Affi-Gel boronate-derivatized polyacrylamide gel has affinity for coplanar adjacent cis-hydroxyl groups (cis-diols) and a high binding capacity, which provides highly efficient separation of low molecular weight molecules, such as nucleotides, nucleosides, catecholamines, and sugars. It has a sorbitol capacity of 130 µmol/ml.
Other features include:
- High boronate capacity — 1.05 ± 0.15 meq/g
- High diol capacity — binds 130 µmol sorbitol/ml gel
- Spherical polyacrylamide matrix — excellent flow rate, resolution, and reproducibility
- Low nonspecific absorption, optimum porosity — low exclusion limit precludes significant binding of high molecular weight molecules, while allowing rapid equilibration and binding of low and medium molecular weight molecules