Blot Absorbent Filter Paper

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Overview

Blotting Membrane and Filter Paper Selection Guide
Membrane Binding Capacity
(µg/cm2)
Applications
Nitrocellulose 80–100 Immunoblotting
Supported nitrocellulose 80–100 Immunoblotting
Immun-Blot® PVDF 150–160 Immunoblotting
Immun-Blot® low fluorescence PVDF 150–160 Fluorescent immunoblotting
Sequi-Blot™ PVDF 170–200 Protein sequencing
Zeta-Probe® 150 DNA/RNA hybridization
Zeta-Probe GT 150 DNA/RNA hybridization
C/P Lift 150 Colony/plaque screening

Blotting Apparatus Precut Membrane Sizes Precut Filter Paper Sizes Membrane/Filter Paper Sandwiches
Mini Trans-Blot® cell 7 x 8.4 cm 7.5 x 10.5 cm 7 x 8.5 cm
Criterion™ blotter 8.5 x 13.5 cm 9.5 x 15.2 cm 8.5 x 13.5 cm
Trans-Blot® cell 13.5 x 16.5 cm 15 x 20 cm  
Trans-Blot® Plus cell 25 x 28 cm 26.5 x 28  
  26.5 x 28 cm 26.5 x 28 cm  
Trans-Blot® SD cell 7 x 8.5 cm 14 x 16 cm 7 x 8.5 cm
  11.5 x 16 cm 7 x 8.4 cm 8.5 x 13.5 cm
  15 x 15 cm 8 x 13.5 cm  
  15 x 9.2 cm 18 x 18.5 cm  
  20 x 20 cm    
Mini-PROTEAN® II
multiscreen apparatus
7 x 8.4 cm 7 x 8.4 cm 7 x 8.5 cm
  7 x 8.5 cm    
Bio-Dot® apparatus 9 x 12 cm    
Bio-Dot® SF apparatus 9 x 12 cm 7.7 x 11.3 cm  

For more information, download bulletins 1939 and 2212.

Bio-Rad blot absorbent filter paper is high-quality 100% cotton fiber for use in electrophoresis and blotting applications. These filter papers are produced using ultrapure water and contain no additives that can interfere with any application. The uniform, smooth, white sheets are suitable for use with alcohol and other organic solvents involved in protein transfer and nucleic acid blotting. The papers also provide a uniform flow of buffer through the gel to the transfer membrane in a blotting sandwich.

Blot absorbent filter papers are available in a range of thicknesses:

  • Extra thick — for Trans-Blot® SD semi-dry or Trans-Blot transfer cells (precut to gel dimensions from well to bottom of gel)
  • Thick — for the Mini Trans-Blot cell, Criterion™ blotter, and Bio-Dot® and Bio-Dot SF microfiltration apparatus (applications requiring thick, high wet–strength filter paper)
  • Thin — for transfer of thick gels using wet blotting methods (applications requiring thin, high wet–strength filter paper)

Features and Benefits

  • Cotton fiber papers are used with PVDF or nitrocellulose membranes for the transfer of both proteins and nucleic acids
  • Compatible with all standard immunoblotting and nucleic acid detection methods
  • Extra thick and thick blotting papers are precut for specific gel sizes to fit in all Bio-Rad blotting cells
  • Available in three formats:
    • Convenient precut sheets of PVDF or nitrocellulose membrane/blot absorbant filter paper sandwiches
    • Packs of precut filter paper–only sheets
    • Economical rolls (thin paper only)

Applications

  • Protein transfer from SDS-PAGE gels to membrane in preparation for western blotting
  • Nucleic acid transfer in preparation for northern or Southern blotting

See the Blotting Membrane and Filter Paper Selection Guide for more information on the different filter paper types and size selections by gel type.

Extra Thick Blot Paper #170-3965
Extra Thick Blot Paper

170-3965
Pkg of 60 sheets, 7.5 x 10 cm, absorbent filter paper, for use with Mini-PROTEAN® or Ready Gel® precast gels, precut to gel dimensions

List Price:   $66.00
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Extra Thick Blot Paper #170-3966
Extra Thick Blot Paper

170-3966
Pkg of 60 sheets, 7 x 8.4 cm, absorbent filter paper, for use with Mini-PROTEAN® or Ready Gel® precast gels, precut to gel dimensions

List Price:   $66.00
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Extra Thick Blot Paper #170-3967
Extra Thick Blot Paper

170-3967
Pkg of 60 sheets, 8 x 13.5 cm, absorbent filter paper, for use with Criterion precast gels

List Price:   $66.00
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Extra Thick Blot Paper #170-3968
Extra Thick Blot Paper

170-3968
Pkg of 30 sheets, 14 x 16 cm, absorbent filter paper, for use with PROTEAN II xi gels

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Extra Thick Blot Paper #170-3969
Extra Thick Blot Paper

170-3969
Pkg of 30 sheets, 19 x 18.5 cm, for use with PROTEAN II XL gels

List Price:   $73.00
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Extra Thick Blot Paper #170-3958
Extra Thick Blot Paper

170-3958
Pkg of 30 sheets, 10 x 15 cm, blot absorbent filter paper

List Price:   $66.00
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Extra Thick Blot Paper #170-3959
Extra Thick Blot Paper

170-3959
Pkg of 30 sheets, 15 x 15 cm, absorbent filter paper

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Extra Thick Blot Paper #170-3960
Extra Thick Blot Paper

170-3960
Pkg of 30 sheets, 15 x 20 cm, absorbent filter paper

List Price:   $73.00
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Thick Blot Paper #170-3955
Thick Blot Paper

170-3955
Pkg of 25 sheets, 14 x 16 cm, absorbent filter paper, for use with PROTEAN II xi gels

List Price:   $59.00
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Thick Blot Paper #170-3956
Thick Blot Paper

170-3956
Pkg of 25 sheets, 15 x 20 cm, absorbent filter paper, for use with Trans-Blot cassette (#170-3913)

List Price:   $57.00
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Thick Blot Paper #170-3932
Thick Blot Paper

170-3932
Pkg of 50, 7.5 x 10 cm, absorbent filter paper, for use with Mini Trans-Blot cassette (#170-3931)

List Price:   $57.00
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Thick Blot Paper #165-0921
Thick Blot Paper

165-0921
Pkg of 25, 18 x 34 cm sheets, membrane backing, for Model 224, 443, and 543 slab gel dryers

List Price:   $83.00
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Thick Blot Paper #170-4085
Thick Blot Paper

170-4085
Pkg of 50 sheets, 9.5 x 15.2 cm, absorbent filter paper, for use with the Criterion blotter

List Price:   $61.00
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Bio-Dot/Bio-Dot SF Filter Paper #162-0161
Bio-Dot/Bio-Dot SF Filter Paper

162-0161
Pkg of 60, 11.3 x 7.7 cm, precut absorbent filter paper for blotting applications requiring thick, high-wet-strength filter paper

List Price:   $51.00
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Filter Paper Backing #165-0962
Filter Paper Backing

165-0962
Pkg of 25, 35 x 45 cm paper sheets, for backing stained gels

List Price:   $143.00
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Thin Blot Paper #162-0118
Thin Blot Paper

162-0118
Pkg of 1 roll, 33 cm x 3 m, bulk absorbent filter paper for blotting applications requiring thin, high-wet-strength filter paper

List Price:   $51.00
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