2-D Electrophoresis Workflow

Print
2-D Electrophoresis Workflow

Overview

2-D Electrophoresis Workflow How-To Guide

2-D Electrophoresis Workflow How-To Guide

Guides You Through 2-D Electrophoresis From Start to Finish

This manual provides in-depth information about:

  • Experimental methods and tools
  • Standard protocols
  • Ideas for developing customized protocols
  • Tips and tricks to improve results and reproducibility

Download PDF

2-D Doctor

Troubleshooting 2-D Electrophoresis Gels with 2-D Doctor™

The 2-D Doctor is a self-help guide developed by Bio-Rad that enables you to identify and troubleshoot your 2-D gel issues. Comprehensive solutions and suggestions are provided to help solve 2-D gel electrophoresis challenges.

Learn more

PROTEAN® i12™ IEF System Tour

12 Lanes. Individual Control. Total Confidence.

 

Simultaneously run 12 lanes, each with different samples, pH gradients, and protocols

  • Optimize experiments in fewer runs
  • Run multiple samples at once
  • Get more reproducible results

Learn how to:

  • Assemble trays, electrodes, and
    load samples
  • Use the easy touch-screen control to program and operate the system
  • Use the free web-based application for data analysis and protocol design

Launch the System Tour

2-D Video Tutorial

Bio-Rad's 2-D Electrophoresis Workflow provides a comprehensive selection of product solutions and educational resources that guide you to better 2-D results.

5 Steps

  1. Sample Preparation — sample preparation is the first step toward optimizing your results. Bio-Rad offers easy-to-use kits: ReadyPrep™ 2-D starter kit, ReadyPrep 2-D cleanup kit, and ProteoMiner™ protein enrichment kit for getting started and for cleanup and enrichment of low-abundance proteins.
  2. First Dimension Electrophoresis — the first dimension of 2-D electrophoresis is separation based on a protein's isoelectric point. Bio-Rad's IPG strips offer many pH gradient options and strict quality control. The award-winning PROTEAN® i12™ IEF system gives you the flexibility to run multiple pH gradients, protocols, and samples simultaneously.
  3. Second Dimension Electrophoresis — the second dimension of 2-D electrophoresis is separation based on molecular weight. Bio-Rad's Criterion™ system, Criterion precast gels, Criterion™ Dodeca™ cell, and Precision Plus Protein™ standard plugs provide the confidence of a leakproof precast gel and the ability to run up to 12 gels at once.
  4. Staining, Imaging, and Analysis — staining, imaging, and analysis allow you to document and evaluate your 2-D results. With Bio-Rad's sensitive and hassle-free Oriole™ gel stain and versatile ChemiDoc™ MP imager, you can obtain high-quality images. Analyze those images with PDQuest™ software, a highly powerful tool for 2-D evaluation.
  5. Spot Cutting — to analyze protein spots from your 2-D gel by mass spectrometry, they must be cut from the gel. Automate this process with the EXQuest™ spot cutter. Then import your cut list from the software and the instrument will do the rest.

     
    2-D Workflow

Applications and Technical Resources

The 2-D electrophoresis applications and technology page is a virtual encyclopedia of 2-D electrophoresis. You'll find extensive information, videos, and troubleshooting guides to ensure you get the best 2-D results possible.

On the 2-D Electrophoresis applications and technology page you can:

x

Account price failed to display. Please try reloading the page. Reload Page

If you are placing an order, you may proceed with your order; the account price will be applied if it is lower than the
list price. If the problem persists, please call customer service at   or send an
email to 

PROTEAN i12 IEF system
PROTEAN® i12™ IEF system

1646000
Isoelectric focusing system, includes basic unit, electrode assemblies, focusing and rehydration/equilibration trays, forceps, electrode wicks, mineral oil, cleaning accessories, IPG strips and sample/rehydration buffer, USB flash drives, styluses

List Price:
 
Inquire
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
ChemiDoc MP System
ChemiDoc™ MP System

1708280
Gel imaging system, includes darkroom, UV transilluminator, epi-white illumination, camera, power supply, cables, Image Lab™ software compatible with Windows or Mac OS

List Price:
 
Inquire
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
(Discontinued) Criterion™ Dodeca™ Cell and PowerPac™ Universal Power Supply

1654139
Vertical midi-format electrophoresis cell (#165-4130), 12 gel capacity, includes buffer tank with cooling coil, lid with power cables, universal power supply (#164-5070)

This product is no longer available. Please contact technical support for more information on replacement product.
EXQuest Spot Cutter

1657200
Gel excision instrument, includes enclosure, imaging system, fluidics system, robotics, sensors, cutting head, gel tray, microplate track, wash station

List Price:
 
Inquire
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
PDQuest Advanced 2-D Analysis Software

1709630
1-user license, 2-D analysis software, provides advanced functionality

List Price:
 
Inquire
Loading
Your Price:
 

Communication error

Reload Page

Your Price:   Log in
Quantity:   Add to cart
Add to Hot List   Add to Quote
Number Description Options
6478 2-D Electrophoresis Workflow Brochure, Rev B Click to download [ Add to Cart (Free) ]
6493 Bio-Rad’s Host Cell Protein (HCP) Workflow Quick Guide, Rev B Click to download [ Add to Cart (Free) ]
2651 2-D Electrophoresis Workflow How-To Guide, Rev F Click to download
5635 ProteoMiner Protein Enrichment Technology Bulletin, Rev B Click to download
10010636 Instruction Manual, ProteoMiner Protein Enrichment Kits, Rev D Click to download
5916 Enrichment of Medium- and Low-abundance Proteins in Sample Types Using ProteoMiner Technology, Rev A. Click to download
4110143 Instruction Manual, ReadyPrep 2-D Cleanup Kit, Rev A Click to download [ Add to Cart (Free) ]
4110009 Instruction Manual, ReadyPrep 2-D Starter Kit, Rev A Click to download
2642 ReadyPrep 2-D Starter Kit Product Information Sheet Click to download
6097 PROTEAN i12 IEF System Brochure, Rev A Click to download [ Add to Cart (Free) ]
6138 PROTEAN i12 IEF System: Independent Voltage and Current Control Enables Optimization of First-Dimension IEF Conditions, Rev A Click to download
4006166 Instruction Manual, ReadyStrip IPG Strip, Rev H Click to download
2442 ReadyStrip IPG Strip Product Information Sheet, Rev F Click to download
4006197 Instruction Manual, Criterion Dodeca Cell, Rev A Click to download
6040 Electrophoresis Guide, Interactive PDF, Rev B Click to download
6007 Criterion™ TGX™ Precast Gels Product Information Sheet, Rev B Click to download [ Add to Cart (Free) ]
4110001 Instruction Manual, Criterion™ Gel Application Guide, Rev F Click to download
5991 Oriole Fluorescent Gel Stain: A Novel Fluorescent Stain for a Simpler 2-D PAGE to MALDI Workflow, Rev A Click to download
10017295 Instruction Manual, Oriole Fluorescent Gel Stain, Rev B Click to download
5900 Oriole Fluorescent Gel Stain PIS, Rev A Click to download
6133 ChemiDoc™ MP Brochure, Rev C Click to download [ Add to Cart (Free) ]
3121 PDQuest 2-D Analysis Software Brochure, Rev C Click to download
3194 EXQuest Spot Cutter Brochure, Rev A Click to download [ Add to Cart (Free) ]
10001531 Instruction Manual, EXQuest™ Spot Cutter, Rev A Click to download
6371 Electrophoresis Power Supplies Brochure, Rev C Click to download [ Add to Cart (Free) ]
6393 Reliable, Streamlined 2-D Western Blot Workflow for Evaluation of Antibodies Developed for Detection of Host Cell Proteins, Rev A Click to download [ Add to Cart (Free) ]
6405 Effectiveness of 2-D Electrophoresis over 1-D Electrophoresis Followed by Western Blotting for Evaluating Strategies Used to Generate Host Cell Protein Antibodies, Rev A Click to download