Bio-Gel Hydroxyapatite HT and HTP Resin

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Bio-Gel HT and Bio-Gel HTP Resin

Overview

Specifications
  Bio-Gel HTP Gel Bio-Gel HT Gel, DNA Grade Bio-Gel HTP Gel
Flow rate* (cm/hr) >35 >5 >25
BSA adsorbed** (mg per dry gram) 10 10 10
Calf thymus DNA adsorbed (mg per dry gram) >500 >800 >500
Hydrated volume 2–3 ml/g 2–3 ml/g

* Flow rate determined on a 1.5 x 10 cm column with 40 cm hydrostatic pressure.
** Batchwise uptake.

Bio-Gel HT and HTP Media
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Hydroxyapatite (Ca5(PO4)3(OH)2 is a form of calcium phosphate that can be used for the separation or purification of proteins, enzymes, nucleic acids, viruses, and other macromolecules. Hydroxyapatite chromatography can be used at any stage, from initial capture to final polishing. Hydroxyapatite has unique selectivity and resolution, and often separates proteins that appear to be homogeneous by other chromatographic and electrophoretic techniques.

Bio-Gel crystalline hydroxyapatite is chemically and thermally stable, is compatible with a wide range of aqueous and organic solvents, and can be sanitized in NaOH. It has a pH tolerance of >5.5 and is autoclavable. Three types of resins are available:

Bio-Gel HT gel— Ideal for the purification of proteins, nucleic acids, and other macromolecules, Bio-Gel HT is prepared by the method of Tiselius (1956). It is suspended in 10 mMsodium phosphate buffer containing 0.02% NaN3 and should be stored at 4°C.

Bio-Gel HTP gel— This is the material described by Tiselius, which has been dried by a unique process developed by Bio-Rad. It may be stored without refrigeration.When suspended in buffer, it has characteristics similar to Bio-Gel HT gel; 1 g hydrates to 2–3 ml.

DNA grade Bio-Gel HTP gel— A dry powder with a smaller particle size than HTP gel, this gel significantly increases its capacity and enhances its selectivity for double-stranded DNA. Due to its small particle size, it is recommended for batch mode chromatography or for use with very short columns.

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Bio-Gel HT Hydroxyapatite

1300150
250 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer

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Bio-Gel HT Hydroxyapatite

1300151
500 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer

1 1300151 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/130-0151_0921120653507.xml 1300151 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:07:58 1300151-bio-gel-ht-hydroxyapatite 1300151 Bio-Gel HT Hydroxyapatite Bio-Gel HT Hydroxyapatite 500 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer 500 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer 500 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer /webroot/web/images/ps/products/multimodal_media/sku_thumb/global/130-0151_tn.jpg /webroot/web/images/ps/products/multimodal_media/sku_view/global/130-0151_view.jpg /webroot/web/images/ps/products/multimodal_media/sku_feature/global/130-0151_feature.jpg RepEx 130-0151 500 ml, hydrated hydroxyapatite gel suspended in sodium phosphate buffer 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300151&CartQuote=QT&quantity= false true false $795.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300151&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300151&quantity= 1300151
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Bio-Gel HTP Hydroxyapatite

1300420
100 g, hydroxyapatite chromatography media, powder, 1 g hydrates to 2–3 ml of gel

2 1300420 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/130-0420_0921120653574.xml 1300420 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:07:58 1300420-bio-gel-htp-hydroxyapatite 1300420 Bio-Gel HTP Hydroxyapatite Bio-Gel HTP Hydroxyapatite 100 g, hydroxyapatite chromatography media, powder, 1 g hydrates to 2–3 ml of gel 100 g, hydroxyapatite chromatography media, powder, 1 g hydrates to 2–3 ml of gel 100 g, hydroxyapatite chromatography media, powder, 1 g hydrates to 2–3 ml of gel /webroot/web/images/lsr/products/chromatography/sku_thumb/global/130-0420_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/130-0420_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/130-0420_feature.jpg RepEx 130-0420 100 g, hydroxyapatite chromatography media, powder, 1 g hydrates to 2–3 ml of gel 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300420&CartQuote=QT&quantity= false true false $500.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300420&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300420&quantity= 1300420
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Bio-Gel HTP Hydroxyapatite

130-0421
1 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel

3 1300421 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/130-0421_0921120653529.xml 1300421 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:07:58 1300421-bio-gel-htp-hydroxyapatite 1300421 Bio-Gel HTP Hydroxyapatite Bio-Gel HTP Hydroxyapatite 1 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel '1 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 1–3 ml of gel 1 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel /webroot/web/images/lsr/products/chromatography/sku_thumb/global/130-0421_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/130-0421_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/130-0421_feature.jpg RepEx 130-0421 '1 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 1–3 ml of gel 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300421&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300421&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300421&quantity= 130-0421
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Bio-Gel HTP Hydroxyapatite

130-0425
5 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel

4 1300425 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/130-0425_0921120653596.xml 1300425 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:07:58 1300425-bio-gel-htp-hydroxyapatite 1300425 Bio-Gel HTP Hydroxyapatite Bio-Gel HTP Hydroxyapatite 5 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel 5 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2—3 ml of gel 5 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2–3 ml of gel /webroot/web/images/lsr/products/chromatography/sku_thumb/global/130-0425_tn.jpg /webroot/web/images/lsr/products/chromatography/sku_view/global/130-0425_view.jpg /webroot/web/images/lsr/products/chromatography/sku_feature/global/130-0425_feature.jpg RepEx 130-0425 5 kg, hydroxyapatite HTP chromatography media, powder, 1 g hydrates to 2—3 ml of gel 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 / false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300425&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300425&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300425&quantity= 130-0425
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Bio-Gel HTP Hydroxyapatite

1300520
100 g, DNA grade hydroxyapatite chromatography media, powder, smaller particle size than HTP gel with enhanced selectivity for dsDNA

5 1300520 /templatedata/internet/product_SKU/data/LSR/Chromatography/Chromatography-Media/130-0520_0921120653640.xml 1300520 09/21/09 12:06 PM N en 09/21/19 12:06 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA LSR 2017-11-14 07:07:58 1300520-bio-gel-htp-hydroxyapatite 1300520 Bio-Gel HTP Hydroxyapatite Bio-Gel HTP Hydroxyapatite 100 g, DNA grade hydroxyapatite chromatography media, powder, smaller particle size than HTP gel with enhanced selectivity for dsDNA 100 g, DNA grade hydroxyapatite chromatography media, powder, smaller particle size than HTP gel with enhanced selectivity for dsDNA 100 g, DNA grade hydroxyapatite chromatography media, powder, smaller particle size than HTP gel with enhanced selectivity for dsDNA /webroot/web/images/ps/products/multimodal_media/sku_thumb/global/130-0520_tn.jpg /webroot/web/images/ps/products/multimodal_media/sku_view/global/130-0520_view.jpg /webroot/web/images/ps/products/multimodal_media/sku_feature/global/130-0520_feature.jpg RepEx 130-0520 100 g, DNA grade hydroxyapatite chromatography media, powder, smaller particle size than HTP gel with enhanced selectivity for dsDNA 09/21/09 12:06 PM 09/21/19 12:06 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en LSR /LSR/Chromatography/Chromatography-Media N 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300520&CartQuote=QT&quantity= false true false $900.00 false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300520&AddToDefaultWishList=true&quantity= true false true true false true true true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1300520&quantity= 1300520
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(Discontinued) Econo-Column Open-Ended Jacketed Chromatography Column

7376201
Pkg of 1, 1 x 30 cm, chromatography column, max. vol. 25 ml, cross-sectional area 0.79 cm2, includes 2 flow adaptors for DNA hydroxyapatite chromatography

This product is no longer available. Please contact technical support for more information on replacement product.
Number Description Options
LIT217 Instruction Manual, Bio-Gel HT, Bio-Gel HTP, DNA Grade Bio-Gel HTP, Hydroxyapatite, Rev C Click to download

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