CFX384 Touch™ Real-Time PCR Detection System

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CFX384 Touch™ Real-Time PCR Detection System

The CFX384 Touch Real-Time PCR Detection System combines the C1000 Touch™ Thermal Cycler with the CFX384™ Optical Reaction Module to bring ease of use and rapid data acquisition to researchers performing high-throughput real-time PCR in a 384-well format. The CFX384 Touch System builds on the precise thermal control of the C1000 Touch Thermal Cycler to deliver the most sensitive, reliable detection for real-time PCR applications. Quickly set up runs and monitor amplification traces in real time on the integrated LCD touch screen. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful yet easy-to-use software, the CFX384 Touch System was designed to advance your qPCR experiments.

Key Features and Benefits

With the CFX384 Touch Real-Time PCR Detection System you can:

  • Obtain precise quantification and multiplex target discrimination – optical system uses long-lasting solid-state technology with 5 filtered LEDs and 5 filtered photodiodes
  • Configure the system to fit your laboratory needs – optimized design lets you run without a computer, run up to 4 systems from 1 computer, or integrate with the CFX Automation System II for higher throughput
  • Confirm system performance and data reliability – internal system qualification and system test software, automated data quality control, and various validation tool services are available
  • Obtain accurate results with volumes as low as 3 µl – minimize sample and reagent usage
  • Easily integrate with your laboratory information management system (LIMS) – integrate with any LIMS using built-in LIMS file management
  • Combine the CFX384 Touch System with good laboratory practice standards – use CFX Manager™ Software, Security Edition, which complies with U.S. FDA 21 CFR Part 11 regulations, for data collection and analysis

Flexible CFX Systems Configurations Meet Your Throughput Needs

The CFX Automation System II works with all CFX Real-Time PCR Detection Systems to enable walk-away, high-throughput qPCR operation. Integrate this robotic plate handler with one or two CFX Systems to maximize throughput while maintaining a compact footprint.

Flexible Configurations Meet Your Throughput Needs

CFX Manager Software Works Seamlessly with PrimePCR™ Assays and Panels

The expert design and wet-lab validation of all PrimePCR Assays ensure optimal assay performance. The integration of CFX Manager Software with PrimePCR Assays aids in the analysis and interpretation of experimental results.

  • Begin analyzing data in a single step by importing target, reference and control information from a PrimePCR run file directly into CFX Manager Software
  • Combine data from multiple plates into a Gene Study to rapidly screen large numbers of targets or samples
  • Use powerful data visualization tools, such as hierarchical clustering and color-matched expression levels, to identify individual targets or clusters to consider for further investigation

Service Plans and Validation Tools for CFX Systems, Thermal Cyclers, and Real-Time PCR Instruments

Thermal Cycler  
Chassis C1000 Touch™
Maximum ramp rate, °C/sec 2.5
Average ramp rate, °C/sec 2
Heating and cooling method Peltier
Lid, °C Heats up to 105
Temperature  
Range, °C 0–100
Accuracy, °C ±0.2 of programmed target at 90°C
Uniformity, °C ±0.4 well-to-well within 10 sec of arrival at 90°C
Gradient  
Operational range, °C 30–100
Programmable span, °C 1–24
Optical Detection  
Excitation 5 filtered LEDs
Detection 5 filtered photodiodes
Range of excitation/emission wavelengths, nm 450–690
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range  10 orders of magnitude
Scan Time  
All channels, sec <20
FAM/SYBR® Green only, sec 8
Software  
Operating systems Windows 7, Windows 8
Multiplex analysis Up to 4 targets per well
System  
Licensed for real-time PCR Yes
Sample capacity, wells 384
Sample size, µl 1–30 (5–20 recommended)
Communication interface USB 2.0
Electrical approvals IEC, CE
Dimensions (W x D x H), cm/in 33 x 46 x 36/13 x 18 x 14
Weight, kg/lb 21/47
CFX384 Touch™ Real-Time PCR Detection System

185-5485
Modular thermal cycler platform, includes C1000 Touch™ Thermal Cycler chassis, CFX384™ Optical Reaction Module, CFX Manager™ Software, qbase+ Software license, cables

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CFX384 Touch™ Real-Time PCR Detection System

185-5484
Modular thermal cycler platform, includes C1000 Touch™ Thermal Cycler Chassis, CFX384™ Optical Reaction Module, CFX Manager™ Software, qbase+ Software license, cables, reagents, consumables

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CFX384™ Optical Reaction Module

184-5384
384-well optical module for real-time PCR, includes CFX Manager™ Software, qbase+ Software license, cables, reagents, consumables, for use with C1000 Touch™ Thermal Cycler chassis

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CFX384™ Optical Reaction Module

184-5385
384-well optical module for real-time PCR, includes CFX Manager™ Software, qbase+ software license, cables, for use with C1000 Touch™ Thermal Cycler chassis

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Number Description Options
6096 CFX384 Touch Real-Time PCR Detection System Brochure, Rev C Click to download [ Add to Cart (Free) ]
6072 CFX384™ Touch Real-Time PCR Detection System Specifications Sheet, Rev C Click to download [ Add to Cart (Free) ]
6077 CFX384 Touch Real-Time PCR Detection System Flier, Rev A Click to download
10027342 CFX Qualification Plate — 384-Well Plate Format Product Insert, Rev B Click to download
10022527 Amplification Plastics and Reagents Selection Guide, Rev B Click to download
6094 1000-Series Thermal Cyclers Brochure, Rev B Click to download [ Add to Cart (Free) ]
5690 CFX Manager Software, Security Edition Product Information Sheet, Rev A Click to download
10021337 Instruction Manual, CFX96 Touch, CFX96 Touch Deep Well, CFX Connect, and CFX384 Touch Real-Time PCR Detection Systems, Rev E Click to download
10021419 CFX384 Touch Real-Time PCR System Installation Quick Guide, Rev C Click to download
10014290 CFX Manager Software Protocol for the CFX384 System, Quick Guide, Rev C Click to download
10014293 CFX Manager Software Plate for the CFX384 System, Quick Guide, Rev C Click to download
10014294 CFX Manager Software Data Analysis for the CFX384 System, Quick Guide, Rev C Click to download
10014295 CFX Manager Software Gene Expression Analysis for the CFX384 System, Quick Guide, Rev D Click to download
10022138 Checking Firmware and Software Updates for the C1000 Touch Thermal Cycler and the CFX96 Touch, CFX96 Touch Deep Well, and CFX384 Touch Real-Time PCR Detection Systems, Quick Guide, Rev C Click to download
5962 Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient, Rev A Click to download
6047 Optical Design of CFX96 Real-Time PCR Detection System Eliminates the Requirement of a Passive Reference Dye, Rev A Click to download [ Add to Cart (Free) ]
6009 High Resolution Melt Parameter Considerations for Optimal Data Resolution, Rev A Click to download [ Add to Cart (Free) ]
6004 A Practical Guide to High Resolution Melt Analysis Genotyping, Rev A Click to download [ Add to Cart (Free) ]
6144 Plate Preparation for the CFX384 Real-Time PCR Detection System Using a Tecan Robotics System, Rev A Click to download [ Add to Cart (Free) ]
6149 Plate Preparation for the CFX384 Real-Time PCR Detection System Using the IDEX Health & Science Innovadyne Dispenser, Rev A Click to download
5859 A Practical Approach to RT-qPCR — Publishing Data That Conform to the MIQE Guidelines, Rev D Click to download [ Add to Cart (Free) ]
5692 Real-Time qPCR as a Tool for Evaluating RNAi-Mediated Gene Silencing, Rev A Click to download
5279 Real-Time PCR Applications Guide, Rev B Click to download [ Add to Cart (Free) ]
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download [ Add to Cart (Free) ]