Benzodiazepines and Tricyclics by HPLC

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Benzodiazepines and Tricyclics by HPLC

Bio-Rad's Benzodiazepines and Tricyclics Reagent Kit provides a reliable, fast and easy-to-use method for the analysis of 25 antidepressants, benzodiazepines, selective serotonin re-uptake inhibitors (SSRIs), and their pharmacologically active metabolites. The analytes are extracted and separated with an HPLC method that involves online column switching between an extraction cartridge and an analytical column. This application is compatible with Bio-Rad's RAPsyD by HPLC assay, as both tests use the same analytical columns, extraction cartridges and mobile phases. With its fully automated HPLC method, this assay allows for online sample preparation and does not require manual pipetting steps, making it a time-saving and convenient method. Benzodiazepines & Tricyclic Antidepressants by HPLC gives physicians an efficient tool for initial determination of dosage and for subsequent therapeutic drug monitoring.

Test characteristics:

  • Principle: Isocratic HPLC method (Reversed Phase)
  • Duration of the chromatography: 35 min. (Analyte panel A) / 25 min. (Analyte panels B–E)
  • Sample volume: 100 µL serum or plasma
  • Flow rate: 1.1 mL/min (Analyte panel A) / 0.6 mL/min (Analyte panels B–E),
  • UV Detection: 233 nm (Analyte panels A, C & E) / 250 nm (Analyte panels B & D)

 

Benzo/TCA by HPLC Reagent Kit
Benzo/TCA by HPLC Reagent Kit

195-6550
Complete reagent kit for the analysis of 25 antidepressants, benzodiazepines, and SSRIs by HPLC (reversed phase), including Standard Extraction Set, TDM Mobile Phase 1 Set and TDM Mobile Phase 2 Set (200 tests)

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Standard Extraction Set
Standard Extraction Set

195-6556
Contains internal standards, Calibrators A, B, C, and D, and extraction cartridge for Benzodiazepines and Tricyclics by HPLC assay (1 set)

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Analytical Column for TDM by HPLC
Analytical Column for TDM by HPLC

195-6684
Analytical column and microguard cartridge for therapeutic drug monitoring by Benzodiazepines and Tricyclics and RAPsyD HPLC assays (600/2 x 250 injections)

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Holder for Extraction Cartridge
Holder for Extraction Cartridge

270-0613
Holder for extraction cartridge used to prepare samples for online reverse-phase HPLC assays (1 x 1)

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Prefilter Holder, Online Method
Prefilter Holder, Online Method

195-6682
Prefilter holder for HPLC assay; online method (1 unit)

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Micro-Guard Cartridge Holder

195-5030
1 holder for high performance liquid chromatography guard cartridge

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Calibrator A (mono-level) for Benzo/TCA by HPLC Reagent Kit
Calibrator A (mono-level) for Benzo/TCA by HPLC Reagent Kit

195-6552
Calibrator set for 10 trycyclic antidepressants; for use with Benzodiazepines and Tricyclics by HPLC (1 x 5 mL)

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Calibrator B (mono-level) for Benzo/TCA by HPLC Reagent Kit
Calibrator B (mono-level) for Benzo/TCA by HPLC Reagent Kit

195-6553
Calibrator set for 7 benzodiazepines; for use with Benzodiazepines and Tricyclics by HPLC (1 x 5 mL)

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Calibrator C (mono-level) for Benzo/TCA by HPLC Reagent Kit
Calibrator C (mono-level) for Benzo/TCA by HPLC Reagent Kit

195-6554
Calibrator set for nitrazipam, clonazepam and flunitrazepam; for use with Benzodiazepines and Tricyclics by HPLC (1 x 5 mL)

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Calibrator D (mono-level) for Benzo/TCA by HPLC Reagent Kit
Calibrator D (mono-level) for Benzo/TCA by HPLC Reagent Kit

195-6555
Calibrator set for bromazepam, lorazepam, and temazepam;for use with Benzodiazepines and Tricyclics by HPLC (1 x 5 mL)

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Calibrator E (mono-level) for Benzo/TCA by HPLC Reagent Kit
Calibrator E (mono-level) for Benzo/TCA by HPLC Reagent Kit

195-6569
Calibrator set for norclobazam and clobazam, for use with Benzodiazepines and Tricyclics by HPLC (1 x 5 mL)

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Control Set A (bi-level) for Benzo/TCA by HPLC Reagent Kit
Control Set A (bi-level) for Benzo/TCA by HPLC Reagent Kit

195-6559
Bi-level control set for 10 trycyclic antidepressants; for use with Benzodiazepines and Tricyclics by HPLC (2 x 2 x 5 mL)

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Control Set B (bi-level) for Benzo/TCA by HPLC Reagent Kit
Control Set B (bi-level) for Benzo/TCA by HPLC Reagent Kit

195-6562
Bi-level control set for 7 benzodiazepines; for use with Benzodiazepines and Tricyclics by HPLC (2 x 2 x 5 mL)

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Control Set C (bi-level) for Benzo/TCA by HPLC Reagent Kit
Control Set C (bi-level) for Benzo/TCA by HPLC Reagent Kit

195-6565
Bi-level control set for clonazepam and flunitrazepam; for use with Benzodiazepines and Tricyclics by HPLC (2 x 2 x 5 mL)

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Control Set D (bi-level) for Benzo/TCA by HPLC Reagent Kit
Control Set D (bi-level) for Benzo/TCA by HPLC Reagent Kit

195-6568
Bi-level control set for bromazepam, lorazepam, and temazepam;for use with Benzodiazepines and Tricyclics by HPLC (2 x 2 x 5 mL)

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Control Set E (bi-level) for Benzo/TCA by HPLC Reagent Kit
Control Set E (bi-level) for Benzo/TCA by HPLC Reagent Kit

195-6587
Bi-level control set for norclobazam and clobazam, for use with Benzodiazepines and Tricyclics by HPLC (2 x 2 x 5 mL)

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TDM by HPLC Internal Standard 1
TDM by HPLC Internal Standard 1

195-6626
Internal Standard 1 for use with TDM by HPLC assays (1 x 4 mL)

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TDM by HPLC Internal Standard 2
TDM by HPLC Internal Standard 2

195-6551
Internal Standard 2 for use with TDM by HPLC assays (1 x 4 mL)

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TDM by HPLC Mobile Phase 1 Set
TDM by HPLC Mobile Phase 1 Set

195-6686
Mobile phase 1 for therapeutic drug monitoring by Benzodiazepines and Tricyclics and RAPsyD HPLC (4 x 1 L)

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TDM by HPLC Mobile Phase 2 Set
TDM by HPLC Mobile Phase 2 Set

195-6687
Mobile phase 2 for therapeutic drug monitoring by Benzodiazepines and Tricyclics and RAPsyD HPLC (4 x 1 L)

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Micro-Guard Refill Cartridges

195-6003
Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections

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HPLC Prefilter
HPLC Prefilter

195-6636
Two replacement prefilters for HPLC; 250 analyses each (1 package)

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Extraction Cartridges (Includes 2 Prefilters)
Extraction Cartridges (Includes 2 Prefilters)

195-6685
Extraction cartridge for therapeutic drug monitoring by Benzodiazepines and Tricyclics and RAPsyD HPLC assays (1 x 100 injections)

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