BioLogic DuoFlow 10 System

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BioFrac Fraction Collector
BioFrac Fraction Collector

The BioFrac fraction collector can be used for basic or complex fraction collection schemes at flow rates <100 mL/min. Available stand-alone or with the BioLogic DuoFlow and LP systems, this fraction collector offers flexibility with numerous collection rack options ranging from an icebath/microplate rack to a prep-20 preparative scale rack for large volume collection. Collection in time, drop, or volume mode with peak detection, time windows, or a combination of both.

BioLogic Maximizer
BioLogic Maximizer

The BioLogic Maximizer expands the capabilities of the BioLogic DuoFlow systems. It enables buffer blending, doubled flow rates, and twice the valve capacity of the standard BioLogic DuoFlow system.

BioLogic QuadTec UV/Vis Detector
BioLogic QuadTec UV/Vis Detector

This detector enables simultaneous monitoring of four wavelenghts (190–370 nm) for greater sensitivity without background interference. It is available as a stand-alone unit, as an upgrade to existing BioLogic DuoFlow systems, and as part of BioLogic DuoFlow QuadTec™ and BioLogic DuoFlow Pathfinder® systems.

BioLogic DuoFlow pH Monitor
BioLogic DuoFlow pH Monitor

This pH monitor enables real-time monitoring of pH conditions (pH 1–14) during a sample run. It consists of high-precision calomel electrodes which are compatible with buffers such as Tris that are incompatible with Ag/AgCl electrodes. It is a biocompatible PEEK flow cell with a swept volume of approx 80 µL that is capable of withstanding flow rates to 80 mL/min. The monitor is included with all BioLogic Maximizer™ and Pathfinder® systems and is available as an option on the BioLogic DuoFlow™ and BioLogic DuoFlow QuadTec™ systems, where it connects via the Signal Import Module (SIM).

BioLogic Signal Import Module (SIM)
BioLogic Signal Import Module (SIM)

This optional module allows import of an analog signal (up to 2.5V) from a pH electrode or other external detector (such as a UV, refractive index, or fluorescence monitor). The BioLogic DuoFlow software accommodates two SIMs and can display four data signals simultaneously.

BioLogic DuoFlow Valves
BioLogic DuoFlow Valves

High- and low-pressure valves are available. High-pressure sample injection (AVR7-3) and stream select (AVR9-8) valves use the patented MBB (make-before-break) design that prevents pressure spikes when the valve rotates from one port to another, eliminating baseline interference. The feature is beneficial when using fragile low-pressure columns or flow-sensitive detectors and prevents pump shutdown due to transient pressure conditions.

EZLogic Integration Software
EZ Logic Integration Software

EZLogic integration software adds quantitative functions to basic BioLogic DuoFlow software with the same easy-to-use graphical interface. The software provides tools necessary to quantitate samples, integrate and overlay chromatograms, determine retention times, view results, and generate customized reports. Graphical capabilities include split-screen chromatogram views, zooming, multiple parameter annotation, and color selection.

BioLogic DuoFlow System Selection Guide
BioLogic DuoFlow System Selection Guide
BioLogic DuoFlow 10 system
10 ml/min flow rate to 3,500 psi
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BioLogic DuoFlow 40 system
40 ml/min flow rate to 1,000 psi
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BioLogic DuoFlow QuadTec 10 system
10 ml/min flow rate to 3,500 psi
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BioLogic DuoFlow QuadTec 40 system
40 ml/min flow rate to 1,000 psi
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BioLogic DuoFlow Maximizer 20 system
20 ml/min flow rate to 3,500 psi
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BioLogic DuoFlow Maximizer 80 system
80 ml/min flow rate to 1,000 psi
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BioLogic DuoFlow Pathfinder 20 system
20 ml/min flow rate to 3,500 psi
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BioLogic DuoFlow Pathfinder 80 system
80 ml/min flow rate to 1,000 psi
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• Included as standard. º Option or upgrade.  

The BioLogic DuoFlow™ family of chromatography systems offers flexibility with multiple system configurations, many optional upgrades, and a common software platform that is intuitive and easy to follow. These systems can be used on the laboratory bench or in a coldroom.

The BioLogic DuoFlow 10 system includes an F10 workstation for a flow rate of 0.01–10 ml/min* at 3,500 psi (233 bar, 23 MPa). A Model MX-1 mixer, 3-tray rack, AVR7-3 sample inject valve, fittings kit, UV detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, UNO® Q1 column, and instructions are also included.

A Dell PC controller enables easy communication with the workstation and peripheral devices via an external USB Bitbus communicator. The controller includes the Windows XP operating system, application software, keyboard, mouse, and high-resolution flat-panel monitor.

BioLogic DuoFlow modular components allow the system to meet both laboratory space and application requirements; as requirements change, systems may be easily reconfigured and seamlessly upgraded with increased functionality, such as higher flow rates, sophisticated detection capabilities, pH monitoring, column scouting, and buffer blending.

* To double the flow rate to up to 20 ml/min, use the BioLogic DuoFlow Maximizer™ valve system. The optional F40 pump kit expands pumping capabilities to 40 ml/min.

Operating Limits  
Flow rate 0.01–10 ml/min
Pressure 3,500 psi (23 MPa) at 10 ml/min
Detection  
UV detection Standard 254 and 280 nm filters; optional 214, 365, 405, and 436 nm filters; standards 5 mm analytical flow cell; optional 2 mm preparative flow cell; optional BioLogic QuadTec UV/Vis detector for simultaneous 4-wavelength detection
BioLogic DuoFlow 10 System

760-0037
100/120 V, medium-pressure chromatography system, includes Dell controller and monitor, F10 workstation, mixer, AVR7-3 valve, UV detector with flow cell and filters, conductivity monitor, starter kit

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BioLogic DuoFlow 10 System With BioFrac Fraction Collector

760-0047
100/120 V, medium-pressure chromatography system, includes fraction collector, Dell controller and monitor, F10 workstation, AVR7-3 valve, UV detector with flow cell and filters, conductivity monitor

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Number Description Options
4106040 Instruction Manual, V9-8 Manual, and AV9-8 Automated Stream Select Valves, Rev C Click to download
6439 Improving Aggregate Removal from Monoclonal Antibody Feed Using High Resolution Cation Exchange Chromatography, Rev A Click to download
DC7602030 Declaration of Conformity, BioLogic USB Bitbus Device, Rev A Click to download
2780 Purification of Murine IgG1 Using UNOsphere S and CHT Ceramic Hydroxyapatite Chromatography, Rev A Click to download [ Add to Cart (Free) ]
2849 Protein A Removal From IgG on CHT Ceramic Hydroxyapatite Support, Rev A Click to download
5342 Automated Three-Dimensional Purification Method for Histidine-Tagged Proteins Using the BioLogic DuoFlow Maximizer Chromatography System, Rev A Click to download
5334 A Method for Rapid, Large-Scale Removal of Albumin and IgG From Human Serum Using the BioLogic DuoFlow Chromatography System, Rev A Click to download
5251 Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins, Rev A Click to download
3039 Separation Reproducibility With the BioLogic Chromatography Systems Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download
5369 Biologic DuoFlow System Family Brochure, Rev A Click to download [ Add to Cart (Free) ]
2584 BioLogic DuoFlow Valving Guide, Rev E Click to download
2815 BioLogic DuoFlow Valve Brochure, Rev C Click to download
2687 BioLogic DuoFlow Chromatography System Brochure, Rev D Click to download [ Add to Cart (Free) ]
4106041 Instruction Manual, BioLogic HR Valve Plumbing Diagrams, V7-3, AV7-3, Rev A Click to download
4106050 Instruction Manual, BioLogic HR Sample Inject Port Installation, V7-3, AV7-3 Valves, Rev B Click to download
4106054 Instruction Manual, BioLogic™ HR Gradient Pump Maintenance Kit, Rev C Click to download
4106248 Instruction Manual, BioLogic DuoFlow Chromatography System Software Version 5.0, Rev A Click to download
4006210 Instruction Manual, BioLogic™ Chromatography System Click to download
4106523B Instruction Manual, BioLogic DuoFlow USB Bitbus Communicator, Rev B Click to download
4106171 Instruction Manual, BioLogic DuoFlow Software Version 3.0 Discrepancies Solutions, Rev A Click to download
4106181 Instruction Manual, BioLogic DuoFlow Chromatography System Version 4.0 Software New Features, Rev A Click to download
4006208 Instruction Manual, BioLogic DuoFlow Chromatography System Starter Kit, Rev B Click to download
4106249 Instruction Manual, BioLogic DuoFlow Chromatography System 5.0 Software New Features, Rev A Click to download
4106039 Instruction Manual, BioLogic Column Rack Expansion Kit, Rev A Click to download
4106525 Instruction Manual Insert, BioLogic pH Monitor, Rev B Click to download
4106523 Instruction Manual Insert, BioLogic DuoFlow System USB Bitbus Communicator, Rev B Click to download
4106520 Instruction Manual Insert, BioLogic DuoFlow F10 Tubing Kit, Rev C Click to download
4006229 Instruction Manual, BioLogic DuoFlow™ Chromatography System, Rev B Click to download