CFT Ceramic Fluoroapatite Support

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Overview

Flow rate on IgG Capture
Upper panel, binding capacity of CFT Type I (orange) and Type II (blue). Lower panel, effect of flow rate on IgG capture using CFT Type II. Bovine IgG (1 mg/ml) was run on an 11 x 47.6 mm column packed with 4 g CFT until an A280 of 0.80 was reached. The loading buffer was 10 mM sodium phosphate, pH 6.75. Elution was performed using a step gradient from 10 to 400 mM sodium phosphate. The binding capacities were 37.0, 31.2, and 31.0 mg/ml, respectively, at 150, 300, and 450 cm/hr. Chromatography was performed on the BioLogic DuoFlow system.



CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent.

CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5, with minimal effect on the solubility or lifetime of the support.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features of CFT include:

  • Acidic protein separation for applications requiring pH as low as 5.0
  • High-density particles for fast, simple column packing
  • Sintering at 700°C (Type II) for heavy-duty, durable supports
  • Rigid particles for fast cleaning and equilibration
  • Inorganic calcium phosphate for distinct selectivities

Regulatory support files are available upon request. Bio-Rad Laboratories is an ISO9001 registered corporation.

Specifications and Characteristics  
Functional groups Ca2+, PO43-, F-
Nominal mean particle size 40 ± 4 µm
Nominal pore diameter range 600–800 Â
Recommended linear flow rate range 50–300 cm/hr
Operating pH range 5–14
Chemical compatibility (>24 hr) 2 N NaOH, 8 M urea, 6 M guanidine HCl
Regeneration 400 mM sodium phosphate, pH 6.8
Sanitation 1–2 N NaOH or KOH
Autoclavibility (121°C, 20 min) Yes
Packing density (g/ml packed bed) 0.86 g/ml
Dynamic binding capacity 14–21.5 mg lysozyme/g
Maximum operating pressure 55 bar (800 psi)
CFT Ceramic Fluoroapatite #158-5200
CFT Ceramic Fluoroapatite

158-5200
10 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

List Price:   $702.00
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CFT Ceramic Fluoroapatite

157-5000
100 g, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

List Price:   $2,001.00
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CFT Ceramic Fluoroapatite

157-5100
1 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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CFT Ceramic Fluoroapatite

157-5500
5 kg, fluoroapatite and hydroxyapatite composite, CFT ceramic fluoroapatite multimodal chromatography media, Type II, 40 μm particle size

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Number Description Options
3111 CFT Ceramic Fluoroapatite Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
4110183 Instruction Manual, CFT Ceramic Fluoroapatite, Rev A Click to download
5853 Chimeric IgG Monoclonal Antibody Purification: A Comparative Study Using CHT Ceramic Hydroxyapatite and CFT Ceramic Fluoroapatite Chromatographic Media, Rev A Click to download [ Add to Cart (Free) ]