Affi-Prep Protein A Media

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Overview

Capacities of Protein A Supports
Immunoglobulin Affi-Gel® Protein A
Capacity (mg/ml)
Affi-Prep® Protein A
Capacity (mg/ml)
Mouse lgG1 6–10 7–9
Mouse lgG2a 6–10 7–9
Mouse lgG2b 6–10 7–9
Mouse lgG3 6–10 7–9
Mouse lgM* 6–10 7–9
Human lgG 15 10–12
Sheep lgG 8–10 7–9
Cow lgG 8–10 7–9
Horse lgG 8–10 7–9
Goat lgG 8–10 7–9
Rabbit lgG 8–10 7–9
Dog lgG 8–10 7–9
Pig lgG 8–10 7–9

* Approximately 50% of all mouse lgMs bind using the MAPS buffer system.

Binding Capacity Chart

Binding capacity for murine IgG subclasses. Mouse ascites fluids, containing equivalent amounts (5 mg) of either mouse IgG1, IgG2a, or IgG2b monoclonal antibody, were purified with 0.5 ml columns of Affi-Prep protein A matrix. Chromatography was performed with the Affi-Prep protein A MAPS II buffers.

Chromatography on Affi-Prep protein A media yields highly purified immunoglobulins (IgG), selectively removes IgG prior to analysis of other immunoglobulin classes, or adsorbs immune complexes for antigen purification. Protein A binds to the Fc region of immunoglobulins, especially IgGs from mammalian species.

The Affi-Prep protein A matrix allows high linear flow rates and exhibits excellent mechanical strength. These key features allow the direct transfer of experimental conditions from the initial analytical methods development stage to the final production scale purification of product. When the Affi-Prep protein A matrix is used in conjunction with the optimized Affi-Prep protein A MAPS II buffers, high efficiency binding of a wide range of immunoglobulins, including all isotypes of murine IgG, is achieved.

Advantages of the Affi-Prep protein A support include:

  • Binds a wide range of monoclonal and polyclonal antibodies, including mouse IgG1
  • Macroporous polymeric support is not compressible under normal operating conditions
  • Linear flow rates up to 2,000 cm/hr are achievable
  • High flow rates at all column diameters for easy scale-up
  • Compatible with most commonly used chromatographic buffers
  • Stable pH range from 2–10
  • Multiple chromatographic cycles without loss of capacity
  • High purity of IgGs
  • High affinity for mammalian IgG
  • High capacities for mouse IgG1 as well as other subclasses with MAPS optimized buffer
  • Easy to use
Affi-Prep Protein A Support

156-0006
5 ml, ready-to-use pressure-stable polymer support

List Price:   $498.00
Quantity:   Add to cart
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Affi-Prep Protein A Support

156-0005
25 ml, ready-to-use pressure-stable polymer support

List Price:   $2,366.00
Quantity:   Add to cart
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Number Description Options
5584 Bio-Scale Mini Cartridges, Product Information Sheet, Rev B Click to download
LIT230 Instruction Manual, Affi-Prep Protein A Matrix Click to download [ Add to Cart (Free) ]
LIT79 Instruction Manual, Affi-Prep Protein A MAPS II Kit Click to download [ Add to Cart (Free) ]
5712 The Profinia Protein Purification System Simplifies Antibody Purification With Protein A, Rev A Click to download [ Add to Cart (Free) ]
5726 Profinia Protein A and G methods Product Information Sheet, Rev A Click to download [ Add to Cart (Free) ]
5701 Profinia Buffers for Protein A and Protein G Chromatography PIS, Rev B Click to download [ Add to Cart (Free) ]