Clarity™ Western ECL Substrate

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Clarity Western ECL Substrate

Overview



Specifications
  Clarity™ Western ECL Substrate
Lower detection limit Mid femtogram
Signal duration 24 hr
Primary detection method Digital imager and film
Suggested antibody dilution Primary: 1/1,000–1/50,000; Secondary: 1/50,000–1/250,000
Recommended membrane Nitrocellulose, PVDF, or LF PVDF
Chemiluminescence Chemistry

Chemiluminescence detection. The secondary antibody, which binds the antibody specific for the protein of interest, is conjugated to an enzyme; when it acts on the substrate, light is emitted.

Chemiluminescent western blot detection is a highly sensitive alternative to isotopic detection of proteins bound to blotting membranes. The Bio-Rad Clarity western ECL substrate is compatible with any HRP-conjugate secondary antibody and ideal for both digital and film-based imaging. Substrate components are stable for at least one year at room temperature.

The Clarity substrate provides excellent sensitivity, very low background levels, and an extremely long signal duration. This produces exceptionally clear images and allows re-imaging without loss of signal. The combination of a bright, long signal and low background makes the Clarity substrate the perfect choice for most blotting applications.

Features of the Clarity western ECL substrate:

  • Femtogram level sensitivity
  • 24 hour signal duration
  • 1 year shelf life at room temperature
  • Ideal for digital and film-based imaging

 

170-5060 image
Clarity Western ECL Substrate, 200 ml

170-5060
Contains 100 ml Clarity western peroxide reagent and 100 ml Clarity western luminol/enhancer reagent

List Price:   $154.00
Quantity:   Add to cart
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170-5061 image
Clarity Western ECL Substrate, 500 ml

170-5061
Contains 250 ml Clarity western peroxide reagent and 250 ml Clarity western luminol/enhancer reagent

List Price:   $294.00
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Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate

170-5047
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

List Price:   $233.00
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Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate

170-5046
2 ml, horseradish peroxidase secondary antibody conjugate, for use with Immun-Star HRP chemiluminescent detection kits

List Price:   $233.00
Quantity:   Add to cart
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Number Description Options
6305 Clarity Western ECL Substrate Brochure, Rev A Click to download
10026691 Clarity Western ECL Substrate Transition Guide, Rev A Click to download
10026527 Clarity Western ECL Substrate Quick Start Guide, Rev A Click to download
10026389 Clarity Western ECL Substrate Instruction Manual, Rev A Click to download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download [ Add to Cart (Free) ]
6148 Transfer of High Molecular Weight Proteins to Membranes: A Comparison of Transfer Efficiency Between Blotting Systems, Rev B Click to download [ Add to Cart (Free) ]
10000090 Instruction Manual, Immun-Star Goat Anti-Rabbit (GAR)-HRP Conjugate, Rev A Click to download
10000091 Instruction Manual, Immun-Star Goat Anti-Mouse (GAM)-HRP Conjugate, Rev A Click to download