary techniques.

Features and Benefits

With this kit, students are able to:

  • Quantitate DNA
  • Discover key differences between conventional and real-time PCR analysis
  • Analyze and evaluate real-time PCR results and perform melt-curve analysis
  • Determine the accuracy and reliability of pipetting techniques
  • Learn the molecular basis of DNA amplification reactions using real-time PCR detection systems

Applications and Uses

This kit can be extended to demonstrate real-time PCR in two different labs. Each may be performed separately or run in sequence for greater depth of understanding and increased hands-on experience.

Laboratory scenario 1 preserves the original intent of the Crime Scene Investigator PCR Basics Kit, allowing students to observe real-time PCR as the reactions progress and to amplify and compare suspect versus crime scene DNA samples using agarose gel electrophoresis.

Laboratory scenario 2 is more advanced and modifies the Crime Scene Investigator PCR Basics Kit protocol to include a DNA dilution series and an optional very fast PCR protocol. This lab allows for a rapid demonstration of the quantitative nature of real-time PCR and melt-curve analysis, and in doing so uses an entirely different experimental design than the core Crime Scene Investigator PCR Basics Kit.

More Information

  • Three-session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation), plus additional real-time reagents for further studies  
Crime Scene Investigator PCR Basics™ kit 1
iQ™ SYBR® Green Supermix, 5 ml 1
Agarose powder, 25 g 1
Electrophoresis buffer, 50x TAE, 100 ml 1
Fast Blast™ DNA stain, 500x, 100 ml 1
Sterile water, 500 ml 1
PCR tube strips 120
Optical flat caps 120
Curriculum, including Teacher's Guide and Student Manual 1

Account price failed to display. Please try reloading the page. Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at   or send an
email to 

Crime Scene Investigator PCR Basics Real-Time PCR Starter Kit

1662660EDU
Introductory real-time PCR classroom study kit, contains Crime Scene Investigator PCR Basics kit (#166-2600) and additional real-time reagents; education use only

List Price:
 
$871.00
Loading
Your Price:
 

Communication error

Reload Page

EDU Price:   $696.80   What is this?
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
10002461 Instruction Manual, Crime Scene Investigator PCR Basics Kit, Biotechnology Explorer, Rev D Please login to download this document [ Add to Cart (Free) ]
M1662605 Crime Scene Investigator PCR Basics Kit: A Real-Time PCR Extension, Biotechnology Explorer, Application Note, Rev A Please login to download this document
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download