Bio-Plex Pro™ Mouse Cytokine, Chemokine, and Growth Factor Assays

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Bio-Plex Pro Mouse Cytokine, Chemokine, and Growth Factor Assays

Overview

Available Bio-Plex Pro™ Mouse Cytokine & Chemokine Assays
Singleplex Bio-Plex Pro Mouse Chemokine Panel 33-Plex
(12002231)
Bio-Plex Pro Mouse Chemokine MCP-2 / CCL8 Singleplex
(12002247)
Bead Regions*
BCA-1/CXCL13   28
CTACK/CCL27   62
ENA-78/CXCL5   20
Eotaxin/CCL11   74
Eotaxin2/CCL24   73
Fractalkine/CX3CL1   25
GM-CSF   22
I-309/CCL1   26
IFN-γ   34
IL-1β   19
IL-2   36
IL-4   39
IL-6   38
IL-10   56
IL-16   29
IP-10/CXCL10   35
I-TAC/CXCL11   37
KC/CXCL1   57
MCP-1/CCL2   51
MCP-2/CCL8**   42
MCP-3/CCL7   46
MCP-5/CCL12   48
MDC/CCL22   52
MIP-1α/CCL3   77
MIP-1β/CCL4   75
MIP-2/CXCL2   27
MIP-3α/CCL20   12
MIP-3β/CCL19   64
RANTES/CCL5   55
SCYB16/CXCL16   65
SDF-1α/CXCL12   67
TARC/CCL17   63
TECK/CCL25   78
TNF-α   21

Mouse
Cytokine
Group I
8-Plex
Panel
(M60
000007A
)
23-Plex
Panel
(M60
009RDPD
)
Th1/Th2
Panel
(M60
00003J7
)
Th1
Panel
(L60
00004C6
)
Th2
Panel
(L60
000UKVT
)
Th17
6-Plex
Panel
(M60
00007NY
)
Singleplex Bead Regions*
IL-1α        
171G5001M 53
IL-1β     171G5002M 19
IL-2   171G5003M 36
IL-3           171G5004M 18
IL-4     171G5005M 39
IL-5     171G5006M 52
IL-6     171G5007M 38
IL-9         171G5008M 33
IL-10 171G5009M 56
IL-12 p40           171G5010M 76
IL-12 p70       171G5011M 78
IL-13         171G5012M 37
IL-17A         171G5013M 72
Eotaxin           171G5014M 74
G-CSF           171G5015M 54
GM-CSF       171G5016M 73
IFN-γ  •   171G5017M 34
KC           171G5018M 57
MCP-1 (MCAF)           171G5019M 51
MIP-1α           171G5020M 77
MIP-1β           171G5021M 75
RANTES           171G5022M 55
TNF-α   171G5023M 21

Mouse Cytokine Group II 9-Plex Panel
(MD000000EL)
Singleplex Bead Regions*
IL-15 171G6001M 42
IL-18***   20
Basic FGF 171G6002M 25
LIF 171G6003M 45
M-CSF 171G6004M 26
MIG 171G6005M 44
MIP-2 171G6006M 27
PDGF-BB 171G6007M 35
VEGF 171G6008M 47

 
TGF-β Group 3-Plex Panel
(171W4001M)
Singleplex Bead Regions
TGF-β1 171V4001M 13
TGF-β2 171V4002M 72
TGF-β3 171V4003M 66

1. * You can design an x-Plex assay or Express assay with any combination of analytes within a group, except for ICAM-1.

2. ** MCP-2/CCL8 requires higher sample dilution and cannot be mixed with other targets. Complete kit 12002231.

3. *** IL-18 is not available as a singleplex assay but only as part of an assay panel.

Bio-Plex Pro™ Mouse Cytokine Assay Specifications

Representative working assay range*, sensitivity, and precision**. Values were experimentally determined from five assay runs.

  Assay Working Ranges pg/ml Assay Sensitivity pg/ml Assay Precision
Targets LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV
Mouse Group I: 23-plex panel
IL-1α 1.84 21,093 0.2 3 29
IL-1β 10.36 60,631 9.4 4 7
IL-2 3.72 51,857 0.6 3 19
IL-3 1.55 21,632 0.2 2 15
IL-4 6.98 9,372 2.1 3 20
IL-5 3.57 13,315 0.3 4 14
IL-6 0.74 12,053 0.2 3 16
IL-9 6.89 28,208 12.5 4 9
IL-10 2.95 12,066 1.0 4 5
IL-12 (p40) 1.53 25,024 0.4 2 7
IL-12 (p70) 1.62 26,507 2.3 3 7
IL-13 47.2 57,011 38.7 4 5
IL-17A 2.65 43,337 0.8 3 10
Eotaxin 257.9 4,636 147.4 4 5
G-CSF 5.1 84,244 0.6 3 12
GM-CSF 21.2 3,401 5.6 3 24
IFN-γ 1.84 30,164 1.2 4 6
KC 3.2 18,202 0.3 3 30
MCP-1 22.4 41,873 3.7 5 7
MIP-1α 256.2 15,565 36.3 3 11
MIP-1β 3.33 24,798 0.8 3 8
RANTES 2.78 8,759 0.6 4 4
TNF-α 5.8 59,626 1.4 3 6
Mouse Group II: 9-plex panel
IL-15 5.7 37,501 6.6 6 3
IL-18 81.4 29,761 31.8 8 18
Basic FGF 4.8 35,500 2.2 8 8
LIF 3.5 57,366 0.6 4 12
M-CSF 1.5 24,221 0.4 4 14
MIG 183 46,393 3.4 5 21
MIP-2 32 8,574.2 0.4 5 13
PDGF-BB 10.4 36,939 4.3 5 10
VEGF 27.2 32,771 1.6 4 15

* Assay range is LLOQ and ULOQ calculated from five independent assays.

** Data were generated using vacuum manifold. %CV is expected to be lower with magnetic bead washer.

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is two-fold of the standard deviation above the background signal

Bio-Plex Pro™ Mouse Th17 Assay Specifications
  Assay Working Ranges pg/ml Assay Sensitivity pg/ml Assay Precision
  Target LLOQ ULOQ LOD Intra-Assay %CV Inter-Assay %CV
8-Plex IL-17F 1 6,362 0.1 5 5
IL-21 6 8,207 2.8 3 15
IL-22 0.5 8,433 0.1 4 5
IL-23p19 3.4 55,764 0.7 5 6
IL-31 2.5 41,313 5.2 6 6
IL-33 0.5 7,343 0.2 5 12
CD40L 3.5 11,245 0.4 4 14
MIP-3α 0.7 1,660 0.3 6 13
 
6-Plex IL-1α 10.36 60,631 9.4 4 7
IL-6 0.74 12,053 0.2 3 16
IL-10 2.95 12,066 1 4 5
IL-17A 2.65 43,337 0.8 3 10
IFN-γ 1.84 30,164 1.2 4 6
TNF-α 5.8 59,626 1.4 3 6
 
2-Plex IL-25 168 98,266 9 9 12
IL-27p28 63.4 117,521 29.2 9 6
 
Singleplex ICAM-1 2.9 23,723 0.78 4 8

Assay Performance Definitions

Assay working range — the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ).

Precision — the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy — ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (limit of detection, LOD) — the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal

TGF-β Assay Specifications Overlay
Incubation time
3-Plex Panel
(171-W4001)
Sample volume
12.5 µl
Accuracy (recovery range)
80–100%
Precision
   Intra-assay %CV
   Inter-assay %CV

   ≤10%
   ≤15%


Representative assay working ranges, assay sensitivity, and precision. The LLOQ, ULOQ, LOD, and inter-assay precision %CV are mean data determined from three independent multiplex assays in serum-based matrix. Intra-assay %CV is derived from one representative assay. LLOQ and ULOQ are defined as the boundary standard curve points in which the performance specifications of individual standard points were met for 10% intra-assay CV, 15% inter-assay CV, and recovery range of 80–100%. Data were generated using the magnetic workflow with the Bio-Plex Pro II wash station.

TGF-β Assay Specifications
 
Assay Working Ranges, pg/ml
Assay Sensitivity, pg/ml
Assay Precision
Targets LLOQ ULOQ LOD Intra-assay %CV Inter-assay %CV
Premixed 3-Plex Assay
TGF-β1 1.69  27,616 3.9
4.5
4.9
TGF-β2
14.7 
30,080 1.9
6.3 
9.1
TGF-β3
2.8 
15,031 0.5
6.9 
8.2


Assay Performance Definitions
Assay working range – the range of concentrations within which the assay is precise and accurate. Boundaries of the assay working range are the lower limit of quantitation (LLOQ) and the upper limit of quantitation (ULOQ)

Precision – the coefficient of variation (%CV) at concentrations within the assay working range

Accuracy – ratio of the observed concentration versus the expected concentration of a known amount of spiked analyte within the assay working range

Sensitivity (Limit of Detection, LOD) – the concentration of analyte for which the fluorescence intensity signal is 2-fold of the standard deviation above the background signal.

Bio-Plex Assay Builder

Use the Bio-Plex® Assay Builder to design your customized Bio-Plex Assay in four easy steps

With Assay Builder you can also:

  • Get a price quote
  • Place your order online
  • Look up an existing assay by catalog number

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

Launch Bio-Plex Assay Builder


Bio-Plex® Technology and Applications


Bio-Plex Pro Mouse Cytokine, Chemokine, and Growth Factor Magnetic Bead–Based Assays detect and measure levels of 46 analytes in diverse matrices such as serum, plasma, and tissue culture supernatants. Cytokines, chemokines, and growth factors are cell-signaling proteins that mediate a wide range of physiological responses, including immunity, inflammation, and hematopoiesis. They are associated with a spectrum of diseases ranging from tumor growth, to infections, to Parkinson's disease.

Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

Key Features

  • Magnetic bead–based for simplified plate processing
  • Developed to deliver accurate, reproducible measurements in multiple sample matrices
  • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
  • Available as all-in-one, space-saving complete kit
  • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
  • Compatible with Bio-Plex Pro Mouse Diabetes Assays for an expanded biomarker profile

Flexible Ordering Options

For multiplexing capabilities among cytokines, refer to Bulletin 5927. For multiplexing capabilities with diabetes assays, refer to Bulletin 5975.

Instrument Compatibility

  • Bio-Plex® MAGPIX™ or Luminex MAGPIX System
  • Bio-Plex 200 or Luminex 200 System
  • Bio-Plex 3D or Luminex FLEXMAP 3D System
  • Luminex MAGPIX System
  • Bio-Plex Pro Wash Station
  • Bio-Plex Pro II Wash Station

More Information

See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

Related Products

Bio-Plex Pro™ Human Inflammation Assays
The most popular biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can be measured in a single multiplex kit. Bio-Plex Pro Human Inflammation Assays will be available in larger 24-plex and 37-plex screening panels and a pathway-focused Treg 12-plex panel, along with singleplex and custom-configured assays.

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Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Waste Electrical and Electronic Equipment ("WEEE Directive")

This product contains electrical or electronic material. Learn more about Bio-Rad's EU Recycle Program

Bio-Plex Pro™ Mouse Cytokine Th17 Panel A 6-Plex

M6000007NY
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 6 mouse cytokines all in one kit

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$1,512.00
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Bio-Plex Pro™ Mouse Cytokine Th17 Panel B 8-Plex Group III

171FA001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 mouse cytokines all in one kit (this product may be temporarily unavailable)

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$2,151.00
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Bio-Plex Pro™ Mouse Cytokine Th17 Panel B 7-Plex Group III
Bio-Plex Pro™ Mouse Cytokine Th17 Panel B 7-Plex Group III

LJ00000163
1 x 96-well flat bottom plate, x-Plex™ Assay format that includes premixed coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 7 mouse cytokines all in one kit

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$3,030.00
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Bio-Plex Pro™ Mouse Cytokine Th1 Panel

L6000004C6
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 7 mouse cytokines all in one kit

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$2,856.00
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Bio-Plex Pro™ Mouse Cytokine Th2 Panel

L60000UKVT
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 7 mouse cytokines all in one kit

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$2,856.00
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Bio-Plex Pro Mouse Cytokine 8-plex Assay #M60-000007A
Bio-Plex Pro™ Mouse Cytokine 8-plex Assay

M60000007A
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 mouse cytokines all in one kit

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$1,876.00
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Bio-Plex Pro Mouse Cytokine Th1/Th2 Assay #M60-00003J7
Bio-Plex Pro™ Mouse Cytokine Th1/Th2 Assay

M6000003J7
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 8 mouse cytokines all in one kit

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$1,887.00
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Bio-Plex Pro Mouse Cytokine 23-plex Assay #M60-009RDPD
Bio-Plex Pro™ Mouse Cytokine 23-plex Assay

M60009RDPD
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, reagents, and diluents for detecting 23 mouse cytokines all in one kit

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$4,027.00
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Bio-Plex Pro™ TGF-β 3-plex Assay

171W4001M
1 x 96-well, includes coupled magnetic beads, detection antibodies, standards, buffers, diluents, streptavidin-PE, filter plate, and sealing tape for the detection of TGF-β1, TGF-β2, TGF-β3 all in one kit

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$1,070.00
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Number Description Options
6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download
6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Rev E Click to download [ Add to Cart (Free) ]
5405 Bio-Plex® Multiplex System Brochure, Ver E Click to download [ Add to Cart (Free) ]
6110 Bio-Plex Pro Mouse Th17 Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
M10014905 Instruction Manual, Bio-Plex Pro Cytokine, Chemokine, and Growth Factors Assays, Rev D
10000076327 Bio-Plex Pro™ Mouse and Rat Cytokine Quick Guide, Ver A Click to download
3100 Bio-Plex Cytokine Assay Comparison, Rev E Click to download
5297 Bio-Plex Pro™ Assay Reference Guide, Ver D Click to download
2993 Bio-Plex Glossary, Rev A Click to download
3074 Differential Immune Activation in Astrocytes of IL-6 Genotypes, Rev A Click to download
BR125FS Second Generation Multiplex Immunoassays Click to download
2900 Selection of Standards for Bio-Plex Cytokine Assays, Rev A Click to download
6054 Bio-Plex Pro TGF-beta Assays Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
5975 Multiplexing Compatibility of the Bio-Plex Pro Diabetes and Cytokine Assays, Rev A Click to download
5927 Multiplexing Across Cytokine Panels, Rev A Click to download
5800 Development and Validation of Two Magnetic Bead-Based Mouse Cytokine Multiplex Assay Panels, Rev A Click to download
5827 Bio-Plex Pro Mouse Cytokine, Chemokine, and Growth Factor Assays Product Sheet, Rev C Click to download [ Add to Cart (Free) ]