Bio-Plex Pro™ Human Th17 Cytokine Assays

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Bio-Plex Pro Human Th17 Cytokine Assays - Bio-Plex Pro Human Th17 Cytokine Assays - Bio-Plex Pro Magnetic Cytokine, Chemokine, and Growth Factor Assays

Overview

Available Human Cytokine and Chemokine Assays
Human Chemokine Analytes 40-plex Panel
(171-AK99MR2)
Singleplex Sets* Bead Region
6Ckine / CCL21 171-BK11MR2 12
BCA-1 / CXCL13 171-BK12MR2 74
CTACK / CCL27 171-BK13MR2 72
ENA-78 / CXCL5 171-BK14MR2 73
Eotaxin / CCL11 171-BK15MR2 43
Eotaxin-2 / CCL24 171-BK16MR2 30
Eotaxin-3 / CCL26 171-BK17MR2 65
Fractalkine / CX3CL1 171-BK18MR2 77
GCP-2 / CXCL6 171-BK19MR2 15
GM-CSF 171-BK21MR2 34
Gro-α / CXCL1 171-BK22MR2 61
Gro-β / CXCL2 171-BK23MR2 78
I-309 / CCL1 171-BK24MR2 20
IFN-γ 171-BK25MR2 21
IL-1β 171-BK26MR2 39
IL-2 171-BK27MR2 38
IL-4 171-BK28MR2 52
IL-6 171-BK29MR2 19
IL-8 / CXCL8 171-BK31MR2 54
IL-10 171-BK32MR2 56
IL-16 171-BK33MR2 27
IP-10 / CXCL10 171-BK34MR2 48
I-TAC / CXCL11 171-BK35MR2 25
MCP-1 / CCL2 171-BK36MR2 53
MCP-2 / CCL8 171-BK37MR2 57
MCP-3 / CCL7 171-BK38MR2 26
MCP-4 / CCL13 171-BK39MR2 28
MDC / CCL22 171-BK41MR2 29
MIF 171-BK42MR2 35
MIG / CXCL9 171-BK43MR2 14
MIP-1α / CCL3 171-BK44MR2 55
MIP-1δ / CCL15 171-BK46MR2 66
MIP-3α / CCL20 171-BK47MR2 62
MIP-3β / CCL19 171-BK48MR2 76
MPIF-1 / CCL23 171-BK49MR2 37
SCYB16 / CXCL16 171-BK51MR2 64
SDF-1α+β / CXCL12 171-BK52MR2 22
TARC / CCL17 171-BK53MR2 67
TECK / CCL25 171-BK54MR2 46
TNF-α 171-BK55MR2 36

Available Human Cytokine Assays

Human Group I
8-Plex Panel (M50-000007A)
17-Plex Panel (M50-00031YV)
27-Plex Panel (M50-0KCAF0Y)
Th1/Th2 Panel (M50-00005L3)
Singleplex/ x-Plex Bead Regions**
IL-1β
 
• 
• 
 
39
IL-1ra    
• 
 
25
IL-2
• 
• 
• 
• 
38
IL-4
• 
• 
• 
• 
52
IL-5  
• 
• 
• 
33
IL-6
• 
• 
• 
 
19
IL-7  
• 
• 
 
74
IL-8
• 
• 
• 
 
54
IL-9    
• 
 
77
IL-10
• 
• 
• 
• 
56
IL-12 p70  
• 
• 
• 
75
IL-13  
• 
• 
• 
51
IL-15    
• 
 
73
IL-17  
• 
• 
 
76
Basic FGF    
• 
 
44
Eotaxin    
• 
 
43
G-CSF  
• 
• 
 
57
GM-CSF
• 
• 
• 
• 
34
IFN-γ
• 
• 
• 
• 
21
IP-10    
• 
 
48
MCP-1 (MCAF)
 
• 
• 
 
53
MIP-1α    
• 
 
55
MIP-1β  
• 
• 
 
18
PDGF-BB    
• 
 
47
RANTES    
• 
 
37
TNF-α
• 
• 
• 
• 
36
VEGF    
• 
 
45

Human Group II
21-Plex Panel
(MF0-005KMII)
Singleplex/ x-Plex Bead Regions**
IL-1α
• 
63
IL-2Rα
• 
13
IL-3
• 
64
IL-12 (p40)
• 
28
IL-16
• 
27
IL-18***
• 
42
CTACK
• 
72
GRO-α
• 
61
HGF
• 
62
ICAM-1
 
12
IFN-α2
• 
20
LIF
• 
29
MCP-3
• 
26
M-CSF
• 
67
MIF
• 
35
MIG
• 
14
β-NGF
• 
46
SCF
• 
65
SCGF-β
• 
78
SDF-1α
• 
22
TNF-β
• 
30
TRAIL
• 
66
V-CAM1  
15

Th17 Assays
15-Plex Panel
(171-AA001M)
Singleplex
Bead Regions
IL-1β
• 
39
IL-4
• 
52
IL-6
• 
19
IL-10
• 
56
IL-17A
• 
76
IL-17F
• 
44
IL-21
• 
47
IL-22
• 
18
IL-23
• 
43
IL-25
• 
55
IL-31
• 
62
IL-33
• 
46
IFN-γ
• 
21
sCD40L
• 
29
TNF-α
• 
36
IL-17A/F  
66

TGF-β Group
3-Plex Panel
(171-W4001M)
Singleplex
Bead Regions
TGF-β1
• 
13
TGF-β2
• 
72
TGF-β3
• 
66

* Require reagent kit III (171-304090M or 171-304090) and standard (171-DK0001).

** Custom assays can be ordered with any combination of analytes within a group, except for ICAM-1 and VCAM-1, which require different sample dilutions. For more information about compatibility between groups see bulletin 5927.

*** IL-18 is not available as a singleplex assay, only as part of an assay panel.

† IL-17A/F is available only as a singleplex set and cannot be mixed with IL-17A or IL-17F.

‡ TGF-β assays are compatible with human, mouse, and rat samples.

Bio-Plex Assay Builder

To design your customized Express or x-Plex assay, process your catalog number, and obtain pricing information, launch the Assay Builder

To create a Bio-Plex custom assay, click Launch Bio-Plex Assay Builder.

Launch Bio-Plex Assay Builder



Bio-Plex® Technology and Applications

The Bio-Plex Pro™ Human Th17 Cytokine Panel is a unique blend of magnetic bead–based immunoassays designed to meet the needs of the most discerning preclinical and clinical researchers. The multiplex format enables robust and reproducible measurement of 15+1 soluble proteins involved in the T-helper cell type 17 immune response pathway, which is thought to play a key role in inflammatory conditions such as:

  • Rheumatoid arthritis, lupus, psoriasis, multiple sclerosis, and other autoimmune diseases
  • Antimicrobial immunity
  • Transplant rejection
  • Cancer

The panel enables measurement of the following analytes in diverse matrices such as serum, plasma, and cell culture supernatants:

  • IL-1β
  • IL-4
  • lL-6
  • IL-10
  • IL-17A
  • IL-17F
  • IL-17A/F (singleplex only)
  • IL-21
  • IL-22
  • IL-23
  • IL-25
  • IL-31
  • IL-33
  • IFN-γ
  • sCD40L
  • TNF-α
  • Based on 6.5 µm magnetic beads, these assays are compatible with both vacuum and magnetic wash stations. Magnetic washing simplifies plate processing, provides increased throughput, and results in decreased variability and increased reproducibility.

    Key Features

    • Reagent kit ll with diluents optimized for serum and plasma samples
    • Assay quick guide to get you started right away
    • Requires as little as 12.5 µl of sample for both multiplex and singleplex assays
    • Includes premixed standards for generation of standard curves over a broad dynamic range for each target
    • 2-level quality controls included with premixed kits

    Flexible Ordering Options

    Additional Th17 Pathway Assays:

    • TGF-β 3-plex panel (#171-V4003M)
    • TGF-β singleplex sets — each set includes a vial of coupled magnetic beads and a vial of detection antibodies; requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-D60001) for a complete assay

    More Information

    See how Bio-Plex Instruments and Assays benefit researchers studying heart disease, leukemia, melanoma, and other cancers.

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    Bio-Plex Pro™ Human Th17 Cytokine Panel 15-Plex

    171-AA001M
    1 x 96-well all-in-one kit, includes coupled magnetic beads, detection antibodies, standards, controls, buffers, diluents, streptavidin-PE, 96-well flat bottom plate, and sealing tape, for the detection of 15 human Th17 cytokines

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    Bio-Plex Pro™ Human Th17 Cytokine IL-1β Set

    171-BA001M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-1β, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine lL-4 Set

    171-BA002M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-4, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine lL-6 Set

    171-BA003M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human lL-6, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-10 Set

    171-BA004M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-10, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-17A Set

    171-BA005M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-17F Set

    171-BA006M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17F, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-21 Set

    171-BA007M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-21, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-22 Set

    171-BA008M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-22, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-23 Set

    171-BA009M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-23, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-25 Set

    171-BA010M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-25, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-31 Set

    171-BA011M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-31, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-33 Set

    171-BA012M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-33, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IFN-γ Set

    171-BA013M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IFN-γ, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine sCD40L Set

    171-BA014M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human sCD40L, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine TNF-α Set

    171-BA015M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human TNF-α, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ Human Th17 Cytokine IL-17A/F Set

    171-BA016M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting human IL-17A/F, requires reagent kit II (#171-304055 for vacuum separation or #171-304055M for magnetic separation) and a vial of standards (#171-DA0001)

    List Price:   $219.00
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    Bio-Plex Pro™ TGF-β1 Set

    171-V4001M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β1, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

    List Price:   $242.00
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    Bio-Plex Pro™ TGF-β2 Set

    171-V4002M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β2, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

    List Price:   $242.00
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    Bio-Plex Pro™ TGF-β3 Set

    171-V4003M
    1 x 96-well, includes coupled magnetic beads and detection antibodies for detecting TGF-β3, requires reagent kit (#171-304070 for vacuum separation or #171-304070M for magnetic separation) and a vial of standards (#171-X40001)

    List Price:   $242.00
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    Number Description Options
    6335 Bio-Plex® Multiplex Immunoassays Analyte Guide, Interactive PDF, Rev D Click to download [ Add to Cart (Free) ]
    6249 Bio-Plex Pro™ Human Th17 Cytokine Assays, Rev B Click to download [ Add to Cart (Free) ]
    6251 Multiplex Analysis of Inflammatory Markers Using Bio-Plex Pro Human Th17 Cytokine Assays, Rev A Click to download [ Add to Cart (Free) ]
    10022581 Instruction Manual, Bio-Plex Pro Assays Quick Guide, Rev C Click to download
    10023381 Instruction Manual, Bio-Plex Pro™ Human Th17 Cytokine Assays, Rev A Click to download
    6457 The Bio-Plex Success Guide for Multiplex Immunoassays, Rev B Click to download
    6458 Bio-Plex Success Guide/Assay Sample Prep, Rev B Click to download