Bio-Scale™ Mini CFT™ Ceramic Fluoroapatite Cartridges

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Overview

Bio-Scale Mini Cartridge Specifications
Sizes 1 ml and 5 ml bed volumes; desalting cartridges: 10 ml and 50 ml bed volumes
Recommended flow rate Varies for each support type*
Maximum operating pressure 1, 5, and 10 ml: 45 psi
50 ml: 40 psi
Fittings Female luer inlet and male luer outlet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Dimensions 1 ml–40 mm length x 5.6 mm inner diameter
5 ml–40 mm length x 12.6 mm inner diameter
10 ml–80 mm length x 12.6 mm inner diameter**
50 ml–80 mm length x 28.2 mm inner diameter**

* Recommended flow rates are given for each support type in the instruction manual.
** Available for desalting cartridges only.

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732-4120 732-4122 732-4124
UNOsphere™ Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 732-4100 732-4102 732-4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732-4110 732-4112 732-4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732-4140 732-4142 732-4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732-4130 732-4132 732-4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732-4632 732-4634
Antibody Purification
Protein A Affi-Prep® Protein A Affinity Recombinant protein A 30 mg/ml human IgG
732-4200, 1 x 1 ml 732-4201, 5 x 1 ml 732-4202, 1 x 5 ml
Protein A Affi-Prep® Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600 732-4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 732-4322 732-4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 732-4332 732-4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
780-0811 780-0812
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732-4610 732-4612 732-4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732-4642 732-4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732-4502 732-4504 732-5304 732-5312 732-5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Monoclonal Antibody Workflow

Workflow for mAb purification with UNOsphere SUPrA affinity, UNOsphere Q, and CHT Type 1 media.

To read more click here

CFT Specifications
  Type I Type II
Functional groups Ca2+, PO43-, F- Ca2+, PO43-, F-
Observed dynamic binding capacity bovine lgG 14–17 mg/g CFT 30–32 mg/g CFT
Observed dynamic binding capacity lysozyme (Lys) ≥25 mg/g CFT 17–21 mg/g CFT
Nominal pore diameter 600–800 A 600–800 A
Maximum backpressure 55 bar (800 psi) 55 bar (800 psi)
Nominal mean particle size 40 ± 4 µm 40 ± 4 µm
CFT Characteristics
  Types I and II
Typical linear flow rate range 50–300 cm/hr
pH Stability 5–14 pH
Base Stability  
Regeneration Normal — 400 mM sodium phosphate, pH6.8
Difficult — 400 mM trisodium phosphate, pH 11–12
Basic proteins (pl >7)– 1–2 M NaCl or KCI,pH 10–12
Sanitation Perform CIP, then 3–5 CV 1–2 N NaOH or KOH;
exposure time ≥1 hr
Recommended column storage 0.1–1 N NaOH or KOH
Shelf life (dry, unused material) >2 years

* 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5.
** 40 µm particles, 300 cm/hr, 5 mM sodium phosphate, pH 6.5.

Bio-Scale Mini CFT ceramic fluoroapatite cartridges are 5 ml cartridges filled with CFT ceramic fluoroapatite support. CFT ceramic fluoroapatite (Ca10(PO4)6F2) is a rigid, spherical macroporous support used in the purification of biologically active compounds. CFT is a composite of fluoroapatite and hydroxyapatite prepared by chemically converting hydroxyapatite nanocrystals to fluoroapatite with a fluorine reagent. CFT can be used under stringent chromatography conditions to separate acidic proteins requiring buffered conditions as low as pH 5.6. The cartridges can be used for method scouting or simple purifications.

CFT has high binding capacity and may be used reproducibly over an extended number of chromatography runs. Its increased tensile strength, chemical durability, and density provide excellent throughput and consistent performance for all separations, including biopharmaceutical process-scale manufacturing.

Features of CFT include:

  • High binding capacity
  • Sintering at 700°C (Type II) for heavy-duty, durable supports
  • Rigid particles for fast cleaning and equilibration
  • Inorganic calcium phosphate for distinct selectivities

 

Bio-Scale Mini cartridges have a double-wall cartridge design* that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. Convenient, ready to use, and disposable, the cartridges have chemically compatible polypropylene parts. Bio-Scale Mini cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system; simple luer fittings make connection to any system easy (see bulletin 5326).

*U.S. patent 7,208,087.

Bio-Scale Mini CFT Type II cartridges, 1 x 5 ml

732-4405
5 ml, prepacked, CFT ceramic fluoroapatite multimodal chromatography media cartridge, Type II

List Price:   $179.00
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Bio-Scale Mini CFT Type II cartridges, 5 x 5 ml

732-4406
Pkg of 5, 5 ml, prepacked, CFT ceramic fluoroapatite multimodal chromatography media cartridge, Type II

List Price:   $719.00
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Number Description Options
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
5326 Chromatography Column Connection Guide, Rev A Click to download
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download