Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges

Print

Overview

Prepacked Chromatography Cartridge Selection Guide
Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers
by Cartridge Size
5 x 1 ml 1 x 5 ml 5 x 5 ml
Protein and Plasmid Purification
High Q Macro-Prep® High Q Strong anion exchange –N+(CH3)3 ≥37 mg BSA 732-4120 732-4122 732-4124
UNOsphere™ Q UNOsphere Q Strong anion exchange –N+(CH3)3 180 mg BSA 732-4100 732-4102 732-4104
UNOsphere S UNOsphere S Strong cation exchange –SO3- 60 mg human lgG 732-4110 732-4112 732-4114
DEAE Macro-Prep DEAE Weak anion exchange –HN+(C2H5)2 >35 mg protein 732-4140 732-4142 732-4144
Protein Purification
High S Macro-Prep High S Strong cation exchange –SO3- ≥49 mg human lgG 732-4130 732-4132 732-4134
IgG Purification
DEAE Blue DEAE Affi-Gel® Blue Affinity

Cibacron Blue

F3GA and DEAE

0.2–1.0 ml serum

732-4632 732-4634
Antibody Purification
Protein A Affi-Prep® Protein A Affinity Recombinant protein A 30 mg/ml human IgG
732-4200, 1 x 1 ml 732-4201, 5 x 1 ml 732-4202, 1 x 5 ml
Protein A Affi-Prep® Protein A Affinity Protein A 8–10 mg mouse
monoclonal lgG1;
16–23 mg human lgG
732-4600 732-4602
CHT™ Type I CHT ceramic
hydroxyapatite Type I, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 25–60 mg human lgG 732-4322 732-4324
CHT Type II CHT ceramic
hydroxyapatite
Type II, 40 µm
Hydroxyapatite (Ca5(PO4)3OH)2 15–25 mg human lgG 732-4332 732-4334
Affinity-Tagged Protein Purification
IMAC** Nuvia™ IMAC Affinity Nitrilotriacetic acid ≥40 mg recombinant
histidine-tagged protein
780-0811 780-0812
IMAC** Profinity™ IMAC Affinity Iminodiacetic acid ≥15 mg recombinant
Histidine-tagged protein
732-4610 732-4612 732-4614
GST** Profinity GST Affinity Glutathione ≥10 mg/ml recombinant
GST-tagged protein
732-4620 732-4622 732-4624
Purification of Serum Proteins, Enzymes
Blue Affi-Gel Blue Affinity Cibacron Blue
F3GA
0.2 ml serum
11 mg albumin
732-4642 732-4644

Cartridge by Application Support Type of Separation Chemical Form Protein Capacity/ml* Catalog Numbers by Cartridge Size
1 x 5
ml
5 x 5
ml
5 x 10
ml
1 x 50
ml
5 x 50
ml
Desalting, Buffer Exchange
P-6 Bio-Gel® P-6 Gel filtration Polyacrylamide 10–22% CV*** 732-4502 732-4504 732-5304 732-5312 732-5314

* Protein capacity based on bulk support; check individual instruction manuals for run conditions and specifications.
** IMAC and GST cartridges are designed for use with the Profinia Protein Purification System.
*** Column volume.

PDF Bulletin 5584 Download the Bio-Scale Mini Cartridges Product Information Sheet for more information.

Size Exclusion Information

Size Exclusion Chromatography

Size exclusion chromatography, also known as gel filtration chromatography, separates molecules by size. The gel matrix consists of spherical beads with pores of a specific size distribution. Separation occurs when molecules of different sizes are excluded from the pores. As small molecules diffuse into the pores, their flow through the column is retarded, while large molecules bypass the pores and are eluted in the column’s void volume. Consequently, molecules are separated according to size and elute in order of decreasing molecular weight.

Operating conditions and selection of media depend on the application and the desired resolution. A common method used in size exclusion chromatography is group separation, which includes desalting and fractionation.
Desalting — The molecule of interest is eluted in the void volume, while smaller molecules are retained in the gel pores; to achieve the desired separation, the matrix should have an exclusion limit smaller than the molecule of interest.
Fractionation — Molecules with a range of molecular weights are separated within the gel matrix; with this method, the molecules of interest should fall within the fractionation range of the gel.

Common applications include protein fractionation and molecular weight determination, nucleic acid separation, plasmid purification, and polysaccharide fractionation.

Resolution depends on particle size, pore size, flow rate, column dimensions, and sample volume. Generally, the highest resolution is obtained with low flow rates (2–10 cm/hr), long, narrow columns, small-particle-size gels, small sample volumes (1–5% of the total bed volume), 2-fold difference in molecular weights, and a sample viscosity that is the same as the eluent. For desalting, the sample volume can be as much as 30–40% of the total bed volume, and shorter, wider columns may be used.

For a list of antibody purification applications, request bulletin 1801. Protein applications are given in bulletin 1802.

Bio-Gel P Polyacrylamide Gels

Bio-Gel P polyacrylamide gels, for high-resolution gel filtration, are prepared by copolymerization of acrylamide and N,N'-methylene-bis-acrylamide. Bio-Gel P gels:

  • Are available in several particle size ranges with molecular weight exclusion limits ranging from 100 to 100,000
  • Are extremely hydrophilic and essentially nonionic
  • Provide efficient, gentle gel filtration of sensitive compounds
  • Do not support microbial growth or leach carbohydrates (due to their synthetic composition), as dextrose and agarose gels can

Bio-Gel P gels are autoclavable at pH 5.5–6.5 and operate over a pH range of 2–10 at room temperature. Flow rate and resolution increase with increasing temperature in the range of 4–80°C.

The gels are compatible with dilute organic acids, 8 M urea, chaotropic agents, detergents, and miscible organic solvents. Alcohol, up to 20% v/v, can be used to enhance the solubility of nucleotides, peptides, and tannins without altering the exclusion properties of the gels. Formamide may also be used at full strength since Bio-Gel P gels are completely swollen in this solvent.

Bio-Gel P-6DG gel, with a molecular weight exclusion limit of 6,000, is recommended specifically for desalting and buffer exchange applications. It provides rapid results with high sample recovery and minimal sample dilution. Its hydrophilicity ensures little or no interaction between the gel and sample molecules.

For more information, request bulletin 2068.

Properties
 
Hydrated Bead
Size (µm)
Hydrated Bed
Volume Mass Dry Gel
Typical Flow
Rates (cm/hr)*
Molecular Weight
Fractionation Range
Bio-Gel P-2 gel, fine
Bio-Gel P-2 gel, extra fine
45–90
<45
3 ml/g
5–10
<10
100–1,800
Bio-Gel P-4 gel, medium
Bio-Gel P-4 gel, fine
Bio-Gel P-4 gel, extra fine
90–180
45–90
<45
4 ml/g
15–20
10–15
<10
800–4,000
Bio-Gel P-6 gel, medium
Bio-Gel P-6 gel, fine
Bio-Gel P-6 gel, extra fine
Bio-Gel P-6DG gel
90–180
45–90
<45
90–180
6.5 ml/g
15–20
10–15
<10
15–20
1,000–6,000
Bio-Gel P-10 gel, medium
Bio-Gel P-10 gel, fine
90–180
45–90
7.5 ml/g
15–20
10–15
1,500–20,000
Bio-Gel P-30 gel, medium
Bio-Gel P-30 gel, fine
90–180
45–90
9 ml/g
7–13
6–11
2,500–40,000
Bio-Gel P-60 gel, medium
Bio-Gel P-60 gel, fine
90–180
45–90
11 ml/g
4–6
3–5
3,000–60,000
Bio-Gel P-100 gel, medium
Bio-Gel P-100 gel, fine
90–180
45–90
12 ml/g
4–6
3–5
50,000–100,000
* Flow rates determined in a 1.5 x 70 cm column using a hydrostatic pressure head-to-bed ratio of 1:1.

Bio-Scale Mini cartridges filled with Bio-Gel P-6 gel filtration (size exclusion) chromatography support are designed for direct connection to BioLogic™ LP chromatography systems and the Profinia™ protein purification system. Luer fittings enable easy connection to any chromatography system, a peristaltic pump, or a syringe for simple step elution.

Features and Benefits

  • Ideal for desalting or buffer exchange
  • Convenient, ready-to-use, disposable cartridges — 5 ml, 10 ml, or 50 ml
  • Double-walled cartridge design* provides extra durability for runs at pressures up to 45 psi
  • Polypropylene construction compatible with aqueous buffers commonly used for protein separation

More Information

High Performance Columns For Antibody and Tagged Protein PurificationsLearn more about Bio-Rad’s suite of high-performance chromatography columns designed for high-resolution laboratory scale protein purification.







Related Products

Fitting adaptors are available for connection to other low- and medium-pressure chromatography systems:

  • Fittings kit for connection to BioLogic DuoFlow™ systems (732-0113)
  • Fittings kit for connection to older AKTA systems with M6 fittings (732-0111)
  • Fittings kit for connection to NGC™ systems and newer AKTA systems with 1/16th inch/10-32 fittings (732-0112)

Bio-Rad chromatography systems:

 * U.S.patent 7,208,087.

Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 5 ml

732-4502
Pkg of 1, 5 ml, prepacked gel filtration column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price:   $75.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 5 ml

732-4504
Pkg of 5, 5 ml, prepacked gel filtration column, 40 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price:   $236.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 10 ml

732-5304
Pkg of 5, 10 ml, prepacked gel filtration column, 80 mm x 12.6 mm, max. pressure 45 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price:   $435.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridge, 50 ml

732-5312
Pkg of 1, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price:   $429.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Bio-Scale™ Mini Bio-Gel® P-6 Desalting Cartridges, 50 ml

732-5314
Pkg of 5, 50 ml, prepacked gel filtration column, 80 mm x 28.2 mm, max. pressure 40 psi, for use with BioLogic™, Profinia™ and other chromatography systems

List Price:   $1,748.00
Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
6498 Lab-Scale Protein Purification – Column and Cartridge Selection Guide, Rev C Click to download [ Add to Cart (Free) ]
5742 Profinity eXact Purification Methods on the Profinia System, Rev. A Click to download [ Add to Cart (Free) ]
5725 Profinia Buffers for Profinity eXact Chromatography Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5712 The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A Click to download [ Add to Cart (Free) ]
5514 Automated Purification of a His-Tagged Protein With the Profinia Protein Purification System: Comparison With Another Low-Pressure Chromatography System, Rev A Click to download [ Add to Cart (Free) ]
10006995 Bio-Scale Mini Bio-Gel® P-6 Desalting Cartridges, 10 and 50 ml, Instruction Manual, Rev C Click to download
5925 Affinity Purification Buffer Kit Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
6484 High-Performance Columns for Antibody and Tagged Protein Purifications Product Information Sheet, Rev B Click to download