Aurum™ Total RNA Fatty and Fibrous Tissue Kit

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Overview

RNA Isolation Selection Guide
  Aurum Total RNA Kits PureZOL RNA Isolation Reagent
Mini Fatty and Fibrous Tissue 96
Format Mini column Filtration (vacuum or spin) Mini column Filtration (vacuum or spin) 96-well plate Filtration (vacuum or spin)
Maximum starting amounts        
   Cultured cells 2 x 106 1 x 106 1 x 107
   Bacterial cells 2.4 x 109 8 x 108 2.4 x 109
   Yeast cells 3 x 107 2 x 107 3 x 107
   Animal tissue — hard 20 mg 100 mg 100 mg
   Animal tissue — soft    to moderately hard 40 mg 100 mg 100 mg
   Plant tissue 40 mg 100 mg 100 mg
Isolation method Silica membrane Lysis with PureZOL reagent, purification on silica membrane Silica membrane Organic extraction
Number of preps 10* or 50 mini preps 10* or 50 mini preps 2 x 96-well plate 50 or 100 preps (1 ml/prep)
Number of washes 3 3 3
DNase I included** Yes Yes Yes No
DNase I digest time 15 min (animal tissue, 25 min) 15 min 10 min
Total preparation time <50min (1.5 hr with DNase I) <50min (1.5 hr with DNase I) <60 min <60 min
Binding capacity >100 µg >100 µg >40 µg
Elution volume 2 x 40 µl 2 x 40 µl 80 µl 30–100 µl
Yield*** 28 µg/2 x 106 NIH 3T3 cells 96 µg/100 mg brain 10 µg/1 x 106 NIH 3T3 cells 1.15 µg/mg brain
  38 µg/2 x 106 HeLa cells 58 µg/100 mg breast 17 µg/1 x 106 HeLa cells 1.37 µg/mg fat
  20 µg/40 mg brain 85 µg/100 mg heart 11 µg/2 x 107 S. cerevisiae cells 1.2 µg/mg heart
  52 µg/20 mg thymus 63 µg/100 mg skin 5 µg/8 x 108 E. coli cells 0.66 µg/mg cartilage
  132 µg/40 mg spleen 54 µg/100 mg cartilage 5 µg/8 x 108 B. cereus cells 1.2 µg/mg skin
  36 µg/40 mg lung     1.0 µg/mg potato
  32 µg/40 mg potato     1.37 µg/1 x 105 293H cells
  28 µg/3 x 107 S. cerevisiae cells     0.1 µg/mg Arabidopsis
  54 µg/2.4 x 109 E. coli cells     0.38 µg/mg A. niger
        0.63 µg/2.4 x 107 E. coli cells
        0.31 µg/3 x 105 S. cerevisiae cells
Purity A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1 A260/A280 of 1.9–2.1
 

* Trial sizes of the Aurum total RNA mini kit and Aurum total RNA fatty and fibrous tissue kit are available.

** Removal not required.

*** Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.

Isolated RNA Quality

Analysis of high-quality RNA isolated with the Aurum total RNA fatty and fibrous tissue kit. Electropherogram of high-quality mouse brain total RNA analyzed on the Experion automated electrophoresis system using the Experion RNA StdSens kit (left) and denaturing agarose gel of RNA samples (4 µg) from mouse brain and skeletal muscle (right).

Isolated RNA Performance via qPCR

Excellent performance of RNA isolated by the Aurum total RNA fatty and fibrous tissue kit in real-time RT-PCR. RNA was isolated from 50 mg of mouse brain (upper panel) and skeletal muscle (lower panel), then reverse transcribed using the iScript cDNA synthesis kit. cDNA was amplified using iQ SYBR® Green supermix and β-actin primers and analyzed with the MyiQ single-color real-time PCR detection system.

The Aurum total RNA fatty and fibrous tissue kit produces high yields of exceptionally pure total RNA from samples that are difficult to disrupt. The kit is perfect for purifications involving fatty and fibrous tissues or samples such as fungi that are rich in RNases. It also works well with most animal and plant tissues, cultured cells, yeast, and bacteria.

The Aurum total RNA fatty and fibrous tissue kit combines the best features of PureZOL™ RNA reagent, a potent phenol-based reagent that effectively lyses tissues and cells, with the speed of silica membrane technology in a spin-column format to yield high-quality, intact RNA in minimal time.

The entire purification process, including DNase I digestion, is completed in less than 60 minutes. The kit includes mini spin columns that can be processed using a choice of vacuum- or spin-mediated protocols. Isolated RNA is of the highest integrity and is suitable for the most discriminating applications.

Features include:

  • High-quality RNA isolation from difficult-to-process samples in less than 60 minutes
  • RNase-free reagents and plasticware; includes DNase I for the removal of genomic DNA
  • Rapid silica binding-based purification technology in a spin-column format
  • Easy-to-use spin- or vacuum-compatible protocol
Format Mini column
Method Spin- or vacuum-mediated silica binding
Number of preps 50
Maximum starting material amounts 100 mg animal or plant tissues
  50 mg filamentous fungi
  1 x 107 cultured cells
  2.4 x 109 bacterial cells
  3 x 107 yeast cells
Binding capacity >100 µg purified total RNA
Purity A260/A280 of 1.9–2.1
RNA size distribution >200 nucleotides
Preparation time 30–60 min (including DNase I digest)
Maximum elution volume 80 µl (2 elutions of 40 µl each)
Minimum elution volume 30 µl*
Yield** 96 µg/100 mg brain
  58 µg/100 mg breast
  85 µg/100 mg heart
  63 µg/100 mg skin
  54 µg/100 mg cartilage
  91 µg/100 mg plant tissue
  12 µg/50 mg filamentous fungi
  145 µg/1 x 107 293-H cultured cells
  30 µg/2.4 x 109 E. coli
  64 µg/3 x 107 S. cerevisiae
*The elution volume is reduced to 30µl
when isolating total RNA from small amounts of
starting material (<500,000 cells or 10 mg of tissue).
 
**Yields will vary depending on the development stage, tissue type or cell line, and growth conditions used.  
Aurum™ Total RNA Fatty and Fibrous Tissue Kit

732-6830
DNA-free RNA isolation kit, includes PureZOL RNA isolation reagent, RNA binding columns, all collection and sample tubes, lyophilized DNase I, RNase-free reagents and plasticware, for 50 preps

List Price:   $427.00
Quantity:   Add to cart
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Aurum™ Total RNA Fatty and Fibrous Tissue Module

732-6870
DNA-free RNA isolation kit, includes columns and tubes, DNase I, RNase-free reagents (PureZOL RNA isolation reagent sold separately), for 50 preps

List Price:   $294.00
Quantity:   Add to cart
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Number Description Options
6090 Amplification Reagents and Plastics Brochure, Rev D Click to download [ Add to Cart (Free) ]
5282 Aurum Total RNA Fatty and Fibrous Tissue Kit Flier, Rev A Click to download
5257 Aurum Total RNA Fatty and Fibrous Tissue Kit Protocol Overview, Rev A Click to download
10001298 Instruction Manual, Aurum Total RNA Fatty and Fibrous Tissue Kit, Rev B Click to download
5990 Gene Expression Analysis Solutions Brochure, Rev C Click to download [ Add to Cart (Free) ]