Bio-Plex Pro™ Human Th17 Cytokine Assays

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Bio-Plex Pro Human Th17 Cytokine Assays - Bio-Plex Pro™ Magnetic Cytokine, Chemokine, and Growth Factor Assays

Overview

Available Human Cytokine and Chemokine Assays
Human Chemokine Analytes 40-plex Panel
(171-AK99MR2)
Singleplex Sets* Bead Region
6Ckine / CCL21 171-BK11MR2 12
BCA-1 / CXCL13 171-BK12MR2 74
CTACK / CCL27 171-BK13MR2 72
ENA-78 / CXCL5 171-BK14MR2 73
Eotaxin / CCL11 171-BK15MR2 43
Eotaxin-2 / CCL24 171-BK16MR2 30
Eotaxin-3 / CCL26 171-BK17MR2 65
Fractalkine / CX3CL1 171-BK18MR2 77
GCP-2 / CXCL6 171-BK19MR2 15
GM-CSF 171-BK21MR2 34
Gro-α / CXCL1 171-BK22MR2 61
Gro-β / CXCL2 171-BK23MR2 78
I-309 / CCL1 171-BK24MR2 20
IFN-γ 171-BK25MR2 21
IL-1β 171-BK26MR2 39
IL-2 171-BK27MR2 38
IL-4 171-BK28MR2 52
IL-6 171-BK29MR2 19
IL-8 / CXCL8 171-BK31MR2 54
IL-10 171-BK32MR2 56
IL-16 171-BK33MR2 27
IP-10 / CXCL10 171-BK34MR2 48
I-TAC / CXCL11 171-BK35MR2 25
MCP-1 / CCL2 171-BK36MR2 53
MCP-2 / CCL8 171-BK37MR2 57
MCP-3 / CCL7 171-BK38MR2 26
MCP-4 / CCL13 171-BK39MR2 28
MDC / CCL22 171-BK41MR2 29
MIF 171-BK42MR2 35
MIG / CXCL9 171-BK43MR2 14
MIP-1α / CCL3 171-BK44MR2 55
MIP-1δ / CCL15 171-BK46MR2 66
MIP-3α / CCL20 171-BK47MR2 62
MIP-3β / CCL19 171-BK48MR2 76
MPIF-1 / CCL23 171-BK49MR2 37
SCYB16 / CXCL16 171-BK51MR2 64
SDF-1α+β / CXCL12 171-BK52MR2 22
TARC / CCL17 171-BK53MR2 67
TECK / CCL25 171-BK54MR2 46
TNF-α 171-BK55MR2 36

Available Human Cytokine Assays

Human Group I
8-Plex Panel (M50-000007A)
17-Plex Panel (M50-00031YV)
27-Plex Panel (M50-0KCAF0Y)
Th1/Th2 Panel (M50-00005L3)
Singleplex/ x-Plex Bead Regions**
IL-1β
 
• 
• 
 
39
IL-1ra    
• 
 
25
IL-2
• 
• 
• 
• 
38
IL-4
• 
• 
• 
• 
52
IL-5  
• 
• 
• 
33
IL-6
• 
• 
• 
 
19
IL-7  
• 
• 
 
74
IL-8
• 
• 
• 
 
54
IL-9    
• 
 
77
IL-10
• 
• 
• 
• 
56
IL-12 p70  
• 
• 
• 
75
IL-13  
• 
• 
• 
51
IL-15    
• 
 
73
IL-17  
• 
• 
 
76
Basic FGF    
• 
 
44
Eotaxin    
• 
 
43
G-CSF  
• 
• 
 
57
GM-CSF
• 
• 
• 
• 
34
IFN-γ
• 
• 
• 
• 
21
IP-10    
• 
 
48
MCP-1 (MCAF)
 
• 
• 
 
53
MIP-1α    
• 
 
55
MIP-1β  
• 
• 
 
18
PDGF-BB    
• 
 
47
RANTES    
• 
 
37
TNF-α
• 
• 
• 
• 
36
VEGF    
• 
 
45

Human Group II
21-Plex Panel
(MF0-005KMII)
Singleplex/ x-Plex Bead Regions**
IL-1α
• 
63
IL-2Rα
• 
13
IL-3
• 
64
IL-12 (p40)
• 
28
IL-16
• 
27
IL-18***
• 
42
CTACK
• 
72
GRO-α
• 
61
HGF
• 
62
ICAM-1
 
12
IFN-α2
• 
20
LIF
• 
29
MCP-3
• 
26
M-CSF
• 
67
MIF
• 
35
MIG
• 
14
β-NGF
• 
46
SCF
• 
65
SCGF-β
• 
78
SDF-1α
• 
22
TNF-β
• 
30
TRAIL
• 
66
V-CAM1  
15

Th17 Assays
15-Plex Panel
(171-AA001M)
Singleplex
Bead Regions
IL-1β
• 
39
IL-4
• 
52
IL-6
• 
19
IL-10
• 
56
IL-17A
• 
76
IL-17F
• 
44
IL-21
• 
47
IL-22
• 
18
IL-23
• 
43
IL-25
• 
55
IL-31
• 
62
IL-33
• 
46
IFN-γ
• 
21
sCD40L
• 
29
TNF-α
• 
36
IL-17A/F  
66

TGF-β Group
3-Plex Panel
(171-W4001M)
Singleplex
Bead Regions
TGF-β1
• 
13
TGF-β2
• 
72
TGF-β3
• 
66

Available Human Inflammation Assays

  Bio-Plex Pro™ Human Inflammation Panel 1, 37-Plex (171-AL001M) Bio-Plex Pro™ Human Inflammation Panel 1, 24-Plex (171-AL002M) Bio-Plex Pro™ Human Treg Cytokine Panel 12-Plex (171-AL003M) Singleplex Sets1
APRIL / TNFSF13   171-BL001M
BAFF / TNFSF13B   171-BL002M
sCD30 / TNFRSF8   171-BL003M
sCD163   171-BL004M
Chitinase-3-like 1   171-BL005M
gp130 / sIL-6Rβ   171-BL006M
IFN-α2     171-BL007M
IFN-β   171-BL008M
IFN-γ     171-BL009M
IL-2   171-BL010M
sIL-6Rα     171-BL011M
IL-8     171-BL012M
IL-10  
171-BL013M
IL-11   171-BL014M
IL-12 (p40)   171-BL015M
IL-12 (p70)   171-BL016M
IL-19 171-BL017M
IL-20 171-BL018M
IL-22   171-BL019M
IL-26 171-BL020M
IL-27 (p28) 171-BL021M
IL-28A / IFN-λ2
171-BL022M
IL-29 / IFN-λ1 171-BL023M
IL-32
171-BL024M
IL-34
171-BL025M
IL-35 171-BL026M
LIGHT / TNFSF14   171-BL027M
MMP-1

171-BL028M
MMP-2
  171-BL029M
MMP-3     171-BL030M
Osteocalcin
  171-BL031M
Osteopontin     171-BL032M
Pentraxin-3   171-BL033M
sTNF-R1   171-BL034M
sTNF-R2   171-BL035M
TSLP   171-BL036M
TWEAK / TNFSF12   171-BL037M

* Require reagent kit III (171-304090M or 171-304090) and standard (171-DK0001).

** Custom assays can be ordered with any combination of analytes within a group, except for ICAM-1 and VCAM-1, which require different sample dilutions. For more information about compatibility between groups see Bulletin 5927.

*** IL-18 is not available as a singleplex assay, only as part of an assay panel.

† IL-17A/F is available only as a singleplex set and cannot be mixed with IL-17A or IL-17F.

‡ TGF-β assays are compatible with human, mouse, and rat samples.

1 Singleplex assays require Reagent Kit III (171-304090M) and a vial of standards (171-DL0001).

Bio-Plex Assay Builder

Use the Bio-Plex® Assay Builder to design your customized Bio-Plex Assay in four easy steps

With Assay Builder you can also:

  • Get a price quote
  • Place your order online
  • Look up an existing assay by catalog number

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

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Bio-Plex® Technology and Applications

The Bio-Plex Pro™ Human Th17 Cytokine Panel is a unique blend of magnetic bead–based immunoassays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway, which is thought to play a key role in inflammatory conditions such as:

  • Rheumatoid arthritis, lupus, psoriasis, multiple sclerosis, and other autoimmune diseases
  • Antimicrobial immunity
  • Transplant rejection
  • Cancer

The panel enables measurement of the following analytes in diverse matrices such as serum, plasma, and cell culture supernatants:

  • IL-1β
  • IL-4
  • lL-6
  • IL-10
  • IL-17A
  • IL-17F
  • IL-17A/F (singleplex only)
  • IL-21
  • IL-22
  • IL-23
  • IL-25
  • IL-31
  • IL-33
  • IFN-γ
  • sCD40L
  • TNF-α
  • Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

    Key Features

    • Reagent kit ll with diluents optimized for serum and plasma samples
    • Assay quick guide to get you started right away
    • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
    • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
    • 2-level quality controls included with premixed kits

    Flexible Ordering Options

    Additional Th17 Pathway Assays:

    • TGF-β 3-plex panel (#171-V4003M)
    • TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-D60001) for a complete assay

    More Information

    See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

    Related Products

    Bio-Plex Pro™ Human Inflammation Assays
    For the first time, the key biomarkers of inflammation from the TNF superfamily proteins, IFN family proteins, Treg cytokines, and MMPs can be measured using a single multiplex kit. Bio-Plex Pro Human Inflammation Assays are available in larger 24-plex and 37-plex screening panels and a pathway-focused Treg 12-plex panel, along with singleplex and custom-configured assays.

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    Bio-Plex Pro™ Human Th17 Cytokine Panel 15-Plex

    171AA001M
    1 x 96-well all-in-one kit, includes coupled magnetic beads, detection antibodies, standards, controls, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 15 human Th17 cytokines

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    Bio-Plex Pro™ Human Th17 Cytokine IL-1β Set

    171BA001M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-1β, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    Bio-Plex Pro™ Human Th17 Cytokine lL-4 Set

    171BA002M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-4, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    Bio-Plex Pro™ Human Th17 Cytokine lL-6 Set

    171BA003M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human lL-6, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    Bio-Plex Pro™ Human Th17 Cytokine IL-10 Set

    171BA004M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-10, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    Bio-Plex Pro™ Human Th17 Cytokine IL-17A Set

    171BA005M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-17F Set

    171BA006M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17F, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-21 Set

    171BA007M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-21, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-22 Set

    171BA008M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-22, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-23 Set

    171BA009M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-23, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-25 Set

    171BA010M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-25, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-31 Set

    171BA011M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-31, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-33 Set

    171BA012M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-33, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    Bio-Plex Pro™ Human Th17 Cytokine IFN-γ Set

    171BA013M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IFN-γ, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    Bio-Plex Pro™ Human Th17 Cytokine sCD40L Set

    171BA014M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sCD40L, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine TNF-α Set

    171BA015M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human TNF-α, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-17A/F Set

    171BA016M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A/F, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

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    $226.00
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    Bio-Plex Pro™ TGF-β1 Set

    171V4001M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β1, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

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    $253.00
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    Bio-Plex Pro™ TGF-β2 Set

    171V4002M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β2, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

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    $253.00
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    Bio-Plex Pro™ TGF-β3 Set

    171V4003M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β3, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

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    $253.00
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    Number Description Options
    6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download [ Add to Cart (Free) ]
    6249 Bio-Plex Pro™ Human Th17 Cytokine Assays, Rev B Click to download [ Add to Cart (Free) ]
    6251 Multiplex Analysis of Inflammatory Markers Using Bio-Plex Pro Human Th17 Cytokine Assays, Rev A Click to download [ Add to Cart (Free) ]
    10022581 Instruction Manual, Bio-Plex Pro Assays Quick Guide, Rev C Click to download
    10023381 Instruction Manual, Bio-Plex Pro™ Human Th17 Cytokine Assays, Rev A Click to download
    6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
    6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download