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Transfer Systems | Membranes and Filter Paper |
Buffers and Reagents |
V3 Western Workflow™ |
PrecisionAb™ Antibodies |
Related Products | Resources |
FEATURED PRODUCTFluorescent Antibodies
- StarBright™ Blue 700 Antibodies for unmatched sensitivity and easy multiplexing
- hFAB™ Rhodamine Antibodies for one-step detection of housekeeping proteins (actin, tubulin, and GAPDH)
Western Blotting Transfer Systems
Trans-Blot® Turbo™ Transfer System
The Trans-Blot Turbo System enables fast, efficient, and reproducible transfer of proteins up to 400 kD. Preassembled Trans-Blot Turbo Transfer Packs are optimized for superior blot transfer.
- Fast transfer, in as little as 3 min
- Highest efficiency transfer
- High throughput — 4 mini gels or 2 midi gels per run
Semi-Dry and Rapid Blotting Systems
Wet/Tank Blotting Systems
- Mini Trans-Blot Cell —for 2 mini gels
- Criterion™ Blotter —for 2 midi or 4 mini gels
- Trans-Blot Cell —for 3 large, 6 midi, or 12 mini gels
- Trans-Blot Plus Cell —for 3 XL or 12 midi gels
Microfiltration and Screening Systems
Western Blotting Membranes and Filter Paper
Nitrocellulose Membranes
- Nitrocellulose Membrane 0.45 μm
- Nitrocellulose Membrane 0.2 μm
- Supported Nitrocellulose Membrane, 0.45 μm
- Supported Nitrocellulose Membrane, 0.2 μm
PVDF Membranes
Western Blotting Filter Paper
Immun-Blot PVDF Membrane
This hydrophobic PVDF membrane is ideal for chemiluminescent and colorimetric western blots. High mechanical strength facilitates reprobing.
- Retains target proteins very strongly
- Reduces nonspecific protein binding
- Ensures high signal with low background
Western Blotting Buffers and Reagents
Clarity™ and Clarity Max™ Western ECL Blotting Substrates
Bio-Rad’s Clarity family of Western ECL substrates are compatible with any HRP-conjugate secondary antibody and ideal for both digital and film-based imaging.
Clarity Substrate
- Excellent sensitivity with low background levels
- 24-hour signal duration
Clarity Max Substrate
- Highest level of sensitivity with low background levels
- Recommended for detection of low target amounts
Antibodies and Conjugates
- StarBright Blue 700 Fluorescent Antibodies
- hFAB Rhodamine Housekeeping Protein Fluorescent Antibodies
- PrecisionAb™ Validated Western Blotting Antibodies
- HRP and AP Conjugates
Detection Reagents
- Clarity and Clarity Max Western ECL Substrates
- Immun-Star™ AP Chemiluminescence Kits
- Immun-Blot® Opti-4CN™ Colorimetric Kits
- Immun-Blot AP Colorimetric Kits
Transfer Buffers
V3 Western Workflow™
The V3 Western Workflow streamlines the western blotting protocol, saving time and increasing accuracy and reliability by incorporating innovative tools throughout the process:
- Stain-free gel and blot imaging for total protein detection
- Rapid transfer with the Trans-Blot Turbo System
- High sensitivity and image resolution with the ChemiDoc™ MP and ChemiDoc Imaging Systems
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Learn more about the V3 Western Workflow
Related Products
Bio-Rad carries a full line of gel and blot imaging systems, including its proprietary stain-free imaging technology.
Gel and Blot Imaging Systems
ChemiDoc Imaging Systems
Gel Doc™ EZ and Gel Doc XR+
Systems
GS-900™ Calibrated ensitometer
(Note: This system is not enabled for stain-free detection)
Protein Electrophoresis Systems, Supplies, and Sample Preparation Kits
Western Blotting Resources
Guides
Western Blot Doctor™ Troubleshooting Guide
Our self-help troubleshooting guide covers solutions to many common and not-so-common western blotting issues and helps your blots look their best.
Protein Blotting Guide
(PDF 7.9 MB)
Details on blotting technology, available products, and methods, plus tips, techniques, and troubleshooting.
Electrophoresis Guide
(PDF 6.4 MB)
A guide to polyacrylamide gel electrophoresis and protein detection, including theory, product selection, protocols, and more.
Technical Resources
Western Blotting Techniques
Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
Protein Separation and Analysis
Bio-Rad's V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.
Stain-Free Western Blot Imaging
(VIDEO 11:41 mins)
See the fast, convenient, and transparent V3 Western Workflow process, how Image Lab Software performs data normalization using total protein loading controls, and how stain-free imaging yields reliable western blot results.
Single-Cell Western Blot and Stain-Free Total Protein Loading Control
(VIDEO 45:55 mins)
Demonstration of quantitative analysis of fiber type–specific protein expression patterns in single muscle cells with a unique western blotting protocol utilizing Bio-Rad's stain-free imaging technology.
Can We Trust Western Blots?
(VIDEO 45:41 mins)
In this presentation, Aldrin Gomes, Associate Professor of Neurobiology at UC Davis, talks about key success factors to instill trust in western blotting data.
Protein Electrophoresis
Find protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.
Imaging and Analysis
Find information on protein visualization and quantitation methods, gel and blot imaging instrumentation, and image analysis software.
Protocols
- Determining the Appropriate Sample Load for Western Blots (PDF 180 KB)
- Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow (PDF 182 KB)
- V3 Western Workflow (PDF 589 KB)
- Validating the Expression Consistency of a Housekeeping Protein (PDF 198 KB)
- General Protocol for Western Blotting (PDF 96 KB)
- General V3 Western Workflow Protocol (PDF 578 KB)
Publication Listings
- Total Protein Normalization Publication List (PDF 197 KB)