Bio-Rad's Urinary Metanephrines by HPLC test is designed for the quantitative determination of metanephrine and normetanephrine in urine. These analytes are used in the diagnosis of pheochromocytoma and related neurogenic tumors. This test does not require manual pH check of samples. It is available for both manual methods and automated methods (on Gilson ASPEC™). In addition, this application is easily compatible with Bio-Rad's Urinary Catecholamines by HPLC assay, since it uses the same analytical column and mobile phase.
The automated application, which is designed for use with Gilson ASPEC™ XL or GX-271, offers high specimen through-put and reduced hands-on time, with an overnight processing option.
Bio-Rad has developed automated protocols for many of our HPLC kits with Gilson ASPEC™ XL or GX-271. Each protocol is validated for complete confidence in the results. These methods help reduce laboratory costs by dramatically decreasing labor hours. Contact your local Bio-Rad representative for more information about Gilson ASPEC™ methods.
- Principle: Isocratic HPLC method (Reversed Phase)
- Duration of chromatography: 16 minutes
- Sample volume: 2 mL urine (manual method), 500 µL urine (automated method)
- Flow rate: 0.7 mL/min
- Electrochemical detection: 650 mV