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Chromatography Systems


Question:

Why are the peaks in the chromatograms broad, indistinct, or merged with other peaks?


Answer:

  • The gradient may need to be optimized in a bind and elute method.
  • There might be too much dead volume in the system resulting in a dilution effect. Try to minimize the volume of tubing and equipment between the sample inlet and the column.
  • The larger bead size typically used in low pressure chromatography media may not provide sufficient resolution. Smaller beads might be required for higher resolution.
  • If the separation technique is size exclusion, a higher pressure system may be required to maintain a steady flow rate.


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