CheckN'Safe™ Kits for Water Quality Testing

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CheckN'Safe™ Kits

Overview

Key Benefits
  • Easy to handle approach, skills in microbiology are not required
  • Membrane filtration only, no need for investment in additional equipment
  • Compact and robust, no specific water lab required
  • Fast results:
    • 2,000 E. coli /100ml detected in 4 hr 20 min
    • 500 E. coli /100ml detected within 5 hr
    • 250 Enterococci / 100ml detected within 9 hr
Protocol

 

 

Validation

XplOrer64 method has been certified by AFNOR as an alternative method to the following reference standards, according to the reference for validation “Protocol for the Validation of a commercial method versus a reference method in the water microbiology field (revision 0)” (adopted by AFNOR Certification on 28/07/2008) :

  • EN ISO 9308-3, for the detection and enumeration of Escherichia coli at 44°C (with CheckN’Safe E.coli reagent) in bathing waters, under certification No: BRD 07/18 - 11/09. Valid until: 06/11/2013
  • EN ISO 7899-1, for the detection and enumeration of intestinal Enterococci at 37°C (with CheckN’Safe Enterococci reagent) in bathing waters, under certification No: BRD 07/19 - 11/09. Valid until: 06/11/2013

 

 



CheckN'Safe Kits, used with the XplOrer64™ System, are designed for real-time continuous detection and quantification of E. coli, intestinal Enterococci and coliforms in marine or fresh recreational waters. Up to 64 samples can be processed simultaneously. Detection and enumeration of E. coli and/or Enterococci is simple to perform, providing rapid real-time quantitative results. The system is based on repeated measurement of impedance in each CheckN'Safe tube.

Intended Use

CheckN'Safe Kits and the automated XplOrer64 System detect and quantify two indicators of fecal contamination E. coli and intestinal Enterococci in recreational bathing water in real time. These kits provide rapid water quality monitoring, and for same-day decisions about opening or closing recreational areas.

Key Benefits

  • Each kit contains very selective media to enable growth of targeted environmental strains
  • The first alternative method to MUG/EC and MUD/SF microplates (EN ISO 9308-3 and 7899-1) to be certified by AFNOR validation for monitoring of bathing water
  • Easy to use method to quantify E. coli and/or intestinal Enterococci without confirmation
  • Random access loading of up to 64 CheckN'Safe Tubes for high flexibility and throughput
  • Automated method from testing to final report; information available in real-time (every 10 min)
  • The higher the bacterial concentration, the sooner the time to determination of the numbers of E. coli and/or Enterococci in water samples

Principle

CheckN'Safe Kits are formulated for selective growth of E. coli, intestinal Enterococci, or coliforms. The kits are used with the XplOrer64 System for continuous detection of fecal contaminants in water samples. The XplOrer64 method is an automated system that uses impedance measurements in a liquid medium to enumerate bacteria over time. Up to 100 ml of water sample is filtered, and then the filter is placed in a tube of CheckN’Safe Culture Medium that is specific for the bacteria to be detected. One ml samples can be directly inoculated. Impedance is measured every 10 minutes giving continuous real-time data. The system has two incubators so E. coli and intestinal Enterococci can be assayed at the same time. Impedance is measured every 10 minutes giving continuous real-time data. The user-friendly XplOrer64 Software interprets the results, according to the water quality criteria classification set by regulations (CEE/76/160 Directive or 2006/7/CE, or AFSSET 2007 recommendations).

The use of CheckN’Safe Kits with the XplOrer64 System has several advantages over other water quality testing methods including;

  • Impedance can be used for analysis of samples that are turbid or opaque and/or contain small particulates. These types of samples can interfere with methods that use detection of light and with low counts on plates
  • Results can be obtained much more quickly than traditional manual culture methods permitting faster response times to contamination. Same-day results for active management of recreational water
  • This automated method is less labor intensive, requiring less skill and training of personnel
  • Database creation (XplOrer64 Smart View) for easily accessible reliable traceability

Product Options

  • CheckN'Safe E.coli Kit, 60 tests (3554720)
  • CheckN'Safe Enterococci Kit, 60 tests (3554721)
  • CheckN'Safe Coliforms Kit, 60 tests (3554722)

Related Products

  • CheckN'Safe Screw Caps (3593457)
  • XplOrer64 System Full Configuration 230V (3593450)
  • XplOrer64 System Configuration 230V (w/o PC) (3594452)

Related Categories

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

(Discontinued) CheckN'Safe™ E.coli Kit

3554720
Pkg of 1, kit for use with the XplOrer64™ System, 60 x 10 ml vials of E. coli selective medium

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) CheckN'Safe™ Enterococci Kit, 60 tests

3554721
Pkg of 1, kit for use with the XplOrer64™ System, 60 x 9 ml vials of intestinal Enterococci selective medium

This product is no longer available. Please contact technical support for more information on replacement product.
(Discontinued) CheckN'Safe™ Screw Caps

3593457
Pkg of 40, sterile screw caps for CheckN'Safe Kit test tubes

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
FSD_PF108 CheckNSafe™ E.coli Kit Quick Guide, Rev B Click to download
FSD_Article_XplO_2010 FSD_Art_XplOrer64_2010 Click to download
FSD_16195 MUG/MUD Kits for E. coli or Enterococci Quick Guide Click to download
FSD_17932 Water Safety Booklet - Guide for Products in Water Testing Click to download