Cloning and Sequencing Explorer Series

Print
Cloning and Sequencing Explorer Series - Cloning and Sequencing Explorer Series

Overview

Cloning and Sequencing Explorer Series Flowchart
Cloning and Sequencing Explorer Series Flowchart
Cloning and Sequencing Explorer Series Contents
Lab contains sufficient materials for 12 workstations (2–4 students per workstation).
Nucleic Acid Extraction Module 1
GAPDH PCR Module 1
Small UView™ DNA Electrophoresis Reagents Module 1
PCR Kleen™ Spin Purification Module 1
Ligation and Transformation Module 1
Microbial Culturing Module 1
Aurum™ Plasmid Mini Purification Module 1
Sequencing and Bioinformatics Module 1
1.5 ml EZ Micro™ test tubes, pkg. of 500 1
Required Accessories Not Included in Kit:
Plant samples
95–100% lab grade ethanol
Horizontal gel electrophoresis chambers
Thermal cycler
Adjustable micropipets
Pipet tips, aerosol barrier
Incubation oven
Water bath and dry bath
Microcentrifuges capable of greater than 12,000 x g
Power supplies
Gel documentation system
Shaking water bath or shaking incubator
Recommended (Optional) Accessories:
Vortexer
Microwave oven/autoclave
Gel documentation system
Spectrophotometer and cuvettes
Available Separately:
Nucleic Acid Extraction Module (1665005EDU) 1
GAPDH PCR Module (1665010EDU) 1
Small UView DNA Electrophoresis Reagents Module (1660462EDU) 1
PCR Kleen Spin Purification Module (7326300EDU) 1
Ligation and Transformation Module (1665015EDU) 1
Microbial Culturing Module (1665020EDU) 1
Aurum Plasmid Mini Purification Module (7326400EDU) 1
Sequencing and Bioinformatics Module (1665025EDU) 1
1.5 ml EZ Micro™ test tubes, pkg. of 500 (2239480EDU) 1
Cloning and Sequencing Explorer Planning Guide

Overview

The intention of this planning guide is to provide an overview of the entire student research project including details of timelines, materials included, and equipment requirements so that instructors may prepare their curriculum and estimate budgetary requirements in advance. These are estimates based on current information available, and may change.


To read more click here

Conventional PCR Animation

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player



The Cloning and Sequencing Explorer Series gives your students the tools to perform novel research and contribute to the world's body of scientific knowledge. Over 8 modules, students use classic and cutting-edge molecular biology techniques to clone and sequence a portion of the GAPC gene from a plant of their choice. GAPC is a housekeeping gene in the glyeraldehyde-3-phosphate dehydrogenase (GAPDH) family and is well conserved among plants.

The Cloning and Sequencing Explorer Series guides students through the following experimental workflow steps:

  1. Extract genomic DNA from plants
  2. Amplify a GAPDH gene using PCR and analyze using electrophoresis
  3. Purify the PCR product
  4. Ligate the PCR product into a plasmid
  5. Transform the ligated plasmid into bacteria
  6. Grow minipreps of the transformed bacteria
  7. Isolate plasmid DNA and analyze by restriction digestion
  8. Sequence plasmid DNA (sequencing service not included)
  9. Analyze GAPDH gene sequence using bioinformatics

Features and Benefits

Designed by undergraduate and community college educators, this course was specifically developed to engage your students in authentic research, while ensuring success for both you and them. Tailored experimental controls allow your students to:

  • Continue the experiment to its conclusion even with negative results
  • Make mistakes, learn from them, and still complete every step of the workflow
  • Experience firsthand the satisfaction of completing a successful research project
  • Apply molecular biology skills and techniques to complete a real-world research project

The Cloning and Sequencing Explorer Series allows students to apply molecular biology skills and techniques to complete a real-world research project, potentially encouraging them to pursue a career in research.

Now with Geneious!

geneiousThe Cloning and Sequencing Explorer Series now includes a 4-month subscription to Geneious Bioinformatics Software to support your students' scientific discovery.

For more information, visit the Sequencing and Bioinformatics Module product page.

More Information

  • 6–8 week series of laboratory activities made up of eight modular kits
  • Provides sufficient materials for 12 student workstations, up to four students per workstation
  • Sequencing service not included.

Additional Resources

x

Account price failed to display. Please try reloading the page. Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at   or send an
email to 

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Cloning and Sequencing Explorer Series

1665000EDU
Molecular biology study kit, includes 8 modules with lab activities from DNA extraction and cloning to computer-based sequence analysis, curriculum (sequencing service not included); education use only

List Price:
 
$2,383.00
LoadingLoading...
Your Price:
 

Communication error

Reload Page

EDU Price:   $1,906.40   What is this?
Quantity:   Add to Cart
Add to Hot List   Add to Quote
Hazardous  
Number Description Options
10000069187 Bio-Rad Explorer™ Cloning and Sequencing Explorer Series, Rev F Click to download
5773 Cloning and Sequencing Explorer Series Planning Guide, Bio-Rad Explorer™ , Ver C Click to download
6672 Map of AP-Aligned Bio-Rad Kits with Essential Knowledge, Rev A Click to download
6673 Map of AP-Aligned Bio-Rad Kits with Investigative Laboratories, Rev A Click to download
6602 UView™ 6x Loading Dye and Stain Guide, Bio-Rad Explorer™, Rev A Click to download