Crime Scene Investigator PCR Basics™ Kit

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Crime Scene Investigator PCR Basics Kit

Overview

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Lab Preparation Checklist
Kit contains sufficient materials for 8 student workstations. (2–4 students per workstation)
Crime scene DNA sample, 250 µl 1
Suspect A DNA sample, 250 µl 1
Suspect B DNA sample, 250 µl 1
Suspect C DNA sample, 250 µl 1
Suspect D DNA sample, 250 µl 1
PCR master mix (Taq DNA polymerase, dNTPs, buffer) 1.2 ml
Primers (blue), 25 µl 1
Allele ladder, 200 µl 1
Orange G loading dye, 1 ml 1
PCR tubes, 0.2 ml 50
Capless PCR tube adaptors, 1.5 ml 50
Colored micro test tubes, 2.0 ml 72
Foam micro test tube holders 8
Curriculum, including Teacher's Guide, Student Manual, and graphic Quick Guide 1
Required Accessores Not Included in Kit:
Horizontal gel electrophoresis chambers 4–8
Adjustable micropipets
    2–20 µl 1–8
    20–200 µl 1
Pipet tips, aerosol barrier
    2–20 µl Xcluda B 1 box
    20–200 µl, Xcluda D 1 box
Microcentrifuges 1–4
Thermal cycler 1
Power supplies 2–4
Recommended (Optional) Accessories:
Rocking platform
Microwave oven
Crime Scene Investigator PCR Basics Flowchart
Crime Scene Investigator PCR Basics Flowchart
Key Kit Features
  • Perform real world DNA profiling
  • Use PCR to amplify multiple DNA samples
  • Use electrophoresis to visualize results
  • Complete in two 45 minute lab sessions
Curriculum Fit
Curriculum Fit
Conventional PCR Animation

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Complete Product Description
Crime Scene Investigator PCR Basics Gel

This introductory PCR kit allows students to simulate DNA profiling as commonly used in forensic labs. The lab is designed to introduce the concepts of PCR to students in two lesson periods without the need for complex genomic DNA extraction steps. The polymerase chain reaction (PCR) is widely used in forensics, diagnostics, and archeological procedures.

DNA profiling determines the exact genotype of a DNA sample, and distinguishes one human being from another by identifying a DNA "bar code" that is unique to every individual. This powerful tool assists in investigations of crime scenes, missing persons, mass disasters, immigration disputes, and paternity testing. Crime scenes often contain tiny biological specimens (such as blood, semen, hairs, saliva, bones, pieces of skin) from which DNA can be extracted. The reason DNA profiling is so useful for forensic scientists is that a definitive DNA profile can be obtained from a minute biological sample, using the power of DNA amplification afforded by PCR.

What kinds of human DNA sequences are used in crime scene investigations? There are ~3 billion bases in the human genetic blueprint, and more than 99.5% of them do not vary among human beings. Within the variant areas of the genome are the special polymorphic ("many forms") sequences used in forensic applications. The DNA sequences used for forensic typing are derived from regions of our chromosomes that do not control any known traits and have no known functions. They contain segments of short tandem repeats, called STRs. STRs are very short DNA sequences that are repeated in direct head-to-tail fashion.

The example below shows a locus (known as TH01) actually used in forensic DNA profiling. Its specific DNA sequence contains five repeats of [TCAT]

For the TH01 STR locus, there are many alternate forms (alleles) that differ from each other by the number of [TCAT] repeats present in the sequence. More than 20 different alleles of TH01 have been discovered in people worldwide. Each of us still has only two alleles, one inherited from our mother and one inherited from our father.

Two sample TH01 genotypes

How are STR alleles detected? Each STR allele has a different length depending on the number of tandem repeats it contains. When the alleles are amplified by PCR, alleles of different lengths can be distinguished by electrophoresis. The number of tandem repeats contained in each allele can be determined by comparing the locations of the DNA bands with a DNA size standard that corresponds to the known sizes of TH01 alleles.

The DNA samples contained in this kit are plasmids that have been engineered to mimic the natural variations in human DNA that exist between one human being and another at a single STR locus. In real crime scene applications, using the international CODIS system, DNA profiling is performed using 13 loci to increase the power of discrimination. Extension exercises in the kit curriculum provide access to real profiling data, enabling students to perform statistical analysis and apply the power of discrimination. With the addition of each locus to the analysis, the possibility that any two genotypes will match due to chance drops off significantly. This exercise allows students to grasp the concept of the power of discrimination — the more loci that are used, the greater the ability to discriminate between any two samples.

Large Class Preparation Guide

Learn tips and techniques for preparing agar plates and agarose gels in large quantities.


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PowerPoint Presentation for Workshops and for Teaching

Crime Scene Investigator PCR Basics Kit

This PowerPoint presentation is intended for educational use only. The presentation provides educators with background on the Crime Scene Investigator PCR Basics Kit and includes instructions for using the kit.

Crime Scene Investigator PCR Basics Kit Download PowerPoint presentation
Crime Scene Investigator PCR Basics Kit

The Crime Scene Investigator PCR Basics™ Kit is an introductory PCR kit that allows students to simulate DNA profiling as it is commonly used in forensic laboratories. The laboratory activity is designed to introduce the concepts of PCR, which is widely used in forensics, diagnostics, and archaeological procedures. The laboratory is performed without the need for complex genomic DNA extraction steps.

Features and Benefits

With this kit, students are able to:

  • Perform real-world DNA profiling
  • Use PCR to amplify multiple DNA samples
  • Use agarose gel electrophoresis to visualize results

Background Information

DNA profiling determines the exact genotype of a DNA sample, distinguishing one human being from another by identifying each person's unique DNA "bar code." This powerful tool aids investigations of crime scenes, missing persons, mass disasters, immigration disputes, and paternity. Crime scenes often contain tiny biological specimens (blood, semen, hairs, saliva, bones, pieces of skin) from which DNA can be extracted. The reason DNA profiling is so useful for forensic scientists is that a definitive DNA profile can be obtained from a minute biological sample using the power of DNA amplification afforded by polymerase chain reaction (PCR).

More Information

  • Two–session laboratory activity, 45 min per session
  • Provides sufficient materials for eight student workstations, up to four students per workstation
  • Includes curriculum with Teacher's Guide, Student Manual, and graphical Quick Guide
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation)  
Crime scene DNA sample, 250 µl 1
Suspect A DNA sample, 250 µl 1
Suspect B DNA sample, 250 µl 1
Suspect C DNA sample, 250 µl 1
Suspect D DNA sample, 250 µl 1
PCR master mix (Taq DNA polymerase dNTPs, buffer), 1.2 ml 1
Primers (blue), 25 µl 1
Allele ladder, 200 µl 1
Orange G loading dye, 1 ml 1
PCR tubes, 0.2 ml 50
Capless PCR tube adaptors, 1.5 ml 50
Colored micro test tubes, 2.0 ml 72
Foam micro test tube holders 8
Crime Scene Investigator PCR Basics Kit

166-2600EDU
Introductory PCR classroom study kit, includes crime scene and suspect samples, PCR master mix, primers, allele ladder, test tubes/holders, curriculum, and more, for 32 students; education use only

List Price:   $280.00
EDU Price:   $224.00   What is this?
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Crime Scene Investigator PCR Basics Kit Plus Small DNA Electrophoresis Reagent Pack

166-2650EDU
Classroom study kit includes Crime Scene Investigator PCR Basics kit (#166-2600) and agarose gel electrophoresis reagents for 48 (1%) or 16 (3%) 7 x 10 cm agarose gels; education use only

List Price:   $337.00
EDU Price:   $269.60   What is this?
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Number Description Options
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories, Rev A Click to download
5396 Fast Gel Protocol. Run Agarose DNA Gels in Under 20 Minutes, Biotechnology Explorer, Rev A Click to download
5292 Crime Scene Investigator PCR Basics Kit, Biotechnology Explorer, Quick Guide, Rev A Click to download
10002461 Instruction Manual, Crime Scene Investigator PCR Basics Kit, Biotechnology Explorer, Rev D Please login to download this document [ Add to Cart (Free) ]
5891 Refresh Kit Components — Large Class/Multiple Class Preparation Guide, Rev A Click to download
5872 Biotechnology Explorer Refresh Kit Components Purchasing Guide, Rev A Click to download
6602 UView™ 6x Loading Dye and Stain Guide, Rev A Click to download