CHEF-DR II System

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Overview

Introduction to Pulsed Field Gel Electrophoresis

This is from Product Overlay
PFGE Standards Selection Guide
5 kb Ladder 8–48 kb Ladder Lambda Ladder S. cerevisiae
Chromosomal DNA
H. wingei
Chromosomal DNA
S. pombe
Chromosomal DNA
Comparison of Pulsed Field Standards
 
  Range Contents Amount Number of Applications
 
5 kb ladder 4.9–120 kb Concatemers of pBR328 20 µg in 200 µl 20–25
8–48 kb ladder 8.3–48.5 kb Mixed digest of phage λ 25 µg in 125 µl 125
Lambda (λ) ladder 0.05–1 Mb Concatemers of phage λcl857Sam7 5 agarose blocks 25–40
S. cerevisiae 0.225–2.2 Mb Saccharomyces cerevisiae chromosomal DNA 5 agarose blocks 25–40
H. wingei 1–3.1 Mb Hansenula wingei chromosomal DNA 5 agarose blocks 25–40
S. pombe 3.5–5.7 Mb Schizosaccharomyces pombe chromosomal DNA 5 agarose blocks 25–40
 

 

Certified Agarose Selection Guide
 
Packaging
25 g 161-3100 161-3103
161-3106   161-3111 161-3113 162-0137
100 g             162-0138
125 g 161-3101 161-3104 161-3107   161-3112 161-3114  
500 g 161-3102 161-3105       161-3115  
Analytical Separation
≥1,000 bp          
≤1,000 bp          
20–800 bp            
1 kb–2 Mb          
1 kb–5 Mb            
Preparative Separation
Quantum Prep methods      
Low-melt methods          
Agarose methods          
In-gel applications          
Other Applications
Postpreparative enzymatic treatments    
Tissue/cell culture            
Pulsed field sample preparation          
Blotting      



CHEF technology yields straight lanes over a wide range of DNA molecule sizes. The CHEF-DR® II system has been streamlined by condensing the functions of Pulsewave 760 technology and the power supply into the CHEF-DR II control module.

The CHEF-DR II system effectively resolves DNA fragments in the range of 5 kb to 6 Mb. This system lets you enhance resolution by entering two blocks of running conditions to be executed successively. The CHEF-DR II system exclusively employs the most common angle of electrophoresis for PFGE, 120°. This system is simple to program, and has proven to be a solid performer. Each CHEF-DR II system is supplied with an electrophoresis cell, drive module, control module, variable-speed pump, and accessory kit. The CHEF-DR II chiller systems also come with a cooling module.

Automation
The CHEF-DR II system, the most cost-effective PFGE instrument, requires direct user input of optimal run time, voltage gradient, and switch intervals for each run.

Customization
Users can program run conditions into the CHEF-DR II system. The product manual features extensive examples of run conditions that can be input based on a variety of size separation ranges.

Application Versatility
The CHEF-DR II system is optimized for the most popular range of separations with a fixed angle of 120°. This unit can be used for separations of fragments up to 6 Mb by adjusting the run conditions for low voltage and extended run times.

Drive Module  
Dimensions (W x D x H) 43 x 26 x 14 cm
Construction Aluminum chassis
Weight 7.8 kg (17.2 lb)
Maximum voltage 250 V DC
Maximum current 500 mA
Electrode potentials Dynamically regulated (feedback adjustment) +/- 0.5%
Input voltage range 90–132 VAC/47–63 Hz/0.7 A (maximum)
198–264 VAC/47–63 Hz/0.35 A (maximum)
Fuses 0.5 A Fast Blow for high voltage output (front panel)
1.0 A Slow-Blow (100/120 V) or
0.5 A Slow-Blow (220/240 V)
Environmental  
Operating temperature 50°F (10°C) to 90°F (32°C); 30–80% humidity
Storage temperature 32°F (0°C) to 140°F (60°C); 10–90% humidity
Control Module  
Dimensions (W x D x H) 31 x 13.5 x 17 cm
Maximum current 500 mA
Allowable voltage gradients 0.6–6 V/cm, in 0.1 V/cm increments
Data entry Keyboard
Power supply 250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable
Functional  
Switching range 0.1–65,000 sec (all electronic switching)
Maximum program blocks 2, with automatic execution
Maximum run time 999 hr per block
Input voltage range 90–132 VAC/47–63 Hz/1.7 A (maximum)
198–264 VAC/47–63 Hz/0.85 A (maximum)
Fuses   2.0 A Slow-Blow (100/120 V) or
1.0 A Slow-Blow (220/240 V)   
Operating   50°F (10°C) to 90°F (32°C); 30–80% humidity  
Storage   32°F (0°C) to 140°F (60°C); 10–90% humidity  
Electrophoresis Cell  
Dimensions (W x D x H) 44 x 43 x 11 cm, horizontal format
Construction Acrylic
Lid Safety interlocked
Weight 8.5 kg (18.7 lb)
Electrodes 24, platinum (0.01" diameter)
Temperature monitoring Via precision temperature probe mounted through lid (optional)
Accessories Included  
Variable speed pump 120 V, ground isolated; flow rate 0.75 liter/min, typical
Casting stand 14 x 13 cm
Comb 10 well comb and comb holder
Tygon tubing 365 cm
Sample plug mold 10 slot
Yeast DNA Standard S. cerevisiae YNN295, 2 plugs
Chromosomal Grade Agarose 5 g
Pulsed Field Certified Agarose 5 g
Leveling bubble 1
Fuses 0.5 A Fast Blow, 2
Manual 1
Cooling Module (optional)  
Chassis Aluminum
Dimensions (W x L x H) 23 x 42 x 24 cm
Weight 14 kg (30.1 lb)
Compressor 1/12 HP
Refrigerant CFC 12
Heat exchanger Stainless steel
Operating range  5°C–25°C 
Cooling rate (pre-cool)   0.75°C/min from ambient to 14°C 
Cooling capacity (input power)  75 W at 14°C 
Input voltage range  90–132 VAC/47–63 Hz/4 A
198–264 VAC/47–63 Hz/2 A 
Fuses  6.3 A Slow-Blow (100/120 V) or
3.0 A Slow-Blow (220/240 V) 
CHEF-DR II Chiller System

170-3725
120 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

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 CHEF-DR II System
CHEF-DR II System

170-3615
220/240 V, complete PFGE system, includes pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

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 CHEF-DR II Chiller System
CHEF-DR II Chiller System

170-3727
220/240 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

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 CHEF-DR II Chiller System
CHEF-DR II Chiller System

170-3728
100 V, complete PFGE system with cooling module, pump, gel casting accessories, 2 plugs S. cerevisiae DNA size standard, 5 g pulsed field Certified agarose, 5 g pulsed field Certified megabase agarose

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Number Description Options
M1703729 Instruction Manual, CHEF-DR II Pulsed Field Electrophoresis Systems, Rev B Click to download
5901 Pulsed Field Gel Electrophoresis Brochure, Rev B Click to download [ Add to Cart (Free) ]
4000066 Instruction Manual, Cooling Module, Rev C Click to download
1736 PFGE Analysis of Chromosomal DNA From a Single Hair Root Click to download
1647 Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae Click to download
1648 Application of Two-Dimensional Pulsed Field Electrophoresis for Determining Molecular Karyotypes Bulletin, Rev A Click to download
1769 Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping Click to download
1589 Preparation and Separation of Mammalian DNA by Pulsed Field Gel Electrophoresis Bulletin, Rev A Click to download
9204213 Instruction Manual, CHEF Variable Speed Pump, Rev B Click to download
LIT526 Instruction Manual, Standard Casting Stand Combination Comb Holder Wide/Long Combination Casting Stand Click to download
1624 Model 1000 Mini Chiller Product Information Sheet Click to download