Bio-Rad’s Mueller-Hinton Agar is manufactured according to WHO standards, and tested according to the acceptance limits described in C.L.S.I. and EUCAST documents.
It is produced to contain low levels of thymine and thymidine (which inhibit sulfonamide and trimethoprim activity), and controlled levels of calcium and magnesium to obtain appropriate activity of aminoglycosides.
Bio-Rad Mueller-Hinton-F agar medium (Mueller-Hinton + 5% horse blood + ß-NAD, ß-Nicotinamide Adenine Dinucleotide) is used for antimicrobial susceptibility testing of Streptococcus spp., Haemophilus spp., in accordance with EUCAST guidelines.
The pH is 7.4 ± 0.2, and the agar depth in the Petri dish is uniform to approximately 4 mm.
CLSI: Clinical and Laboratory Standard Institute
EUCAST: European Committee on Antimicrobial Susceptibility Testing