ChemiDoc™ XRS+ System

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Overview

Protein Standards for Blotting

Chemiluminescent Detection

The secondary antibody, which binds the antibody specific for the protein of interest, is conjugated to an enzyme; when it acts on the substrate, light is emitted.

Imaging System Selection Guide
  ChemiDoc™ MP GelDoc™ EZ GS-900 Gel Doc XR+ and ChemicDoc XRS+ PharosFX™, PharosFX Plus, Personal Molecular Imager
ChemiDoc™
MP
GelDoc™
EZ
GS-900™ Gel Doc
XR+
ChemiDoc
XRS+
PharosFX™ PharosFX Plus Personal Molecular Imager™
Nucleic Acid Detection
Ethidium bromide 5 4 - 4 4 5 5 -
SYBR® Green I stain 5 4 - 4 4 5 5 -
SYBR® Safe stain 5 4 - 4 4 5 5 -
Coumarin stain 5 4 5 - - - - -
Fast Blast™ DNA stain 4 4 5 4 4 - - -
Protein Staining, 1-D Gels
Stain-free gels 5 5 - 5 5 - - -
Coomassie Blue stain 4 4 5 4 4 2 2 -
Silver stain 4 4 5 4 4 2 2 -
SYPRO Ruby protein blot stain 4 4 - 4 4 5 5 -
Flamingo™ fluorescent gel stain 4 3 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 4 - 5 5 - - -
Protein Staining, 2-D Gels
Coomassie Blue stain 4 3 5 3 3 2 2 -
Silver stain 4 3 5 3 3 2 2 -
SYPRO Ruby protein blot stain 4 3 - 3 3 5 5 -
Flamingo™ fluorescent gel stain 4 2 - 3 3 5 5 -
Oriole™ fluorescent gel stain 5 3 3 3 3 3 3 3
Pro-Q stain 4 2 - 2 3 5 5 -
Cy2, Cy3, Cy5 label 4 - - - - 5 5 -
Blot Detection
Stain-free blots 4 5 - 4 5 - - -
Colorimetric blots 3 3 5 3 3 2 2 2
Coomassie Blue stain 4 - 5 4 4 2 2 -
Silver stain 5 - 5 4 4 2 2 -
SYPRO Ruby protein blot stain* 5 - - - - 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Chemifluorescence* 5 - - 1 1 4 4 -
Qdot* 5 2 - 2 2 5 5 -
Multiplex fluorescence 5 - - - - - - -
Micro- and Macroarray** Detection
Radiolabel - - - - - - 5 5
Fluorescence 5 - - 2 2 5 5 -
Immun-Star™ chemiluminescence 5 - - - 4 - - -
Colony Counting
Colorimetric detection 5 - - 4 4 3 3 -
Fluorescent detection 5 - - 4 4 5 5 -
Isotopic
Radiolabel - - - - - - 5 5
X-ray film 4 - 5 4 4 3 3 -

- Not recommended; 1–5, recommendation level (5 = highest).
*    Optimal with low fluorescence PVDF membrane.
**   With spot features ≥400 µm.

Coomassie Blue-Stained Gel Image

Coomassie Blue-stained gel imaged using the VersaDoc™ MP system.

The ChemiDoc XRS+ system is based on CCD high-resolution, high-sensitivity detection technology and modular options to accommodate a wide range of samples and support multiple detection methods including fluorescence, colorimetry, densitometry, chemiluminescence, and chemifluorescence. The system is controlled by Image Lab™ software to optimize imager performance for fast, integrated, and automated image capture and analysis of various samples.

The system accommodates a wide array of samples, from large handcast polyacrylamide gels to small ReadyAgarose™ gels and various blots. The system is an ideal accompaniment to PCR, purification, and electrophoresis systems, enabling image analysis and documentation of restriction digests, amplified nucleic acids, genetic fingerprinting, RFLPs, and protein purification and characterization.

Key benefits include:

  • Gel and blot imaging and analysis are quick and accurate
  • Automated, hands-off routines; no training is required
  • Save and recall all the steps in the workflow for repeatable and reproducible results
  • Optimize the system at setup for image data that is always accurate, reproducible, and free of imaging artifacts
  • Wide range of applications with special accessories to preserve sample integrity for downstream research while ensuring user safety
  • Comprehensive, automated quantitative analysis of protein and DNA samples in seconds
  • Customize and organize data in reports
  • Obtain publication-quality results quickly

The ChemiDoc XRS+ system optimizes reproducibility and reliability of experimental data, enabling quantitative comparisons between different experiments. Using proprietary algorithms, this imaging system is calibrated at setup to ensure:

  • Images are in focus at all times, regardless of zoom level or sample position
  • Appropriate flat fielding correction is automatically and consistently applied to image data for every application
  • Imaging artifacts are automatically corrected

The ChemiDoc XRS+ system offers fast and sensitive chemiluminescence detection in addition to gel and blot documentation of fluorescent and colorimetric samples. The system includes a supersensitive 16-bit CCD camera that is supercooled for detection of faint samples. The ChemiDoc XRS+ system can be used for imaging with a wide variety of applications that require high resolution and sensitivity (such as chemiluminescent western blots).

The ChemiDoc XRS+ system eliminates the need to use costly and unreliable X-ray film technologies while providing quantitative and reproducable data in seconds. The system features a signal accumulation mode (SAM), which guides a user through determining optimum exposure time and capturing a desired image of a chemiluminescent sample.

Applications  
Chemiluminescence Yes
Fluorescence* Yes
Colorimetry/densitometry Yes
Gel documentation Yes
Hardware Specifications  
Maximum sample size up to 28 x 36 cm
Maximum image area up to 26 x 35 cm
Maximum image area (For standard UV-excited gels) 25 x 26 cm
Excitation source Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen and XcitaBlue™ UV/blue conversion screen available
Illumination control 5 modes (trans-UV, epi-white, and no illumination for chemiluminescence are standard); trans white and XcitaBlue conversion screens are optional
Detector Supercooled CCD
Pixel size (H x V) 6.45 x 6.45 µm
Cooling system Peltier
Camera cooling temperature -30°C controlled
Filter selector 3 positions (2 for filters, 1 without filter, for chemiluminescence)
Emission filters 1 included (standard), 3 optional
Dynamic range >4.0 orders of magnitude
Pixel density (gray levels) 65,535
Dynamic flat fielding Application specific, for all applications
Instrument size (L x W x H) 36 x 60 x 96 cm
Instrument weight 32 kg
Operating Ranges  
Operating voltage 110/115/230 V AC nominal
Operating temperature 10–28°C (21°C recommended)
Operating humidity <70% noncondensing
Automation Capabilities  
Workflow automated selection Application driven, user selected or recalled by a protocol
Workflow automated execution Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
Workflow reproducibility 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
Autofocus (patent pending) Precalibrated focus for any zoom setting or sample height
Image flat fielding** Dynamic; precalibrated and optimized per application
Autoexposure 2 user-defined modes (intense or faint bands)
*Use the optional XcitaBlue kit if performing SYBR® Safe DNA applications, because the UV to blue conversion screen allows you to visualize DNA samples while protecting against UV damage.

**U. S. patent 5,951,838.
 
Molecular Imager® ChemiDoc™ XRS+ System with Image Lab™ Software #170-8265
ChemiDoc™ XRS+ System with Image Lab™ Software

170-8265
Gel imaging system, PC or Mac, includes darkroom, UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab software

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