25-OH Vitamin D3/D2 by HPLC (Reversed Phase)

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25-OH Vitamin D3/D2 by HPLC (Reversed Phase)


The Bio-Rad 25-OH Vitamin D3/D2 by HPLC assay provides precise results for circulating 25-OH Vitamin D3 and 25-OH Vitamin D2 levels. This improved application can be used for simultaneous measurement of the discrete values of 25-OH Vitamin D3 and 25-OH Vitamin D2. The HPLC method allows for precise monitoring of response, useful for daily routine analysis to therapy in patients.

Bio-Rad provides customers with a human serum based calibrator, containing defined Vitamin D3 and D2 values traceable to the Vitamin D Standard Reference Material® 972 from NIST (The National Institute of Standards and Technologies, an agency of the U.S. Department of Commerce).

Vitamin D is an essential bio-regulator of calcium metabolism and thus significant for bone mineralization. Vitamin D deficiency is a recognized cause of secondary hyperparathyroidism, increased bone turnover and bone loss (ostepenia) in eldery people. Therefore, Vitamin D testing is essential in the management of patients with various disorders of calcium metabolism.

Recent studies have suggested that Vitamin D may play a role in protecting against cancer and in regulating normal immune function. Deficiency has been linked with autoimmune conditions such as type 1 diabetes, multiple sclerosis and rheumatoid arthritis. Vitamin D supplementation to prevent and treat deficiency can be provided in the form of 25-OH Vitamin D3 or 25-OH Vitamin D2. Serum concentration of 25-OH Vitamin D has been accepted as the clinical indicator of a patient's Vitamin D status. In order to identify and correct the deficiency of these 25-OH metabolites, an accurate measurement is essential.

Test characteristics:

  • Principle: Isocratic HPLC method (Reversed Phase)
  • Flow rate: 1.1 mL/min
  • Duration of chromatography: 12 minutes
  • UV Detection: 265 nm
  • Sample volume: 500 µL serum or plasma

 

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Micro-Guard Cartridge Holder

195-5030
1 holder for high performance liquid chromatography guard cartridge

7 1955030 /templatedata/internet/product_SKU/data/CDG/Special-Chemistry/Homocysteine-Assays-for-Risk-A/195-5030_0921120828381.xml 1955030 09/21/09 12:08 PM N en 09/21/19 12:08 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA CDG 2017-11-14 07:06:58 1955030-micro-guard-cartridge-holder 1955030 Micro-Guard Cartridge Holder Micro-Guard Cartridge Holder 1 holder for high performance liquid chromatography guard cartridge 1 holder for high performance liquid chromatography guard cartridge 1 holder for high performance liquid chromatography guard cartridge /webroot/web/images/cdg/products/special_chemistry/sku_thumb/global/scbu_195_5030_tn.jpg /webroot/web/images/cdg/products/special_chemistry/sku_view/global/scbu_195_5030_view.jpg /webroot/web/images/cdg/products/special_chemistry/sku_feature/global/scbu_195_5030_feature.jpg preview RepEx 195-5030 1 holder for high performance liquid chromatography guard cartridge 09/21/09 12:08 PM 09/21/19 12:08 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en CDG /CDG/Special-Chemistry/Homocysteine-Assays-for-Risk-A N 0 <p><strong><span class="hidden CA-only" style="color: #ff0000;">Unlicensed for Sale in Canada<br /> Non-homologu&eacute; pour la vente au Canada.</span></strong></p> /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global scbu_195-5030_sku_desc.html /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global/scbu_195-5030_sku_desc.html false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1955030&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1955030&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1955030&quantity= 195-5030
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Capillary Connection Set

195-4088
Set of hardware to connect 2 HPLC cartridges (1 unit)

8 1954088 /templatedata/internet/product_SKU/data/CDG/Special-Chemistry/Clinical-HPLC/195-4088_en_1320569831_1325.xml 1954088 07/29/11 5:00 PM N en 07/29/21 5:00 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA CDG 2017-11-14 07:06:57 1954088-capillary-connection-set 1954088 Capillary Connection Set Capillary Connection Set Set of hardware to connect 2 HPLC cartridges (1 unit) /webroot/web/images/cdg/products/special_chemistry/sku_thumb/global/scbu_195_4129_tn.jpg /webroot/web/images/cdg/products/special_chemistry/sku_view/global/scbu_195_4129_view.jpg /webroot/web/images/cdg/products/special_chemistry/sku_feature/global/scbu_195_4129_feature.jpg Capillary Connection Set Alex Samodurov Capillary Connection Set Set of hardware to connect 2 HPLC cartridges (1 unit) 195-4088, special chemistry, clinical hplc, biogenic amines, urinary catecholamines by hplc, reversed phase, capillary connection set 07/29/11 5:00 PM 07/29/21 5:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en CDG /CDG/Special-Chemistry/Clinical-HPLC/ N 0 <p><strong><span class="hidden CA-only" style="color: #ff0000;">Unlicensed for Sale in Canada<br /> Non-homologu&eacute; pour la vente au Canada.</span></strong></p> /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global scbu_195-4088_sku_desc.html /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global/scbu_195-4088_sku_desc.html 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1954088&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1954088&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1954088&quantity= 195-4088
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Serum Calibrator Set (mono-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit

195-6527
Serum calibrator set containing 25-OH vitamins D3 and D2, plus vitamin A, for HPLC assay (3 x 3 mL); traceable to the Vitamin D Standard Reference Material® 972 from NIST (The National Institute of Standards and Technologies, an agency of the U.S. Department of Commerce).

10 1956527 /templatedata/internet/product_SKU/data/CDG/Special-Chemistry/Clinical-HPLC/195-6527_en_1320569831_1371.xml 1956527 07/29/11 5:00 PM N en 07/29/21 5:00 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA CDG 2017-11-14 07:06:58 1956527-serum-calibrator-set-mono-level-for-25-oh-vitamin-d3-d2-hplc-reagent-kit 1956527 Serum Calibrator Set (mono-level) for 25-OH-Vitamin D3|D2 by HPLC Reagent Kit Serum Calibrator Set (mono-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit Serum calibrator set containing 25-OH vitamins D3 and D2, plus vitamin A, for HPLC assay (3 x 3 mL); traceable to the Vitamin D Standard Reference Material<sup>&reg;</sup> 972 from NIST (The National Institute of Standards and Technologies, an agency of the U.S. Department of Commerce). /webroot/web/images/cdg/products/special_chemistry/sku_thumb/global/scbu_195_6529_tn.jpg /webroot/web/images/cdg/products/special_chemistry/sku_view/global/scbu_195_6529_view.jpg Serum Calibrator Set (mono-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit Alex Samodurov Serum Calibrator Set (mono-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit Serum calibrator set containing 25-OH vitamins D3 and D2, plus vitamin A, for HPLC assay (3 x 3 mL); traceable to the Vitamin D Standard Reference Material<sup>&reg;</sup> 972 from NIST (The National Institute of Standards and Technologies, an agency of the U.S. Department of Commerce). 195-6527, special chemistry, clinical hplc, vitamin assays, vitamin assays - 25-oh-vitamin d3, serum calibrator set, mono-level for 25-oh-vitamin d3/d2 by hplc reagent kit 07/29/11 5:00 PM 07/29/21 5:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en CDG /CDG/Special-Chemistry/Clinical-HPLC/ N 0 <p><strong><span class="hidden CA-only" style="color: #ff0000;">Unlicensed for Sale in Canada<br /> Non-homologu&eacute; pour la vente au Canada.</span></strong></p> /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global scbu_195-6527_sku_desc.html /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global/scbu_195-6527_sku_desc.html 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956527&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956527&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956527&quantity= 195-6527
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Serum Control Set (bi-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit

195-6548
Bi-level serum control set for use with 25-OH Vitamin D3/D2 by HPLC assay (2 x 2 x 3 mL)

11 1956548 /templatedata/internet/product_SKU/data/CDG/Special-Chemistry/Clinical-HPLC/195-6548_en_1320569831_1377.xml 1956548 07/29/11 5:00 PM N en 07/29/21 5:00 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA CDG 2017-11-14 07:06:58 1956548-serum-control-set-bi-level-for-25-oh-vitamin-d3-d2-hplc-reagent-kit 1956548 Serum Control Set (bi-level) for 25-OH-Vitamin D3|D2 by HPLC Reagent Kit Serum Control Set (bi-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit Bi-level serum control set for use with 25-OH Vitamin D3/D2 by HPLC assay (2 x 2 x 3 mL) /webroot/web/images/cdg/products/special_chemistry/sku_thumb/global/scbu_195_6529_tn.jpg /webroot/web/images/cdg/products/special_chemistry/sku_view/global/scbu_195_6529_view.jpg Serum Control Set (bi-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit Alex Samodurov Serum Control Set (bi-level) for 25-OH-Vitamin D3/D2 by HPLC Reagent Kit Bi-level serum control set for use with 25-OH Vitamin D3/D2 by HPLC assay (2 x 2 x 3 mL) 195-6548, special chemistry, clinical hplc, vitamin assays, vitamin assays - 25-oh-vitamin d3, serum control set, bi-level for 25-oh-vitamin d3/d2 by hplc reagent kit 07/29/11 5:00 PM 07/29/21 5:00 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en CDG /CDG/Special-Chemistry/Clinical-HPLC/ N 0 <p><strong><span class="hidden CA-only" style="color: #ff0000;">Unlicensed for Sale in Canada<br /> Non-homologu&eacute; pour la vente au Canada.</span></strong></p> /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global scbu_195-6548_sku_desc.html /webroot/web/html/cdg/products/special_chemistry/sku_view/description/global/scbu_195-6548_sku_desc.html 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956548&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956548&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956548&quantity= 195-6548
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Micro-Guard Refill Cartridges

195-6003
Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections

12 1956003 /templatedata/internet/product_SKU/data/CDG/Special-Chemistry/Homocysteine-Assays-for-Risk-A/195-6003_0921120828269.xml 1956003 09/21/09 12:08 PM N en 09/21/19 12:08 PM true true internet/product_SKU AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA CDG 2017-11-14 07:06:58 1956003-micro-guard-refill-cartridges 1956003 Micro-Guard Refill Cartridges Micro-Guard Refill Cartridges Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections Pkg. of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections /webroot/web/images/cdg/products/special_chemistry/sku_thumb/global/scbu_195_6003_tn.jpg /webroot/web/images/cdg/products/special_chemistry/sku_view/global/scbu_195_6003_view.jpg /webroot/web/images/cdg/products/special_chemistry/sku_feature/global/scbu_195_6003_feature.jpg RepEx 195-6003 Package of 2 guard cartridges, each protects high performance liquid chromatography column for 250 injections 09/21/09 12:08 PM 09/21/19 12:08 PM AE,AI,AL,AM,AR,AT,AU,AZ,BA,BD,BE,BF,BG,BH,BN,BO,BR,BW,CA,CH,CL,CM,CN,CO,CR,CY,CZ,DE,DK,DO,DZ,EC,EE,EG,EH,ER,ES,ET,FI,FM,FO,FR,GA,GE,GF,GH,GP,GR,GT,GU,HK,HN,HR,HT,HU,ID,IE,IL,IN,IS,IT,JM,JO,JP,KE,KH,KR,KW,KZ,LB,LI,LK,LT,LU,LV,MA,MD,MG,MK,ML,MO,MQ,MS,MT,MU,MX,MY,NG,NI,NL,NO,NP,NZ,OM,PA,PE,PF,PG,PH,PK,PL,PR,PS,PT,PW,PY,QA,RO,RS,RU,SA,SB,SE,SG,SI,SK,SN,ST,SV,TG,TH,TN,TO,TR,TT,TW,TZ,UA,UG,UK,US,UY,UZ,VA,VE,VU,XK,YE,ZA en CDG /CDG/Special-Chemistry/Homocysteine-Assays-for-Risk-A N 0 false false false /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956003&CartQuote=QT&quantity= false true false null false null /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956003&AddToDefaultWishList=true&quantity= false false true true false true false true /prd/en/CA/adirect/biorad?cmd=ajaxAddToCart&AddToCartProductID=1956003&quantity= 195-6003
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